Determination of clotrimazole in mice plasma by capillary electrophoresis

Wienen, Frank; Laug, Stefanie; Baumann, Knut; Schwab, Albrecht; Just, Stefan; Holzgrabe, Ulrike

doi:10.1016/s0731-7085(02)00531-9

Cited by 11 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26 Determination of KET and CLO are usually accomplished by chromatographic techniques but strongly needs a prior sample treatment and enrichment step that has been carried out by liquid-liquid extraction (LLE). [27][28][29][30] There has been no report on the separation and determination of KET and CLO in cow's milk. In order to ensure human food safety, the European Union has set maximum residue limits (MRLs) for some antibiotics, such as fenbendazol and enrofloxacin in milk; however, no limit has been ruled out for CLO and KET until now.…”

Section: Resultsmentioning

confidence: 99%

“…Solid phase extraction (SPE) is a relatively rapid, easy and inexpensive method of extraction which has been shown to be applicable in extraction of drug residues from biological matrices, including fatty matrices, e.g., milk. 9 Several SPE methods for extraction of azole antifungal drugs from plasma, serum and biological samples were reported; 10,11 however, no report on application of SPE for separation of antifungal drug residues from dairy products appeared in the literature to the best of the authors' knowledge. Extraction of CLO residue from environmental samples, i.e., water, is reported by Paull and co-workers, 12 using an optimized SPE procedure which was subsequently analyzed by HPLC using a monolithic column.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

SPE-HPLC method for determination of ketoconazole and clotrimazole residues in cow's milk

Beigi¹,

Imani²,

Payehghadr³

et al. 2011

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Um método simples confirmatório para determinação em HPLC-UV de resíduos de cetoconazol e clotrimazol em leite de vaca, após extração da fase sólida (SPE), é relatado neste artigo. Foram removidos das amostras proteínas e lipídeos por tratamento com acetonitrila e conseqüente extração em n-hexano. Os extratos foram posteriormente limpos e concentrados através de extração em fase sólida. Os analitos foram determinados quantitativamente usando um método validado de cromatografia líquida de alta eficiência. O método foi linear no intervalo de 0,1 a 1,0 µg mL -1 para ambos analitos. Os limites de detecção e quantificação para os analitos foram iguais a 0,01 e 0,1 µg mL -1 , respectivamente. Parâmetros importantes que influenciam a eficiência da extração foram investigados e otimizados. O método proposto foi aplicado à análise de amostras de leite. Foram obtidas recuperações satisfatórias no intervalo de 95,9 a 101,78%, usando SPE.A simple confirmatory method for HPLC-UV determination of ketoconazole and clotrimazole residues in cow's milk after solid phase extraction (SPE) is reported in this article. The samples were deprived of proteins and lipids by treating with acetonitrile and consequent extraction in n-hexane. Extracts were further cleaned-up and concentrated via solid phase extraction. The analytes were determined quantitatively using a validated high performance liquid chromatography method. The method was linear in the range of 0.1-1.0 µg mL -1 for both analytes. Limits of detection and quantification for both analytes were equal to 0.01 and 0.1 µg mL -1 , respectively. Important parameters influencing the extraction efficiency were investigated and then optimized; the proposed method was applied to the analysis of milk samples. Satisfactory recoveries were obtained in the range of 95.9-101.78% using SPE.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

SPE-HPLC method for determination of ketoconazole and clotrimazole residues in cow's milk

Beigi¹,

Imani²,

Payehghadr³

et al. 2011

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…The results obtained indicate that the proposed method is a simple, inexpensive and relatively fast extraction technique with little consumption of organic solvent for the extraction and determination of the azole drugs in complex matrices. CZE-DAD Plasma c 300 500-10,000 5.0 ----- [24] HF-LPME-GC/FID Water 0. …”

Section: Discussionmentioning

confidence: 99%

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

2011

View full text Add to dashboard Cite

Hollow fiber liquid-phase microextraction (HF-LPME) based on two immiscible organic solvents (n-dodecane as membrane solvent and 22 lL acetonitrile as acceptor phase) was evaluated for extraction and preconcentration of trace amounts of miconazole and clotrimazole as model azole antifungal drugs, prior to determination by gas chromatography (GC). The drugs were extracted from 24 mL sample solution into a thin layer of organic phase (n-dodecane) immobilized in hollow fiber pores, and then back-extracted into approximately 22 lL organic phase (acetonitrile) located in the lumen of the hollow fiber. After extraction, 1 lL acceptor phase was injected into a GC instrument. Under optimum conditions, limits of detection (LODs) ranged from 0.3-6.0 ng mL -1 , linear dynamic range of 1-400 ng mL -1 and relative standard deviations (RSD%) \5% were obtained. The developed method provided preconcentration factors of 639 and 496 for clotrimazole and miconazole, respectively. The applicability of the extraction method to different types of complicated matrices such as milk, plasma and urine samples was investigated. The results obtained results reveal that the proposed method is a good technique for extraction and determination of the target drugs in different matrices.

show abstract

“…In this paper, we developed a CZE method for the analysis of VTG proteins in fish plasma followed rapid membrane chromatographic purification (RMCP). Capillary zone electrophoresis (CZE) is an established technique that has been utilized to the separation of proteins interest in complex biological fluids including plasma in the past decade [17][18][19]. The exceptional power of separation and resolution, rapid analysis time, economy of reagents and miniscule sample requirements has made CZE an attractive method.…”

Section: Introductionmentioning

confidence: 99%

Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis

Song

Shao

et al. 2005

Journal of Chromatography B

View full text Add to dashboard Cite

Determination of clotrimazole in mice plasma by capillary electrophoresis

Cited by 11 publications

References 20 publications

SPE-HPLC method for determination of ketoconazole and clotrimazole residues in cow's milk

SPE-HPLC method for determination of ketoconazole and clotrimazole residues in cow's milk

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis

Contact Info

Product

Resources

About