Determination of calibration parameters of cantilevers of arbitrary shape by finite element analysis

Rodriguez-Ramos, Jorge; Rico, Félix

doi:10.1063/5.0036263

Cited by 9 publications

(5 citation statements)

References 34 publications

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“…All experiments were performed in HEPES-buffered solution, pH 7.4. Measurements were obtained on a Bruker MultiMode 8 AFM in contact mode using a cantilever (PFQNM-LCA-CAL, Bruker) ( 57, 58 ) with a tip radius of 70 nm and a nominal spring constant of 0.1 N/m. Force-deflection curves were obtained using Nanoscope III analysis Software for each aorta at 2 different areas per aorta.…”

Section: Discussionmentioning

confidence: 99%

Mechanosensing through talin 1 contributes to tissue mechanical homeostasis

Chanduri,

Kumar,

Weiss

et al. 2023

Preprint

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It is widely believed that tissue mechanical properties, determined mainly by the extracellular matrix (ECM), are actively maintained. However, despite its broad importance to biology and medicine, tissue mechanical homeostasis is poorly understood. To explore this hypothesis, we developed mutations in the mechanosensitive protein talin1 that alter cellular sensing of ECM stiffness. Mutation of a novel mechanosensitive site between talin1 rod domain helix bundles 1 and 2 (R1 and R2) shifted cellular stiffness sensing curves, enabling cells to spread and exert tension on compliant substrates. Opening of the R1-R2 interface promotes binding of the ARP2/3 complex subunit ARPC5L, which mediates the altered stiffness sensing. Ascending aortas from mice bearing these mutations show increased compliance, less fibrillar collagen, and rupture at lower pressure. Together, these results demonstrate that cellular stiffness sensing regulates ECM mechanical properties. These data thus directly support the mechanical homeostasis hypothesis and identify a novel mechanosensitive interaction within talin that contributes to this mechanism.

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Section: Discussionmentioning

confidence: 99%

Mechanosensing through talin 1 contributes to tissue mechanical homeostasis

Chanduri,

Kumar,

Weiss

et al. 2023

Preprint

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“…The thermal noise method was used, 59 applying a correction factor of 0.817 for V-shaped cantilevers. 60 The spring constants determined varied between 0.01 and 0.014 N m −1 and the sensitivities ranged from 43.71 to 54.96 nm V −1 . Several independent experiments were performed using independently functionalized cantilevers and coverslips.…”

Section: ■ Materials and Methodsmentioning

confidence: 97%

“…Cantilevers were calibrated for each retract speed as the cantilever properties changed in the NiCl 2 solution. The thermal noise method was used, applying a correction factor of 0.817 for V-shaped cantilevers . The spring constants determined varied between 0.01 and 0.014 N m –1 and the sensitivities ranged from 43.71 to 54.96 nm V –1 .…”

Section: Methodsmentioning

confidence: 99%

Heterogeneous and Cooperative Rupture of Histidine–Ni²⁺ Metal-Coordination Bonds on Rationally Designed Protein Templates

Khare,

Gonzalez Obeso,

Martín-Moldes

et al. 2024

ACS Biomater. Sci. Eng.

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Metal-coordination bonds, a highly tunable class of dynamic noncovalent interactions, are pivotal to the function of a variety of protein-based natural materials and have emerged as binding motifs to produce strong, tough, and self-healing bioinspired materials. While natural proteins use clusters of metal-coordination bonds, synthetic materials frequently employ individual bonds, resulting in mechanically weak materials. To overcome this current limitation, we rationally designed a series of elastin-like polypeptide templates with the capability of forming an increasing number of intermolecular histidine−Ni 2+ metal-coordination bonds. Using single-molecule force spectroscopy and steered molecular dynamics simulations, we show that templates with three histidine residues exhibit heterogeneous rupture pathways, including the simultaneous rupture of at least two bonds with more-thanadditive rupture forces. The methodology and insights developed improve our understanding of the molecular interactions that stabilize metal-coordinated proteins and provide a general route for the design of new strong, metal-coordinated materials with a broad spectrum of dissipative time scales.

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“…117 For that, we used the pre-calibrated spring constant of the cantilevers and the correction factors described in Rodriguez-Ramos. 118…”

Section: Methodsmentioning

confidence: 99%

Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocyte differentiation into macrophages

et al. 2023

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Determination of calibration parameters of cantilevers of arbitrary shape by finite element analysis

Cited by 9 publications

References 34 publications

Mechanosensing through talin 1 contributes to tissue mechanical homeostasis

Mechanosensing through talin 1 contributes to tissue mechanical homeostasis

Heterogeneous and Cooperative Rupture of Histidine–Ni²⁺ Metal-Coordination Bonds on Rationally Designed Protein Templates

Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocyte differentiation into macrophages

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Determination of calibration parameters of cantilevers of arbitrary shape by finite element analysis

Cited by 9 publications

References 34 publications

Mechanosensing through talin 1 contributes to tissue mechanical homeostasis

Mechanosensing through talin 1 contributes to tissue mechanical homeostasis

Heterogeneous and Cooperative Rupture of Histidine–Ni2+ Metal-Coordination Bonds on Rationally Designed Protein Templates

Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocyte differentiation into macrophages

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Product

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Heterogeneous and Cooperative Rupture of Histidine–Ni²⁺ Metal-Coordination Bonds on Rationally Designed Protein Templates