Determination of co-administrated opioids and benzodiazepines in urine using column-switching solid-phase extraction and liquid chromatography–tandem mass spectrometry

Fast, Sensitive, and Quantitative Point-of-Care Platform for the Assessment of Drugs of Abuse in Urine, Serum, and Whole Blood

“…Urine drug quantitation, though difficult due to the inter- and intra-individual variability in urine volume and drug excretion/renal function, is still an attractive approach worthy of further development. 42 The rationale is: 1) Urine is usually the preferred matrix in the clinic for determining the presence or absence of drugs because it has a 1- to 3-day window of detection for most drugs and/or their metabolites and is currently the most extensively validated biological specimen for drug testing. 7 Therefore, while drug testing may be performed by either testing urine, serum, oral fluids, sweat or hair, urine drug test (UDT) is predominantly used in the clinic.…”

“…After the test of spiked samples, we moved to test patient urine samples by using the integrated CV-Chip. Urine drug quantitation, though difficult due to the inter- and intraindividual variability in urine volume and drug excretion/renal function, is still an attractive approach worthy of further development . The rationale is as follows: (1) Urine is usually the preferred matrix in the clinic for determining the presence or absence of drugs because it has a 1–3 day window of detection for most drugs and/or their metabolites and is currently the most extensively validated biological specimen for drug testing .…”

Simultaneous Method for the Separation and Identification of Certain Benzodiazepine Drugs in Pharmaceutical Formulations by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

“…Paeng et al, 33 have developed a rapid and simple liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for identification and quantification of BZDs, zolpidem and their metabolites in urine using deuterium labeled internal standards (IS). Xiong et al, 34 have developed a semi-automated method for the determination of four opioids and two-solid-phase extraction (CS-SPE) and LC-MS/MS. A simple and reliable analytical method was established and validated by Jang et al, 35 for the simultaneous determination of twenty-five BZDs and zolpidem in oral fluid obtained using the Quantisal™ collection device.…”

Desenvolvimento de fase monolítica com grupos amino e nano partículas para a determinação de carbidopa, levodopa, benserazida, dopamina e 3-O-metildopa em amostras de plasma de pacientes com a doença de Parkinson por HILIC-MS/MS no modo column switching

“…Sistema column switching em modo foreflush e backflush. Adaptado de(MARCHIONI et al, 2017).Diferentes fases seletivas têm sido utilizadas como coluna 1D no sistema column switching, dentre elas podemos destacar as fases monolíticas (DOMINGUES; SOUZA; QUEIROZ, 2015; MIZUNO; KATAOKA, 2015; LUO; LI; HU, 2017), polímeros molecularmente impressos -MIP -(XU et al, 2010a), materiais de acesso restrito -RAM -(ECKERT;GÖEN, 2014;FAGUNDES et al, 2014;ZHOU et al, 2014;ZHANG et al, 2015; CAGLAR ANDAC, 2016), colunas de fluxo turbulento (CRUTCHFIELD; MARZINKE; CLARKE, HERVIOU et al, 2016;SHIN et al, 2016), imunosorventes (CHAVES; QUEIROZ, 2013), nanotubos de carbono(DE FARIA et al, 2017) e suportes de sílica -C18 -(PARK et al, 2014;PURSLEY et al, 2014;SABAREDZOVIC et al, 2015;XIONG et al, 2015;HÄDENER et al, 2016;ZHONG et al, 2016) A Tabela 2 ilustra algumas aplicações de diferentes fases seletivas na primeira dimensão do sistema LC no modo column switching. Diferentes fases seletivas na primeira dimensão do sistema LC no modo column switching para análise de diferentes analitos em amostras biológicas…”