Determination of Coumarin in Food Using Ultra-Performance Liquid Chromatography–Electrospray-Tandem Mass Spectrometry

Abstract

A method based on the use of ultra performance liquid chromatography (UPLC) tandem mass spectrometry interfaced with electrospray (UPLC=MS=MS) was devised for the determination of coumarin residues in food samples. Sample treatment includes accelerated solvent extraction (ASE) using dichloromethane followed by a clean up on a gel permeation chromatograph. UPLC was performed on an Acquity UPLC BEH C 18 (100 mm  2.1 mm), the mobile phase was water-acetonitrile (50=50 v=v) (each component containing 0.1% formic acid) at a flow rate of 0.3 mL min À1 . For unequivocal identification of the substance, two ions were detected and chosen for multiple reactions monitoring (MRM). Validation was carried out on a spiked sample of vanilla milk rice and liquorice sweets. The described method meets all the criteria of Decision 2002=657=EC and is easy to use in routine analyses.

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

“…Ultra-high-performance liquid chromatography (UHPLC) is another effective tool in overcoming MEs. It is characterized by higher speed, resolution, and sensitivity than normal HPLC [145][146][147][148][149][150]. Despite its high chromatographic performance, the UHPLC has a limit in terms of peak capacity, which, however, can be overcome by developing two-dimensional liquid chromatography techniques (LCxLC, (2D) method) that can resolve samples that current one-dimensional liquid chromatography methods are unable to resolve [151].…”

HPLC separation and determination of dicoumarol and other simple coumarins in sweet clover

“…Table 1 summarizes some studies using HPLC for the determination of investigated coumarins in different matrices. Techniques, such as ultrasonic liquid extraction for plant and cosmetics samples (Muir et al 1992;Ikeda et al 2009;Xi et al 2010) or accelerated solvent extraction for food sample (Vieriková et al 2009) were applied for sample pretreatment. The aim of the present work was to develop a rapid and sensitive HPLC method for simultaneous determination dicoumarol, coumarin, and 4-hydroxycoumarin in sweet clover herb and hay.…”

Qualitative and quantitative determination of coumarin using surface-enhanced Raman spectroscopy coupled with intelligent multivariate analysis

“…10 Thus, analytical methods are needed for the qualitative and quantitative determination of coumarin. Nowadays, several analytical approaches have been used to determine coumarin, such as high performance liquid chromatography (HPLC), [13][14][15] mass spectrometry (MS), 16 ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS), 17,18 liquid chromatography mass spectrometry (LC-MS), 19,20 gas chromatography (GC), 21,22 and gas chromatography with mass spectrometry (GC-MS). 23,24 These methods have high selectivity and sensitivity, but they are complex and time-consuming and require pre-treatment, valuable equipment and good operation skills.…”