Determination of Caffeine in Saliva by High-Performance Liquid Chromatography

Suzuki, Yoshinari; Uematsu, Toshihiko; Mizuno, Atsuhiro; Fujii, Kiichiro; Nakashima, Mitsuyoshi

doi:10.1097/00007691-198901000-00018

Cited by 10 publications

(7 citation statements)

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“…For all collection methods, there was good correlation between salivary and plasma concentrations but the saliva sampling method using citric acid in the infant's cheek pouch before collection showed the strongest correlation with plasma caffeine concentrations . However, this technique carries the risk of dilution of the sample by citric acid solution, which may reduce repeatability . In the present study using a commercially available salivary collection system, adequate volumes of saliva were collected without the administration of citric acid.…”

Section: Discussionmentioning

confidence: 83%

Salivary caffeine concentrations are comparable to plasma concentrations in preterm infants receiving extended caffeine therapy

Dobson

Liu

Rhein

et al. 2016

Brit J Clinical Pharma

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Section: Discussionmentioning

confidence: 83%

Salivary caffeine concentrations are comparable to plasma concentrations in preterm infants receiving extended caffeine therapy

Dobson

Liu

Rhein

et al. 2016

Brit J Clinical Pharma

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“…The development, validation and application of this method demonstrate several key advantages of this technique: the method is easily introduced into most laboratories with an HPLC system since no specialized equipment is required (Suzuki et al. , 1989); only minor (volumetric) adjustments are needed when alternating between the method for plasma and saliva; only relatively small volumes of saliva (100 µL) and plasma (200 µL) are needed; a high degree of accuracy and precision are attained; and it is the only method available which validates (presenting data) not only caffeine but also its major metabolite of interest, paraxanthine, in both plasma and saliva.…”

Section: Discussionmentioning

confidence: 99%

Caffeine and paraxanthine HPLC assay for CYP1A2 phenotype assessment using saliva and plasma

Perera

Gross

McLachlan

2010

Biomedical Chromatography

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Caffeine has been extensively used as a probe to measure CYP1A2 activity in humans with caffeine clearance or the paraxanthine (major metabolite of caffeine) to caffeine concentration ratio being regarded as the preferred metric. A simple reverse-phased C(18) HPLC assay using ethyl acetate liquid-liquid extraction was developed to quantitate caffeine and paraxanthine concentrations in saliva and plasma. The mobile phase consisted of acetonitrile-acetic acid-H(2)O (100:1:899) and analytes were quantitated with UV detection at 280 nm. The extraction recovery for paraxanthine and caffeine was approximately 70% in both saliva and plasma. The assay was linear over the concentration ranges 0.05-2.50 and 0.05-5.00 µg/mL, for paraxanthine and caffeine, respectively, in saliva. In plasma the assay was linear over the ranges 0.025-2.50 and 0.025-5.00 µg/mL for paraxanthine and caffeine, respectively. Intra- and inter-assay precision and accuracy were less than 15%. Detection limits were 0.015 µg/mL for paraxanthine and caffeine in saliva, while it was 0.005 µg/mL for paraxanthine and caffeine in plasma. Utility was established in samples collected from two healthy volunteers who abstained from caffeine for 24 h and received a single 100 mg oral dose of caffeine. The assay developed is a robust, simple and precise technique to measure caffeine and paraxanthine in saliva and plasma of healthy volunteers after a single oral dose of caffeine.

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“…Saliva sampling is considered a good method for frequent measurement of caffeine pharmacokinetics (Newton et al, 1981;Suzuki et al, 1989), with a linear relationship reported between caffeine concentrations in saliva and plasma, and salivary caffeine levels reported to be around 80% of plasma levels (Perera et al, 2011;Zylber--Katz et al, 1984). Collection, storage and analysis procedures are supplied as Supplementary Material.…”

Section: Salivary Caffeine Concentrationsmentioning

confidence: 99%

The effect of caffeine on working memory load-related brain activation in middle-aged males

et al. 2013

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Determination of Caffeine in Saliva by High-Performance Liquid Chromatography

Cited by 10 publications

References 0 publications

Salivary caffeine concentrations are comparable to plasma concentrations in preterm infants receiving extended caffeine therapy

Salivary caffeine concentrations are comparable to plasma concentrations in preterm infants receiving extended caffeine therapy

Caffeine and paraxanthine HPLC assay for CYP1A2 phenotype assessment using saliva and plasma

The effect of caffeine on working memory load-related brain activation in middle-aged males

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