Determination of CD4+ and CD8+ T cells in the peripheral blood of dogs with demodicosis

Abstract

The aim of this study was to evaluate the CD4+/CD8+ ratio in peripheral blood of dogs with localized and generalized demodicosis. Sixteen dogs were examined, 8 with localized and 8 with generalized demodicosis, while 8 healthy dogs were used as controls. Peripheral blood was obtained and CD4+ and CD8+ T cells were determined by flow cytometry. Significantly higher numbers of CD8+ T cells and lower numbers of CD4+ T cells were found in dogs with generalized demodicosis compared to dogs with localized demodicosis and healthy controls. Significantly higher numbers of CD8+ T cells and lower numbers of CD4+ T cells were also found in dogs with localized demodicosis compared to healthy controls. The CD4+/CD8+ ratio was also found to be significantly lower in dogs with generalized demodicosis in comparison with dogs with localized demodicosis and healthy controls. It is concluded that significant alteration in the CD4+/CD8+ ratio may be implicated in the pathogenesis of generalized canine demodicosis.

Demodex canis targets TLRs to evade host immunity and induce canine demodicosis

“…TGF‐β mRNA expression in peripheral blood mononuclear cells was higher in dogs with LD than healthy ones, and the key role of TGF‐β in the differentiation of LD and GD was suggested . It has also been demonstrated that dogs with GD have a lower CD4+ /CD8+ T cell ratio than dogs having LD . Therefore, increased TGF‐β1 concentration in dogs with GD may be associated with an increase in CD8+ T cell population in dogs with GD .…”

Prebiotic potential of Jerusalem artichoke (Helianthus tuberosus L.) in Wistar rats: effects of levels of supplementation on hindgut fermentation, intestinal morphology, blood metabolites and immune response

“…Immunophenotyping of peripheral blood lymphocyte T‐cell subsets (CD3, general T‐cell marker; CD4+, helper T‐cells; CD8+, cytotoxic/suppressor T‐cells) was performed with a dual‐laser benchtop FACScan flow cytometer (FACSCalibur, Becton Dickinson, San Diego, CA, USA) as described previously . Analysis was performed with CellQuest Pro‐software (BD Biosciences, San Jose, CA, USA).…”

Treatment of canine generalized demodicosis associated with hyperadrenocorticism with spot-on moxidectin and imidacloprid

“…Adult-onset canine generalized demodicosis is a multifactorial disease with an etiology including genetic factors and suppressed T-cell immunity [2]. Its development is also associated with immunosuppressive treatments, neoplasia and medical conditions characterized by metabolic changes, such as hyperadrenocorticism, hypothyroidism and diabetes mellitus [3].…”