Determination of collagen type IV by Surface Plasmon Resonance Imaging using a specific biosensor

Sankiewicz, Anna; Łukaszewski, Zenon; Trojanowska, K.; Gorodkiewicz, Ewa

doi:10.1016/j.ab.2016.10.002

Cited by 36 publications

(15 citation statements)

References 20 publications

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“…SPRI measurements were performed as previously described [14,20]. A home-made apparatus and chip architecture were used, as described in Appendix A.2.…”

Section: Spri Measurementsmentioning

confidence: 99%

An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma

Szymańska

Łukaszewski

Żelazowska-Rutkowska

et al. 2021

Sensors

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Human epididymis protein 4 (HE4) is an ovarian cancer marker. Various cut-off values of the marker in blood are recommended, depending on the method used for its determination. An alternative biosensor for HE4 determination in blood plasma has been developed. It consists of rabbit polyclonal antibody against HE4, covalently attached to a gold chip via cysteamine linker. The biosensor is used with the non-fluidic array SPRi technique. The linear range of the analytical signal response was found to be 2–120 pM, and the biosensor can be used for the determination of the HE4 marker in the plasma of both healthy subjects and ovarian cancer patients after suitable dilution with a PBS buffer. Precision (6–10%) and recovery (101.8–103.5%) were found to be acceptable, and the LOD was equal to 2 pM. The biosensor was validated by the parallel determination of a series of plasma samples from ovarian cancer patients using the Elecsys HE4 test and the developed biosensor, with a good agreement of the results (a Pearson coefficient of 0.989). An example of the diagnostic application of the developed biosensor is given—the influence of ovarian tumor resection on the level of HE4 in blood serum.

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“…SPRI measurements were performed as previously described [14,20]. A home-made apparatus and chip architecture were used, as described in Appendix A.2.…”

Section: Spri Measurementsmentioning

confidence: 99%

An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma

Szymańska

Łukaszewski

Żelazowska-Rutkowska

et al. 2021

Sensors

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“…For cancer diagnosis, serum collagen type IV (COLIV), a promising tumor marker overexpressed in the serum of patients with colorectal, gastric, lung, liver and breast cancers, was detected with anti-human collagen type IV antibodies in SPRi, with a high specificity against potential interference substances, including albumin, collagen IV, laminin-5, heparin and glycoprotein GPIIB/IIIA in human samples [126,152]. The toleration in the presence of 100-fold excess of the potential interference substances supported its translational use in clinical cancer diagnosis.…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Recent Advances in Surface Plasmon Resonance Imaging Sensors

Wang

Loo

Chen

et al. 2019

Sensors

116

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The surface plasmon resonance (SPR) sensor is an important tool widely used for studying binding kinetics between biomolecular species. The SPR approach offers unique advantages in light of its real-time and label-free sensing capabilities. Until now, nearly all established SPR instrumentation schemes are based on single- or several-channel configurations. With the emergence of drug screening and investigation of biomolecular interactions on a massive scale these days for finding more effective treatments of diseases, there is a growing demand for the development of high-throughput 2-D SPR sensor arrays based on imaging. The so-called SPR imaging (SPRi) approach has been explored intensively in recent years. This review aims to provide an up-to-date and concise summary of recent advances in SPRi. The specific focuses are on practical instrumentation designs and their respective biosensing applications in relation to molecular sensing, healthcare testing, and environmental screening.

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“…The papers pertaining to stages (iii) and (iv) represent complete analytical procedures [14,15,16,17,18,19,20,21,22,23,24,25,26,27]. These biosensors and related analytical procedures are ready to be subjected to clinical investigation for subsequent use in diagnosis.…”

Section: Stages Of Biosensor Developmentmentioning

confidence: 99%

“…This appears to be a reasonable choice, because determination of the logarithm of the marker concentration does not satisfy expectations for clinical results. An example of an application of straight-line calibration is the determination of collagen IV in the plasma of breast cancer patients [21]. The Langmuirian type calibration contains the initial linear section between 50 and 300 μg L −1 .…”

Section: Calibration Strategymentioning

confidence: 99%

Recent Progress in Surface Plasmon Resonance Biosensors (2016 to Mid-2018)

Gorodkiewicz

Łukaszewski

2018

Biosensors

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More than 50 papers on surface plasmon resonance biosensors, published between 2016 and mid-2018, are reviewed. Papers concerning the determination of large particles such as vesicles, exosomes, cancer cells, living cells, stem cells, and microRNA are excluded, as these are covered by a very recent review. The reviewed papers are categorized into five groups, depending on the degree of maturity of the reported solution; ranging from simple marker detection to clinical application of a previously developed biosensor. Instrumental solutions and details of biosensor construction are analyzed, including the chips, receptors, and linkers used, as well as calibration strategies. Biosensors with a sandwich structure containing different nanoparticles are considered separately, as are SPR (Surface Plasmon Resonance) applications for investigating the interactions of biomolecules. An analysis is also made of the markers determined using the biosensors. In conclusion, there is shown to be a growing number of SPR applications in the solution of real clinical problems.

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Determination of collagen type IV by Surface Plasmon Resonance Imaging using a specific biosensor

Cited by 36 publications

References 20 publications

An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma

An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma

Recent Advances in Surface Plasmon Resonance Imaging Sensors

Recent Progress in Surface Plasmon Resonance Biosensors (2016 to Mid-2018)

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