Determination of Chlorinated Acetic Acids in Drinking Water by In-Situ Derivatization and Solid Phase Microextraction

Aikawa, Bio; Burk, Robert

doi:10.1080/03067319708028364

Cited by 23 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of diethylaminoethanol (DEAE; a component of the G-500 resin), guanidine isothiocyanate (used as chaotropic salt for the DNA-Binding Matrix resin), and CH 3 COONa (a component of SEWS) as contaminants in the extracellular DNA2 was checked by gas chromatographymass spectrometry (GC-MS). The DNA2 extracts were analysed by an Agilent 5971 GC-MS after the following treatments: DEAE was extracted after alkalinisation to pH 11 with dichloromethane (v:v 1:1), guanidine isothiocyanate was derivatised with acetylacetone (Palaitis and Curran 1984), acetate ions were extracted by SPME (solid phase microextraction) after acidification at pH 2 with H 3 PO 4 and derivatised according to Aikawa and Burk (1997) and Godoi et al (2005).…”

Section: Methodsmentioning

confidence: 99%

Purification and isotopic signatures (δ 13C, δ 15N, Δ14C) of soil extracellular DNA

et al. 2007

View full text Add to dashboard Cite

The aim of this work was to obtain pure extracellular DNA molecules so as to estimate their longevity in soil by an isotope-based approach. Extracellular DNA molecules were extracted from all horizons of a forest soil and purified by the procedure of Davis (Purification and precipitation of genomic DNA with phenol-chloroform and ethanol. In: Davis LG, Dibner MD, Battey JF (eds) Basic methods in molecular biology. Appleton & Lange, Norwalk, 16-22, 1986) without (DNA1) or with (DNA2) a successive treatment with binding resins followed by elution. The two differently purified DNA samples were compared for their A 260 /A 280 ratio, polymerase chain reaction (PCR) amplification and natural abundance of stable ( 13 C and 15 N) and radioactive ( 14 C) isotopes. The purity index and the PCR amplification did not differentiate the efficiency of the two purification procedures. The isotopic signature of DNA was more sensitive and was strongly affected by the purification procedures. The isotopic measurements showed that the major contaminant of extracellular DNA1 was the soil organic matter (SOM), even if it is not possible to exclude that the similar δ 13 C, δ 15 N and Δ 14 C values of DNA and SOM could be due to the use of SOM-deriving C and N atoms for the microbial synthesis of DNA. For extracellular DNA2, extremely low values of Δ 14 C were obtained, and this was ascribed to the presence of fossil fuel-derived substances used during the purification, although in amounts not revealed by gas chromatography-mass spectrometry analysis. The fact that it is not possible to obtain contaminant-free DNA molecules and the potential use of soil native organic compounds during the microbial synthesis of DNA make it not achievable to estimate the age of soil extracellular DNA by radiocarbon dating.

show abstract

Section: Methodsmentioning

confidence: 99%

Purification and isotopic signatures (δ 13C, δ 15N, Δ14C) of soil extracellular DNA

et al. 2007

View full text Add to dashboard Cite

show abstract

“…SPME integrates sampling, extraction, concentration and sample introduction into a single step and is therefore an economical and easily automated technique. SPME has been successfully used for the analysis of haloacetic acids, including TCA and DCA, in water, blood or urine 33–35. Dehon et al 36.…”

mentioning

confidence: 99%

In situ derivatization/solid‐phase microextraction coupled with gas chromatography/negative chemical ionization mass spectrometry for the determination of trichloroethylene metabolites in rat blood

Liu

Muralidhara

Bruckner

et al. 2008

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

An in situ derivatization solid-phase microextraction (SPME) method has been developed for the determination of the trichloroethylene (TCE) metabolites, trichloroacetic acid (TCA), dichloroacetic acid (DCA) and trichloroethanol (TCOH), in rat blood. The analytical procedure involves derivatization of TCA and DCA to their ethyl esters with acidic ethanol, headspace sampling using SPME, and gas chromatography/negative chemical ionization mass spectrometry (GC/NCI-MS) determination. Parameters affecting both derivatization efficiency and the headspace SPME procedure, such as the concentration of sulfuric acid, amount of ethanol, derivatization-extraction temperature and time, sample preheating time, agitator speed and desorption conditions, were optimized. The method showed good linearity over the range of 1-1000 ng/mL in rat blood for each metabolite with correlation coefficients (R(2)) higher than 0.99. The intra-day and inter-day precision and accuracy were less than 10%. The relative recoveries for all analytes were greater than 84%. Validation results demonstrated that selected ion monitoring of the (35)Cl and (37)Cl isotopes using NCI resulted in reliable and sensitive quantitation of all three TCE metabolites. This validated method was successfully applied to study the toxicokinetic behavior of TCE metabolites following a 1 mg/kg oral dose of TCE.

show abstract

“…To our knowledge, only two studies report HAAs analysis by SPME. Aikawa and co-workers described the analysis of HAAs in drinking water by in situ acidic derivatization to the methyl esters with HCl/methanol followed by headspace SPME (HS-SPME) and GC-ECD determination using a poly(dimethylsiloxane) fiber. Although this method is rapid, a high limit of detection was obtained for monochloroacetic acid (400 μg/L) and only three chlorinated acetic acids were studied.…”

mentioning

confidence: 99%

In Situ Derivatization/Solid-Phase Microextraction for the Determination of Haloacetic Acids in Water

Santos

2000

Anal. Chem.

View full text Add to dashboard Cite

An in situ derivatization solid-phase microextraction method has been developed for the determination of haloacetic acids (HAAs) in water. The analytical procedure involves derivatization of HAAs to their methyl esters with dimethyl sulfate, headspace sampling using solid-phase microextraction (SPME), and gas chromatography-ion trap mass spectrometry (GC/ITMS) determination. Parameters affecting both derivatization efficiency and head-space SPME procedure, such as the selection of the SPME coating, derivatization-extraction time and temperature, and ionic strength, were optimized. The commercially available Carboxen-poly(dimethylsiloxane) (CAR-PDMS) fiber appears to be the most suitable for the determination of HAAs. Moreover, the formation of HAA methyl esters was dramatically improved (up to 90-fold) by the addition of tetrabutylammonium hydrogen sulfate (4.7 micromol) to the sample as ion-pairing agent in the derivatization step. The precision of the in situ derivatization/HS-SPME/GC/ITMS method evaluated using an internal standard gave relative standard deviations (RSDs) between 6.3 and 11.4%. The method was linear over 2 orders of magnitude, and detection limits were compound-dependent, but ranged from 10 to 450 ng/L. The method was compared with the EPA method 552.2 for the analysis of HAAs in various water samples, and good agreement was obtained. Consequently, in situ derivatization/HS-SPME/GC/ITMS is proposed for the analysis of HAAs in water.

show abstract

Determination of Chlorinated Acetic Acids in Drinking Water by In-Situ Derivatization and Solid Phase Microextraction

Cited by 23 publications

References 6 publications

Purification and isotopic signatures (δ 13C, δ 15N, Δ14C) of soil extracellular DNA

Purification and isotopic signatures (δ 13C, δ 15N, Δ14C) of soil extracellular DNA

In situ derivatization/solid‐phase microextraction coupled with gas chromatography/negative chemical ionization mass spectrometry for the determination of trichloroethylene metabolites in rat blood

In Situ Derivatization/Solid-Phase Microextraction for the Determination of Haloacetic Acids in Water

Contact Info

Product

Resources

About