Determination of clenbuterol in porcine tissues using solid-phase extraction combined with ultrasound-assisted dispersive liquid–liquid microextraction and HPLC–UV detection

Liu, Baomi; Yan, Hongyuan; Qiao, Fang; Geng, Yuru

doi:10.1016/j.jchromb.2010.11.017

Cited by 94 publications

(32 citation statements)

References 40 publications

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“…However, in the present study, the totality of tissue supernatant was dried by nitrogen evaporation to increase the signal of these 3 β-agonists, and the detection system was changed to MS/MS. The extraction of β-agonists from meat and other tissues has usually been accomplished by SPE using different reversed-phase adsorbents (Liu et al 2011), but it was indicated in several studies that SPE to separate clenbuterol and other β-agonists from bovine liver resulted in low extraction efficiency.…”

Section: Discussionmentioning

confidence: 99%

“…The chromatographic method that has been published for the extraction and determination of β-agonists in liver using large volumes of solvents is expensive (Liu et al 2011). Meanwhile, compared to gas chromatography (GC) methods that are used for the determination of β-adrenergics, the major advantages of the present method were the short extraction time and no need of a derivatization step.…”

Section: Discussionmentioning

confidence: 99%

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Simultaneous determination of β-agonists and monitoring in bovine tissues by liquid chromatography-tandem mass spectrometry

et al. 2018

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The misuse of β-agonists leads to a potential risk to public health and is forbidden in many countries. We developed a rapid, sensitive and reliable multi-residue detection method for zilpaterol, ractopamine, and clenbuterol in bovine tissues by liquid chromatography-tandem mass spectrometry. Residues were extracted in ethyl acetate after protein precipitation, and then analyzed by the developed method. Good linearities (R 2 > 0.99) were observed, and the recoveries of zilpaterol, ractopamine, and clenbuterol were 99%, 74%, and 102%, respectively. The limits of quantitation for zilpaterol, ractopamine, and clenbuterol were 1.3, 5.0, and 1.7 ng/g, respectively. The method is also applied successfully to bovine tissues within the Korean National Residue Programme. None of the 3 β-agonists were detected from 50 domestic samples. However, zilpaterol (6.3 ng/g) was quantified in one out of the 50 imported samples. The application of this method will be helpful in quality control analysis of β-agonists residues in bovine tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Simultaneous determination of β-agonists and monitoring in bovine tissues by liquid chromatography-tandem mass spectrometry

et al. 2018

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“…UV at fixed wavelength was used for determination of a grand variety of organic compound such as chlorophenols [65], drugs [66,71,77,82,84], insecticides [69,70], aromatic amines [72,73], pesticides [74,75], UV filters [81], additive flame retardant [64,67,68,85], antioxidants [86], etc.…”

Section: Liquid Chromatographymentioning

confidence: 99%

Separation and Preconcentration by Dispersive Liquid–Liquid Microextraction Procedure: Recent Applications

Ojeda

Rojas

2011

Chromatographia

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“…Moreover, the applications of DLLME were limited to water samples but cannot satisfy the requirement of the ultra trace analysis in complex biological matrices. Methods about DLLME combined with SPE to improve the selectivity of the sample preparation process and to result in a very high enrichment factor for complex matrices have been reported [19,[34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Development, validation, and application of a liquid chromatography–tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair

Yang

Guan

et al. 2012

Anal Bioanal Chem

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The tobacco-specific nitrosamine metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) is a valuable biomarker for human exposure to the carcinogenic nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in tobacco and tobacco smoke. In this work, an efficient and sensitive method for the analysis of NNAL in human hair was developed and validated. The hair sample was extracted by NaOH solution digestion, purified by C 18 solid-phase extraction (SPE) and molecularly imprinted solid-phase extraction, further enriched by reverse-phase ultrasound-assisted dispersive liquid-liquid microextraction (USA-DLLME) into 1.0 % aqueous formic acid, and finally analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry. Good linearity was obtained in the range of 0.24-10.0 pg/mg hair with a correlation coefficient of 0.9982, when 150 mg hair was analyzed. The limit of detection and lower limit of quantification were 0.08 and 0.24 pg/mg hair, respectively. Accuracies determined from hair samples spiked with three different levels of NNAL ranged between 87.3 and 107.7 %. Intra-and inter-day relative standard deviations varied from 4.1 to 8.5 % and from 6.9 to 11.3 %, respectively. Under the optimized conditions, an enrichment factor of 20 was obtained. Finally, the developed method was applied for the analysis of NNAL in smokers' hair. The proposed sample preparation procedure combining selectivity of two-step SPE and enrichment of DLLME significantly improves the purification and enrichment of the analyte and should be useful to analyze NNAL in hair samples for cancer risk evaluation and cancer prevention in relation to exposure to the tobaccospecific carcinogen NNK.

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Determination of clenbuterol in porcine tissues using solid-phase extraction combined with ultrasound-assisted dispersive liquid–liquid microextraction and HPLC–UV detection

Cited by 94 publications

References 40 publications

Simultaneous determination of β-agonists and monitoring in bovine tissues by liquid chromatography-tandem mass spectrometry

Simultaneous determination of β-agonists and monitoring in bovine tissues by liquid chromatography-tandem mass spectrometry

Separation and Preconcentration by Dispersive Liquid–Liquid Microextraction Procedure: Recent Applications

Development, validation, and application of a liquid chromatography–tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair

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