Determination of cefadroxil in rat plasma and urine using LC–MS/MS and its application to pharmacokinetic and urinary excretion studies

Determination of isoorientin levels in rat plasma after oral administration of Vaccinum bracteatum Thunb. methanol extract by high‐performance liquid chromatography‐tandem mass spectrometry

“…We examined the feasibility of the developed method by performing a pharmacokinetic study on VBME and isoorientin in rats. All animal experiments were performed in accordance with the Guidelines for Animal Care and Use issued by Gachon University, as described previously (Jin, Kim, Kim, & Maeng, ; Jin et al, ). To cannulate femoral arteries with a polyethylene tube (PE‐50; Clay Adams, Parsippany, NJ, USA) filled with heparinized saline (20 IU/mL), rats ( n = 4) were anesthetized with ketamine (45 mg/kg, i.m.)…”

“…Peak concentration ( C max ) and time to reach C max ( T max ) were read directly from individual isoorientin plasma concentration–time profiles. Other pharmacokinetic parameters were calculated by noncompartmental analysis using Winnonlin 5.0.1 (Pharsight, Cary, NC, USA), as described previously (Jin et al, , ; Ahn & Maeng, ). Finally, the obtained pharmacokinetic parameters of isoorientin were compared for VBME and isoorientin treatments.…”

Determination of KD025 (SLx-2119), a Selective ROCK2 Inhibitor, in Rat Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Pharmacokinetic Application

“…The LOD was determined using the equation LOD = 3.3σ/m, where σ is the standard deviation of the intercept of the regression line, and m is the slope of the calibration curve [40,41]. It was also experimentally determined using the criterion of a signal-to-noise ratio of 3:1.…”

“…Sprague-Dawley rats (8-9 weeks, 250-300 g, Nara Biotech, South Korea) were provided free access to food and water and maintained under 12 h light/dark cycles to adjust to the laboratory environment for a week before commencing the study. Rats were anesthetized by intramuscular injections (20 mg/kg) of Zoletil®(Vibrac, TX, USA) and cannulated by surgery to the femoral artery with polyethylene tubing (Clay Adams, NJ, USA) filled with heparinized saline (20 IU/mL) for blood sampling [37,38,40]. After the rats had recovered from surgery, KD025 in a vehicle mixture (DMSO:Cremophor EL:PEG400:DDW = 5:10:40:50) was administered to rats at a dose of 5 mg/kg and 2 mg/kg for oral and IV administration, respectively.…”

New valid spectrofluorimetric method for determination of selected cephalosporins in different pharmaceutical formulations using safranin as fluorophore

“…The wide use of these antibiotics in modern antimicrobial therapy is due to their enhanced intrinsic microbiological activities and favorable safety profile. Chemically cephalosporin antibiotics are derived from the 7-aminocephalosporanic acid (7-ACA) which composed of a β-lactam ring fused with a dihydrothaizine ring (Table 1) Several methods have been reported for the quantitative determination of the cited cephalosporins including spectrophotometry [2][3][4][5][6][7][8], spectrofluorimetry [8][9][10][11][12][13][14], chemiluminescence [15], High performance liquid chromatography (HPLC) [16][17][18], Thin layer chromatography [19], capillary electrophoresis (CE) [20,21] and voltammetry [22][23][24][25][26].…”