Determination of carbohydrates in medicinal plants‐comparison between TLC, mf‐MELDI‐MS and GC‐MS

Abstract

Evaluation of all three techniques employed, clearly proved the heightened performance of mf-MELDI-MS for the qualitative analysis of complex mixtures, as targets do not need modification and analysis requires only a few minutes. In addition, GC-MS is suitable for quantitative analysis.

Metabolomics in Herbal Medicine Research

“…TOF instruments are the most commonly used mass analyzers and have the fastest scan rate, and are able to acquire a significant number of spectra across each peak, thus leading to higher sensitivity and better spectral quality [46]. GC-MS has been used as an efficient method for separating and identifying volatile oils, natural products such as mono-and sesquiterpenes, and oligosaccharides in several Chinese medicinal herbs [47,48]. Plant metabolites cover a huge range of polarity; therefore, to cover as many metabolites as possible in a single GC-MS run, in many cases a derivatization step is needed to obtain maximum compound coverage.…”

Quality control of sweet medicines based on gas chromatography-mass spectrometry

“…Compared with free carbohydrates, polymeric c a r b o h y d r a t e s s u c h a s o l i g o s a c c h a r i d e s a n d polysaccharides are more difficult to quantify due to their large molecular weights, complex structures and rare of chemical standards (4). However, separation and quantification are possible for free carbohydrates (including mono-, di-and trisaccharides) by GC-MS (24,112). Therefore, suitable hydrolysis of polymeric carbohydrates (discussed in Section 2.2) combined with efficient derivatization methods (discussed in Section 2.1) is an alternative method to quantify the carbohydrates in sweet medicines for QC.…”

“…Taking the advantage of high efficiency of microwave, the derivatization procedure could be significantly shortened. Silylation was finished within 4 min with HMDS, BSTFA or MSTFA as derivative reagents (24). Microwave-assisted derivatization combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (2D GC-TOF-MS) has been successfully applied for carbohydrate analysis in complex extracts (57).…”

Quantification of methyl esters of fatty acids in the oil of Physalis minima by GC-MS

“…This makes a base for the investigation of metabolites which have various biological and pharmacological importance [1,4,7,8]. Fatty acids, the basic structural units of lipid, are among the important quality markers which have biological and pharmacological importance, such as, for the evaluation of nutritional value of food, enhancing immunity and for lowering the risks of heart related diseases [7][8][9][10][11][12][13][14][15][16][17][18].…”

“…Qualitative and quantitative evaluation of the chemical constituents is vital for establishing the quality control profile and standardization of herbal extracts [1][2][3][4][5][6]. This makes a base for the investigation of metabolites which have various biological and pharmacological importance [1,4,7,8].…”