Determination of cephalosporins utilising thin-layer chromatography with fluorescamine detection

Abstract

A thin-layer chromatographic procedure utilising fluorescamine detection is described for the quantitation of cephradine, cefaclor, cephalexin and cefadroxil. The repeatability, sensitivity and detectability of the method are compared with those for o-phthalaldehyde fluorescence emission and UV absorption measurements on the plate. The applicability of the fluorescamine method to the determination of these cephalosporins in serum, urine and pharmaceutical formulations is discussed.

Development and Validation of the Quantitative Analysis of Ceftazidime in Powder for Injection by Infrared Spectroscopy<SUP>#</SUP>

“…These methods included spectrophotometry [15][16][17][18][19][20][21][22][23][24], high performance liquid chromatography [2,9,[25][26][27][28][29], capillary eletrophoresis [30], fluorimetry [31][32][33][34][35], polarography [36][37][38][39][40] and titrimetry [41].…”

Novel HPLC method for quantitative determination of cefazolin sodium in pharmaceutical formulations

“…Review of the literature shows that considerable attention has been paid to analysis of cefazolin sodium in pharmaceutical preparations, 13,14 urine, [15][16][17] and serum. 18 Conventional methods used for serum drug assays include gas chromatography, 19 polarography, 20 capillary electrophoresis, 21,22 and ultravioletvisible spectrophotometry.…”

Comparison of HPLC and UV Spectrophotometric Methods for the Determination of Cefaclor Monohydrate in Pharmaceutical Dosages

“…Several analytical procedures are available in literature for the analysis of antimicrobial. These methods are spectrophotometry (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13), high performance liquid chromatography (14)(15)(16)(17)(18)(19), capillary electrophoresis (20), fluorimetry (21)(22)(23)(24), polarography (25)(26)(27)(28)(29),titrimetry (30), and bioassay (31)(32). Spectrophotometric assay for determination of other cephalosporins as ceftazidime has been described (33) but no method for Cefaclor monohydrate had been previously described.…”