Determination of ceftiofur and its metabolite desfuroylceftiofur in bovine serum and milk by ion-paired liquid chromatography

Tyczkowska, Krystyna; Voyksner, Robert D.; Anderson, Kevin L.; Aronson, Arthur L.

doi:10.1016/0378-4347(93)80231-r

Cited by 24 publications

(18 citation statements)

References 5 publications

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“…The comparison of the obtained LOD value (0.31 ng mL 21 ) using the electroanalytical procedure with those obtained by reported method (Tyczkowska et al 1993) (50.00 ng mL 21 ), confirms the higher sensibility of the proposed method. However, analyses of bovine serum sample using the proposed procedure do not require timeconsuming extraction and/or evaporation during the analysis, only separation of the precipitated protein by centrifugation was necessary.…”

Section: Analytical Parameterssupporting

confidence: 91%

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Cathodic Stripping Voltammetry Determination of Ceftiofur Antibiotic in Formulations and Bovine Serum

2006

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A sensitive and reliable stripping voltammetry method was developed to determine the presence of Ceftiofur antibiotic drug. This method is based on the adsorptive accumulation of the drug at a hanging mercury drop electrode and then the initiation of a negative sweep that yielded well-defined cathodic peaks at 20.65 V (1 C) and 21.00 V (2 C) vs. Ag/AgCl reference electrode. To achieve high sensitivity, various experimental and instrumental variables were investigated such as supporting electrolyte, pH, accumulation time and potential, drug concentration, scan rate, convection rate, and working electrode area. The monitored adsorptive current of peak 1 C was directly proportional to the concentration of Ceftiofur; it shows a linear response in the range from 0.50 Â 10 28 to 8.00 Â 10 28 mol L 21 (correlation coefficient ¼ 0.998); and the limit of detection is 6.00 Â 10 210 mol L 21 at an accumulation time of 300 s. The applicability of this approach was illustrated by the determination of Ceftiofur in pharmaceutical preparations and bovine serum.

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Section: Analytical Parameterssupporting

confidence: 91%

“…Barbosa et al 1154 sample bovine serum, based on the average of four determinations, is listed in Table 2. The obtained results were also compared with those obtained by the reported method (Tyczkowska et al 1993). As can be observed, a good agreement between the results was achieved.…”

Section: Analysis Of Bovine Serumsupporting

confidence: 78%

Cathodic Stripping Voltammetry Determination of Ceftiofur Antibiotic in Formulations and Bovine Serum

2006

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“…This versatile technique has been commonly used to analyze cephalosporin residues in biological samples such as milk [2,3], plasma [4], serum [1,2,5], cerebrospinal fluid [5], urine [1,5] and synovial fluid [4]. Some papers have been published the determination of cefoperazone [6] or ceftiofur [4,[7][8][9] in plasma by HPLC and two different plasma pretreatments were used: a simple deproteinization, (using organic solvent or acidic media) [6,7], and a derivatization step followed by SPE [4,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Liquid chromatography (LC) has played a key role in the determination of cephalosporins in pharmaceutical preparations and in biological fluids [2][3][4][5]. This versatile technique has been commonly used to analyze cephalosporin residues in biological samples such as milk [2,3], plasma [4], serum [1,2,5], cerebrospinal fluid [5], urine [1,5] and synovial fluid [4].…”

Section: Introductionmentioning

confidence: 99%

On-line coupling of solid-phase extraction and capillary electrophoresis for the determination of cefoperazone and ceftiofur in plasma

Puig

Tempels

Borrull

et al. 2007

Journal of Chromatography B

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“…HPLC/UV methods exist that provide additional specificity for the detection of this antibiotic. 44,45 However, MS detection methods are needed to confirm the levels of ceftiofur that exceed the set tolerance. Ceftiofur and other antibiotics from the cephalosporin class have been analyzed by LC/electrospray-MS. [45][46][47][48] Based on the presence of a basic nitrogen in ceftiofur and its polar and thermally unstable properties, electrospray positive ion detection (analyzing ceftiofur at low pH to protonate the molecule in solution) should be feasible.…”

mentioning

confidence: 99%

Investigating the use of an octupole ion guide for ion storage and high-pass mass filtering to improve the quantitative performance of electrospray ion trap mass spectrometry

Voyksner

Lee

1999

Rapid Commun. Mass Spectrom.

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An octupole radio frequency (rf) ion guide was evaluated for storage and filtering of ions generated by electrospray prior to introduction into an ion trap mass spectrometer. The control of the rf potential on the ion guide enabled its operation as a high-pass mass filter, removing low-mass chemical noise that would normally fill the ion trap and result in reduced sensitivity, mass resolution, and dynamic range. Also, the ion guide can serve to store the high-pass filtered ions during the ion trap mass analysis, enabling nearly 100% duty cycle and reduction of the cycle time by a factor of two. The linearity, precision, and detection limits of the liquid chromatography (LC) electrospray - ion guide-ion trap MS/MS system were evaluated for the determination of ceftiofur in milk. A linear calibration (linear correlation coefficient of 0.986) from 2-200 ppb was obtained with a relative standard deviation for replicate analysis of less than 8%. A quantitation detection limit of 100 pg of ceftiofur on-column (2 ppb) was achieved from a milk extract. This detection limit is nearly a factor of 10 lower compared with the determination on the same electrospray ion trap system not using a rf ion guide for high-pass mass filtering and ion storage.

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Determination of ceftiofur and its metabolite desfuroylceftiofur in bovine serum and milk by ion-paired liquid chromatography

Cited by 24 publications

References 5 publications

Cathodic Stripping Voltammetry Determination of Ceftiofur Antibiotic in Formulations and Bovine Serum

Cathodic Stripping Voltammetry Determination of Ceftiofur Antibiotic in Formulations and Bovine Serum

On-line coupling of solid-phase extraction and capillary electrophoresis for the determination of cefoperazone and ceftiofur in plasma

Investigating the use of an octupole ion guide for ion storage and high-pass mass filtering to improve the quantitative performance of electrospray ion trap mass spectrometry

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