Determination of carnosic acid and rosmarinic acid in sage by capillary electrophoresis

Başkan, Selda; Öztekin, Nevin; Erim, F. Bedia

doi:10.1016/j.foodchem.2006.01.033

Cited by 86 publications

(57 citation statements)

References 12 publications

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“…officinalis L. (family Lamiaceae), commonly called rosemary, is a culinary as well as medicinal herb with biological activities. 23 It is native to the Mediterranean region and is now widely distributed in European countries. 15 It has an ancient reputation for improving memory, and has been used as a symbol for remembrance in Europe.…”

Section: Discussionmentioning

confidence: 99%

“…CA is a phenolic diterpene compound found in sage (2-5 mg CA/g sage) and rosemary (12-15 mg CA/g rosemary). 22,23 The major active compounds of rosemary for control of cell migration can be considered as the phenolic diterpenes CA and CAR.…”

Section: Discussionmentioning

confidence: 99%

“…22,23 As shown in Table 1, the CA contents in RM and RH were higher compared with CAR. The content of CA in RH (18.4%, 184.00 lg/mg dry wt.)…”

Section: Contents Of Ca and Carmentioning

confidence: 94%

“…21 CA and CAR have a typical O-diphenol structure. 22,23 CA and CAR also exhibit anti-inflammatory, anticancer, and scavenging activities. 24 The antiatherosclerotic mechanism(s) of rosemary are illunderstood.…”

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confidence: 99%

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Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Chae

et al. 2012

Journal of Medicinal Food

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Atherosclerosis is a chronic and progressive inflammatory disease. Novel anti-inflammatory therapies may have promise as treatment strategies for cardiovascular risk reduction. Rosemary (Rosemarinus officinalis L.) has been used in folk medicine to treat headaches, epilepsy, poor circulation, and many other ailments. It was found that rosemary could act as a stimulant and mild analgesic and could reduce inflammation. However, the mechanisms underlying the anti-inflammatory and antiatherosclerotic effects of rosemary need more study. This study investigated effects of the rosemary components, carnosic acid (CA), and carnosol (CAR), on cell migration. Monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) were determined by Western blot and gelatin zymography, respectively, in RAW 264.7 macrophages and vascular smooth muscle cells (VSMCs). VSMC migration was assessed by a Matrigel migration assay. Active compounds of rosemary extracts were also analyzed using a reversed-phase high-performance liquid chromatography. MMP-9 and MCP-1 activities were markedly diminished with methanol extract (RM), n-hexane fraction (RH), and CA in RAW 264.7 cells. RM, RH, CA, and CAR suppressed tumor necrosis factor-alpha-induced VSMC migration by inhibiting MMP-9 expression. Chromatograms of RM-and RH-containing CA and CAR revealed higher CA contents of RM (9.4%, 93.85 lg/mg dry wt.) and, especially, RH (18.4%, 184.00 lg/mg dry wt.), which were appreciably elevated compared with the similar CAR content in RM and RH (3.7%, 37.30 lg/mg dry wt.; and 2.5%, 25.05 lg/mg dry wt., respectively). Rosemary, especially its CA component, has potential antiatherosclerosis effects related to cell migration.KEY WORDS: Rosemarinus officinalis L. rosemary matrix metalloproteinase-9 (MMP-9) monocyte chemoattractant protein-1 (MCP-1) migration RAW 264.7 macrophages vascular smooth muscle cell

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…22,23 As shown in Table 1, the CA contents in RM and RH were higher compared with CAR. The content of CA in RH (18.4%, 184.00 lg/mg dry wt.)…”

Section: Contents Of Ca and Carmentioning

confidence: 94%

mentioning

confidence: 99%

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Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Chae

et al. 2012

Journal of Medicinal Food

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“…The common methods used for the determination of RA are high performance liquid chromatography (HPLC) (Hong and Kim 2010) and spectrophotometry (Öztürk et al 2010). Other methods described in the literature are based on Fourier transform infrared (FT-IR) spectroscopy (Saltas et al 2013), capillary electrophoresis (Başkan et al 2007) or gas chromatography-mass • Voltammetric investigation of rosmarinic acid on a cheap, disposable pencil graphite electrode (PGE) • Rapid, simple and sensitive voltammetric method on PGE for rosmarinic acid quantification • Cheap tool for the rapid assay of the total polyphenolic content of teas (mg RAE/g tea) • Precision of the developed voltammetric method comparable to that of Folin-Ciocalteu method * Mihaela Buleandră mihaelabuleandra@yahoo.com spectrometry (GC-MS) (Razboršek 2011). Many laboratories determine the total polyphenolic content (TPC) in different matrices using the Folin-Ciocalteu method (Shanker and Debnath 2015) which is based on their chemical reducing capacity relative to gallic acid as standard, in most cases (Singleton et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Voltammetric determination of polyphenolic content as rosmarinic acid equivalent in tea samples using pencil graphite electrodes

et al. 2016

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The quasi-reversible, diffusion controlled behavior of rosmarinic acid (RA) on a disposable pencil graphite electrode (PGE) was established by cyclic voltammetry. Using the anodic oxidation peak presented by RA on the PGE a differential pulse voltammetric (DPV) method was developed for the quantitative determination of RA. The linear range was 10 −8 -10 −5 M RA and the detection and quantification limits were 7.93 × 10 −9 M and 2.64 × 10 −8 M RA, respectively. The applicability of the developed method was tested by recovery studies and by the assessment of the total polyphenolic contents (TPC DPV ) of green, white and black Turkish teas, which were found to be 40.74, 30.04 and 23.97 mg rosmarinic acid equivalent/g dry tea, respectively. These results were in good agreement with those obtained by the Folin-Ciocalteu method. The developed method is a sensitive and cheap tool for the rapid and precise evaluation of TPC DPV of tea samples.

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High‐performance liquid chromatography method for determination of carnosic acid in rat plasma and its application to pharmacokinetic study

Yan

Wang

et al. 2009

Biomedical Chromatography

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A sensitive and reproducible high-performance liquid chromatography method with ultraviolet detection (UV) was developed for the determination of carnosic acid (CA) in rat plasma. After simple acidification and liquid-liquid extraction of plasma samples using gemfibrozil as an internal standard, the supernatant was evaporated to dryness under a gentle stream of nitrogen. The residue was reconstituted in 200 microL before being injected into the chromatographic system. The analysis was performed on a C(18) column protected by an ODS guard column using acetonitrile-0.1% phosphoric acid (55:45, v/v) as mobile phase, and the wavelength of the UV detector was set at 210 nm. The calibration curve was linear over the range of 0.265-265.0 microg/mL with a correlation coefficient of 0.9997. The recovery for plasma samples of 0.530, 13.25, 132.5 and 265.0 microg/mL was 72.2, 87.9, 90.4 and 94.7%, respectively. The intra-day and inter-day relative standard deviations for the measurements of quality control samples were less than 3.1%. The stability of the plasma samples was also validated. This method was successfully used to study the pharmacokinetics and bioavailability of CA in rats.

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Determination of carnosic acid and rosmarinic acid in sage by capillary electrophoresis

Cited by 86 publications

References 12 publications

Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Voltammetric determination of polyphenolic content as rosmarinic acid equivalent in tea samples using pencil graphite electrodes

High‐performance liquid chromatography method for determination of carnosic acid in rat plasma and its application to pharmacokinetic study

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