Determination of chlorogenic acid and rutin in cigarettes by an improved capillary electrophoresis indirect chemiluminescence system

Jiang, Huili; Yang, He; Zhao, Huazhang; Hu, Yan‐Yun

doi:10.1016/j.aca.2004.02.021

Cited by 49 publications

(22 citation statements)

References 43 publications

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“…To date, several analytical methods for the analysis of polyphenols in tobacco have been reported by means of spectrophotometry [6], gas chromatography (GC) or GC-MS [7], high-performance liquid chromatography (HPLC) with chemiluminescence detection [8], ultraviolet detection (UVD) [9,10] and MS detection [11], and capillary electrophoresis (CE) method [12]. Among these methods, spectrophotometry is only used for the determination of the total polyphenols, GC and HPLC are the most powerful.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Analysis of Eight Polyphenols in Tobacco by Rapid Resolution Liquid Chromatogarphy

Xie¹,

Yu²,

Hou³

et al. 2011

AJAC

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A rapid resolution liquid chromatographic (RRLC) method for the determination of eight polyphenols in tobacco was developed. Polyphenols were extracted from tobacco samples by methanol/ water in an ultrasonic bath, then subjected to clean up by solid phase extraction. The separation was performed on a 50 × 4.6 mm, 1.8 μm ZORBAX Eclipse XDB-C18 column. Compared with conventional HPLC method, the analysis time for eight polyphenols by RRLC method was reduced from 20 to 5 min without sacrificing resolution, and the sensitivity was improved. This method appears simple, accurate and precious. The relative standard deviations (RSD) of overall analysis procedure for eight tobacco polyphenols were less than 2% with the recoveries ranging from 94% to 107%. This method could be applied to the rapid determination of major polyphenols in tobacco with satisfactory results.

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Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Analysis of Eight Polyphenols in Tobacco by Rapid Resolution Liquid Chromatogarphy

Xie¹,

Yu²,

Hou³

et al. 2011

AJAC

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“…Even some analytes, such as rutin or chlorogenic acid, can have both effects depending on the experimental conditions. Jiang et al developed a method to determine chlorogenic acid and rutin in cigarettes by indirect CL [88]. Better sensitivity was achieved by Han [89], using a direct method to determine these two compounds in cigarettes and pharmaceutical formulations.…”

Section: Clinical and Pharmaceutical Applicationsmentioning

confidence: 99%

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

2010

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Advances and analytical applications in chemiluminescence coupled to capillary electrophoresisThe present review presents the state of the art of the developments, key strategies and analytical applications of chemiluminescence detection coupled to CE (CE-CL). Different parts considering the most common CL systems have been included, such as the tris(2,2 0 -bipyridine)ruthenium(II) system, the luminol and derivatives reaction, the peroxyoxalate CL or direct oxidations. New advances in homemade configurations and applications in different fields such as clinical, pharmaceutical, environmental and food analysis have been included. The focus is on studies which appeared from 2000 to the end of 2009.

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“…However, analysis of these active ingredients is a challenging task, because they are complex and usually occur as mixture in medicinal plants.HPLC and CE have been employed in the determination of polyphenols with different detectors, such as UV-visible spectrometry (10-13), electrochemistry (14) and indirect CL (15,16). For the CL methods, several CL reagents, such as luminol, acridinium and peroxyoxalate have been utilized.…”

mentioning

confidence: 99%

Chemiluminescence determination of pharmacologically active compounds by capillary electrophoresis

Han

2005

Luminescence

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A simple and rapid capillary electrophoresis with direct chemiluminescence method has been developed for the determination of five natural pharmacologically active compounds including rutin, protocatechuic aldehyde, chlorogenic acid, luteolin and protocatechuic acid. The luminol as a component of the separation electrolyte buffer was introduced at the head of the separation capillary. The separation of five compounds was carried out in a fused-silica capillary with 15.0 mmol/L tetraborate, 1.0 mmol/L SDS and 0.42 mmol/L luminol (pH 8.5). The analytes was determined by enhancing the chemiluminescence of luminol with 0.13 mmol/L K3Fe(CN)6 in 0.05 mol/L NaOH, which was introduced at the post-column stage. The voltage applied was 16 kV. Under the optimum conditions, the analytes were separated within 10 min. The excellent linearity was obtained over two to three orders of magnitude with a detection limit (signal:noise = 3) of 0.012-0.055 micromol/L for all five analytes. The method was successfully used in the analysis of pharmaceutical and biological samples, and the assay results were satisfactory.

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Determination of chlorogenic acid and rutin in cigarettes by an improved capillary electrophoresis indirect chemiluminescence system

Cited by 49 publications

References 43 publications

Rapid and Sensitive Analysis of Eight Polyphenols in Tobacco by Rapid Resolution Liquid Chromatogarphy

Rapid and Sensitive Analysis of Eight Polyphenols in Tobacco by Rapid Resolution Liquid Chromatogarphy

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

Chemiluminescence determination of pharmacologically active compounds by capillary electrophoresis

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