Determination of Carbohydrates Content in Gentiana Cruciata L. By Gc/MS Method

Budniak, Liliia; Slobodianiuk, Liudmyla; Marchyshyn, S. М.; Klepach, Pavlina; Honcharuk, Yana

doi:10.22159/ijap.2021v13i1.39820

Cited by 10 publications

(10 citation statements)

References 39 publications

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“…The raw material was authenticated by prof. Svitlana Marchyshyn (TNMU, Ternopil, Ukraine). The study plant material was dried using conventional method and stored in paper bags in a dry place [14].…”

Section: Materials and Methods 1 Plant Materialsmentioning

confidence: 99%

Determination of composition of fatty acids in Saponaria officinalis L.

Budniak¹,

Slobodianiuk²,

Marchyshyn³

et al. 2021

SR: PS

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Treatment using medicinal plants with a long history of use is of interest to our society. These plants include Saponaria ofﬁcinalis L., as well commonly known as common soapwort belongs to the family Caryophyllaceae. The herb and roots of this plant used as a blood purifier, an expectorant in bronchitis, diaphoretic and diuretic, for skin diseases, to increase bile flow. The plant contains various secondary metabolites, but there is no information on the fatty acids composition of Saponaria officinalis L. herb and roots. The aim. The aim of the present study was to determine the qualitative composition and quantitative content of fatty acids by gas chromatography/mass spectrometry method (GC/MS) in Saponaria officinalis L. herb and roots. Materials and methods. The determination of fatty acids composition of Saponaria officinalis L. herb and roots were carried out by gas chromatograph Agilent 6890N (Agilent Technologies, USA). Results. The research of Saponaria ofﬁcinalis L. herb showed a mixture of unsaturated (1.9 mg/g) and saturated (1.27 mg/g) fatty acids. The main components of this raw material were linolenic (1.15 mg/g), linoleic (0.75 mg/g) and heneicosylic (0.38 mg/g) acids. The main components of this raw material were palmitic (0.38 mg/g), linoleic (0.16 mg/g) and linolenic (0.09 mg/g) acids. Conclusions. As a result of Saponaria officinalis L. study, the presence of fatty acids is established in herb and roots. Using the GC/MS method determined the qualitative composition and quantitative content of fatty acids in study raw material. Twelve fatty acids were determined in the herb of Saponaria officinalis L. The dominant fatty acids in the studied raw material were linolenic and linoleic acids, their content was 1.15 mg/g and 0.75 mg/g, respectively. Nine fatty acids were determined in the Saponaria officinalis L. roots. The palmitic acid prevailed among fatty acids, it is content was 0.38 mg/g. Our findings suggest that Saponaria officinalis L. is a promising plant because of the important role of fatty acids in different biological processes

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Section: Materials and Methods 1 Plant Materialsmentioning

confidence: 99%

Determination of composition of fatty acids in Saponaria officinalis L.

Budniak¹,

Slobodianiuk²,

Marchyshyn³

et al. 2021

SR: PS

Self Cite

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“…Injections of 1 μL were made in the split mode 1:50. The detection was held in the SCAN mode in the range of 38-400 m/z (Budniak et al 2021;Savych et al 2021f).…”

Section: Chromatographic Conditionmentioning

confidence: 99%

Analysis of carbohydrates content in the plant components of antidiabetic herbal mixture by GC-MS

Savych¹,

Дученко²,

Shepeta³

et al. 2021

PHAR

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Medicinal plants and their combinations due to the wide range of biologically active substances can influence on various links of the pathogenetic mechanism of development of diabetes mellitus and its complications. One of such combinations is an antidiabetic herbal mixture (Urticae folia, Rosae frucrus, Myrtilli folia, Menthae folia and Taraxaci radices) with established hypoglycemic, hypolipidemic, antioxidant, hepatoprotective, pancreatoprotective activity in previous pharmacological study in vivo. Thus, the aim of this study was to identify and establish the content of carbohydrates in free and bound form in the plant components of antidiabetic herbal mixture. The carbohydrates were separated by gas chromatography-mass spectrometry after conversion into volatile derivatives as aldononitrile acetate. The monomeric composition of polysaccharides was studied after their hydrolysis to form monosaccharides and polyalcohols. The results of the quantitative study showed that the predominant carbohydrate in free form was saccharose in Urticae folia, L-fructose in Myrtilli folia, Rosae frucrus, Taraxaci radices and Menthae folia, L-glucose in Rosae frucrus. Concerning the determination of monomers of polysaccharides after hydrolysis, L-glucose prevailed in all plant components of antidiabetic herbal mixture. The chromatographic study revealed a number of polyalcohols that are important for the treatment and prevention of progression of diabetes mellitus and its complications, namely, mannitol and myo-inositol.

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“…Identification of monosaccharides was based on their retention times compared to standards and mass library NIST 02. Quantification was done by using internal standard of sorbitol added to the sample (Slobodianiuk et al 2019;Huzio et al 2020;Budniak et al 2021a).…”

Section: Sample Preparationmentioning

confidence: 99%

Analysis of carbohydrates in Saponaria officinalis L. using GC/MS method

Slobodianiuk¹,

Budniak²,

Marchyshyn³

et al. 2021

PHAR

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Saponaria officinalis L. (common soapwort), usually named fuller’s herb, is encountered in most of Europe, in Spain, France, Italy, for example, and also in Syria and North Africa. Saponaria officinalis L. is known in most of the world as an introduced species, often a weed, and sometimes as cultivated decorative plant. Saponaria officinalis contains a large amount of saponins, which foam during extraction with water. In addition to saponins, common soapwort also contains flavonoids, quillaic acid, fatty acids and different phenolic compounds. There is a lack of information about carbohydrates content of common soapwort. Thus the aim of this study was to determine the content of carbohydrates Saponaria officinalis L. herb and roots. The qualitative composition and quantitative content of carbohydrates in herb and roots of Saponaria officinalis L. were determined by using GC/MS method. The studies have shown that Saponaria officinalis L. herb is mainly composed of free carbohydrates such as D-glucose (3.65 mg/g), D-galactose (0.29 mg/g), D-fructose (0.20 mg/g) and D-saccharose (3.72 mg/g). In common soapwort herb, after acidic hydrolysis and derivatization with acetylated aldononitriles, D-arabinose, D-fucose, D-mannose, D-glucose, D-galactose, D-fructose and Myo-inositol were identified. Free carbohydrates in the roots of Saponaria officinalis L., including D-glucose, D-galactose and D-saccharose, were determined with GC/MS method too. D-saccharose was common among free carbohydrates of Saponaria officinalis L. in the largest amounts. Its content in herb and roots of the common soapwort was 3.72 mg/g and 25.39 mg/g respectively.

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Determination of Carbohydrates Content in Gentiana Cruciata L. By Gc/MS Method

Cited by 10 publications

References 39 publications

Determination of composition of fatty acids in Saponaria officinalis L.

Determination of composition of fatty acids in Saponaria officinalis L.

Analysis of carbohydrates content in the plant components of antidiabetic herbal mixture by GC-MS

Analysis of carbohydrates in Saponaria officinalis L. using GC/MS method

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