Determination of Catechol by a Novel Laccase Biosensor Based on Zinc-Oxide Sol-Gel

Qu, Jianying; Lou, Tongfang; Wang, Yong; Dong, Ying; Xing, Huanhuan

doi:10.1080/00032719.2014.1003427

Cited by 16 publications

(4 citation statements)

References 38 publications

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“…In order to evaluate the novelty and superiority of the biosensor, we made the comparison table for analytical performance of the as-prepared biosensor with previously reported laccase biosensors for CC and the comparative results are shown in Table 1. The comparative results clearly show that the as-prepared laccase biosensor exhibited a lower LOD (85 nM) towards CC than previously reported CC biosensors based on laccase immobilized on reduced graphene oxide supported palladium–copper alloyed nanocages42, nitrogen-doped ordered mesoporous/PVA matrix43, copper-containing ordered mesoporous carbon/chitosan matrix44, electrospun copper/carbon composite nanofibers45, 1-aminopyrene functionalized reduced graphene oxide46, carbon nanotubes–chitosan composite47, polyaniline48, multi-walled carbon nanotubes49 and ZnO sol-gel/chitosan modified electrodes50. However, the LOD of the as-fabricated CC biosensor is higher than the LOD (76 nM) of previously reported CC biosensor based on laccase immobilized reduced graphene oxide–glycol chitosan nanohybrid modified electrode, yet the sensitivity and linear response range of our biosensor is more comparable for the determination of CC.…”

Section: Resultsmentioning

confidence: 77%

A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol

Palanisamy

Ramaraj²,

Yang

et al. 2017

Sci Rep

118

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In the present work, we demonstrate the fabrication of laccase biosensor to detect the catechol (CC) using laccase immobilized on graphene-cellulose microfibers (GR-CMF) composite modified screen printed carbon electrode (SPCE). The direct electrochemical behavior of laccase was investigated using laccase immobilized different modified SPCEs, such as GR/SPCE, CMF/SPCE and GR-CMF/SPCE. Compared with laccase immobilized GR and CMF modified SPCEs, a well-defined redox couple of CuI/CuII for laccase was observed at laccase immobilized GR-CMF composite modified SPCE. Cyclic voltammetry results show that the as-prepared biosensor has 7 folds higher catalytic activity with lower oxidation potential towards CC than SPCE modified with GR-CMF composite. Under optimized conditions, amperometric i-t method was used for the quantification of CC, and the amperometric response of the biosensor was linear over the concertation of CC ranging from 0.2 to 209.7 μM. The sensitivity, response time and the detection limit of the biosensor for CC is 0.932 μMμA−1 cm−2, 2 s and 0.085 μM, respectively. The biosensor has high selectivity towards CC in the presence of potentially active biomolecules and phenolic compounds. The biosensor also accessed for the detection of CC in different water samples and shows good practicality with an appropriate repea.

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Section: Resultsmentioning

confidence: 77%

A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol

Palanisamy

Ramaraj²,

Yang

et al. 2017

Sci Rep

118

View full text Add to dashboard Cite

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“…The peaks of current signals are increased with enhancing ν (figure 6a) and the square root of ν (figure 6b). The correlation coefficient for the plot of currents vs. ν 1/2 is higher than that of it for currents vs. ν, demonstrating that a diffusion-controlled process overcomes the surface adsorption-controlled process [40,41]. ν influenced the cathodic and anodic peak potentials of the system, and as can be seen, the redox potentials were increased by increasing the logarithm of ν (log ν) (figure 7a).…”

Section: Voltammetric Responsementioning

confidence: 92%

Laccase immobilized onto graphene oxide nanosheets and electrodeposited gold–cetyltrimethylammonium bromide complex to fabricate a novel catechol biosensor

et al. 2019

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In this study, a new biosensor is developed with reliable and easy-to-use biodevice properties for catechol determination in real samples. A method is proposed for the fabrication of biosensors to sense catechol based on the adsorption method of laccase immobilization. Hence, a glassy carbon electrode was modified via graphene oxide nanosheets and then it was modified with a gold-cetyltrimethylammonium bromide nanocomposite to adsorb and immobilize laccase on the electrode surface. The results showed laccase immobilization onto the reformed glassy carbon electrode, and a direct electron transfer reaction between laccase and the electrode. The mechanism of electron transferring was EC. Also, k s and α were calculated as 0.41 s −1 and 0.33, respectively. For this biosensor two linear ranges, 0.1 × 10 −6 to 5 × 10 −6 M and 16.7 × 10 −6 to 166 × 10 −6 M, and a detection limit of 1.5 × 10 −6 M were obtained.

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“…The US Environmental Protection Agency (EPA) and the European Union (EU) consider it as an environmental pollutant due to its high toxicity to organisms . Thus, catechol determination is very important in medicine, physiology, and also in environmental protection fields . The common analytical techniques available to measure the concentration of phenol derivatives include spectrophotometry , capillary electrophoresis , liquid chromatography , and gas chromatography .…”

Section: Introductionmentioning

confidence: 99%

A novel sensitive laccase biosensor using gold nanoparticles and poly L‐arginine to detect catechol in natural water

Maleki

Kashanian

Nazari

et al. 2019

Biotech and App Biochem

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In this study, the simple, green, and fast layer-by-layer modification of the glassy carbon electrode was mainly performed by electrodeposition of gold nanoparticles and then, poly-L-arginine, and finally, laccase was covalently bonded to poly-L-arginine using glutaraldehyde. This type of fabrication is used for the first time for catechol detection, which provides a bioelectrocatalytic cycle for electron transport in the presence of laccase that results in sensitive and fast detection of catechol. The scanning electron microscopy, Fourier-transform infrared spectroscopy, and electrochemical studies were performed to confirm successful immobilization of the enzyme. The biosensor response was linear in a wide range of catechol trace concentrations, 24.90-274.00 nM, with the detection limit of 18.00 nM. Values of K m , α, n, and K s for the immobilized enzyme were calculated to be 1.25 × 10 −2 µM, 0.56, 3.19, and 0.28 Sec −1 , respectively. It was examined in real sample successfully confirming it is capable of measuring catechol in natural water. C 2019

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Determination of Catechol by a Novel Laccase Biosensor Based on Zinc-Oxide Sol-Gel

Cited by 16 publications

References 38 publications

A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol

A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol

Laccase immobilized onto graphene oxide nanosheets and electrodeposited gold–cetyltrimethylammonium bromide complex to fabricate a novel catechol biosensor

A novel sensitive laccase biosensor using gold nanoparticles and poly L‐arginine to detect catechol in natural water

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