“…So, its analytical estimation/determination methods become more important for its regulation. High Performance Liquid Chromatography (HPLC), [8,9] Thin layer chromatography (TLC), [10] high-performance thinlayer chromatography (HPTLC), [11] reversed-phase thin layer chromatography (RPTLC), [12] isotachophoresis [13] and UV-spectrophotometric [14] methods for determination of caffeine either in bulk, dosage forms, herbal products, energy drinks or in biological fluids have been described previously. Herein, we have developed and validated the HPTLC -UV method, for the quick determination of caffeine from marketed granules of tea.…”