2021
DOI: 10.2172/1769850
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Determination of Cellulosic Glucan Content in Starch Containing Feedstocks (Laboratory Analytical Procedure)

Abstract: Access to and use of these Methods shall impose the following obligations on the user. The user is granted the right, without any fee or cost, to use, copy, modify, alter, enhance, and distribute these Methods for any purpose whatsoever, except commercial sales, provided that this entire notice appears in all copies of the Methods. The user agrees to credit NREL/Alliance in any publications that result from the use of these Methods. The user also understands that NREL/Alliance is not obligated to provide the u… Show more

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Cited by 5 publications
(3 citation statements)
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“…Then, 84 mL of deionized water was added to the solution immediately, followed by autoclaving at 121 • C for 1 h. Cellulose and hemicellulose were determined from the liquid fraction, which had been neutralized using CaCO 3 that was analyzed using HPLC (Shimadzu, Kyoto, Japan) equipped with an Aminex HPX-87H (Biorad, Hercules, CA, USA) column and a refractive index detector (RID). Lignin was determined from the solid heating fraction at 105 • C for 4 h, then burnt at 575 • C for 2 h [65].…”
Section: Methodsmentioning
confidence: 99%
“…Then, 84 mL of deionized water was added to the solution immediately, followed by autoclaving at 121 • C for 1 h. Cellulose and hemicellulose were determined from the liquid fraction, which had been neutralized using CaCO 3 that was analyzed using HPLC (Shimadzu, Kyoto, Japan) equipped with an Aminex HPX-87H (Biorad, Hercules, CA, USA) column and a refractive index detector (RID). Lignin was determined from the solid heating fraction at 105 • C for 4 h, then burnt at 575 • C for 2 h [65].…”
Section: Methodsmentioning
confidence: 99%
“…The raw materials and residues were characterised according to the NREL laboratory analytical protocol [20,21]. After every technical process, all the samples were centrifuged for 9 min at 3100 rpm, in order to proceed for further chemical analysis.…”
Section: Chemical Analysismentioning
confidence: 99%
“…After every technical process, all the samples were centrifuged for 9 min at 3100 rpm, in order to proceed for further chemical analysis. In the solid fractions, the total solids, volatile solids, moisture, ash, lignin (acid-soluble lignin and acidinsoluble residue), hemicellulose, cellulose [20] and starch [21] were measured. The starch was estimated by the Total Starch Assay Kit, using the AOAC Method 996.11 (K-TSTA-100A, Megazyme, Wicklow, Ireland) [22].…”
Section: Chemical Analysismentioning
confidence: 99%