Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity activity kinetics and diagnostic significance in myocardial infarction

Neumeier, D.; Prellwitz, W.; Würzburg, U.; Brundobler, Meinhard; Olbermann, M; Just, H; Knedel, M.; Lang, Hermann

doi:10.1016/0009-8981(76)90146-7

Cited by 94 publications

(19 citation statements)

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“…Nach Rekonstitution und chromatographischer Trennung (2) der Homogenate (n = 5) fanden wir für die einzelnen Isoenzyme folgende katalytische Konzentrationen: CK-MM 381,7 ± 19 U/l (Peak 1), CKMB 42 ± 18 U/l (Peak 2), CK-BB 114 ± 25 U/l (Peak 3). Die Bestimmung der Kreatinkinase-B erfolgte mit der von uns mitgeteilten Methode (5,6,8) • --…”

Section: Materials Und Methodenunclassified

Verwendung von Primaten-Gewebshomogenaten zur Qualitätskontrolle der Bestimmung von Kreatinkinase-MB mit inhibierenden Antikörpern

Neumeier¹,

Siegele²,

Glück³

et al. 1977

Clinical Chemistry and Laboratory Medicine

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Zusammenfassung: Nach chromatographischer Trennung der Kreatinkinase-Isoenzyme CK-MM, CK-MB und CK-BB aus Gewebshomogenaten von Rhesusaffen konnte gezeigt werden, daß die Aktivität der Untereinheit CK-M durch inhibitorisches Anti-CK-M-Serum vollständig gehemmt wird. Damit sind Kontrollproben aus Affengewebe prinzipiell zur Qualitätskontrolle der immunologischen Bestimmung von Kreatinkinase-MB geeignet. Präzision in der Serie: n = 56, = 29,2 U/l, s = 3,2 U/l, VK = 11,1 %, Präzision von Tag zu Tag: n = 12, = 166,7 U/l, s = 5,0 U/l, VK = 3,0%. Die Entwicklung von Kontrollseren mit katalytischen Konzentrationen von Kreatinkinase-MB im unteren Konzentrationsbereich ist notwendig. Tlie use of primate tissue homogenates for quality control in the creatine kinase-MB determination with inhibiting antibodiesSummary: The creatine kinase isoenzymes CK-MM, CK-MB and CK-BB fiom Rhesus monkey heart and brain tissue homogenates were separated ehroinatographically. Thereafter it could be demonstrated that the activity of the simian subunit CK-M is completely inhibited by anti-inhibitory-CK-M serum. Thus control sera from simian tissue are in principle suited for quality control in an immunological determination of creatine kinase-MB. The intraassay variance and interassay variance were n = 56, = 29.2 U/l, SD = 3.2 U/l, CV = 11. l % and n = 12, = 166.7 U/l, SD = 5.0 U/l, CV = 3.0% respectively. It is desirable to develop control sera with catalytic concentrations of creatine kinase-MB in a lower range. Einleitung

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Section: Materials Und Methodenunclassified

Verwendung von Primaten-Gewebshomogenaten zur Qualitätskontrolle der Bestimmung von Kreatinkinase-MB mit inhibierenden Antikörpern

Neumeier¹,

Siegele²,

Glück³

et al. 1977

Clinical Chemistry and Laboratory Medicine

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“…Concern with technical aspects of creatine kinase-MB assay has led to modifications of reaction temperature (4,5,13,14), sulfhydryl activators (17,18), inhibitors of adenylate kinase (EC 2.7.4.3) (I.e. (16)) and blanking procedures.…”

Section: Discussionmentioning

confidence: 99%

Aids to the Evaluation of Diagnostic Performance, as Applied to Immunological Creatine-Kinase MB Assay in Myocardial Infarction

Werner¹,

Mohrbacher²

1979

Clinical Chemistry and Laboratory Medicine

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Summary: Immunoassay of creatine kinase-MB provides numerical information, which makes it possible to estimate quantitatively the diagnostic performance following myocardial infarction. Two graphical methods for such an evaluation are presented. The relationship between technical sensitivity and specificity was analyzed using a continuous function, the receiver-operator characteristic curve. Using this function, a diagnostic threshold ("upper normal limit") was chosen, which balances both technical specificity and sensitivity on the first day following infarction. On the second and third days this threshold caused a progressive loss of technical sensitivity. The relationship between effectiveness and the prevalence of myocardial infarction in the tested population was evaluated with a nomogram correlating these two quantities. With the chosen diagnostic threshold, effectiveness is independent of prevalence on the first day, but the loss in technical sensitivity on subsequent days causes effectiveness to decay when the prevalence is high. Bewertungsmaße der diagnostischen Leistungsfähigkeit, angewendet auf die immunologische Kreatinkinase-MB-Bestimmung bei HerzinfarktZusammenfassung: Die immunologische Bestimmung von Kreatinkinase-MB verschafft zahlenmäßige Ergebnisse, welche die Anwendung quantitativer Bewertungsmaße der diagnostischen Leistungsfähigkeit nach Herzinfarkt gestattet. Zwei Mittel für eine solche Wertbestimmung werden dargestellt. Die Wechselbeziehungen zwischen technischer Empfindlichkeit und Spezifität wurden mittels einer kontinuierlichen Funktion, der "Receiver-Operator Characteristic Curve" analysiert. Ein diagnostischer Schwellenwert ("obere Normalgrenze") wurde gewählt, bei welchem sowohl die technische Empfindlichkeit wie die technische Spezifität am ersten Tag nach Infarkt im Gleichgewicht sind. Am zweiten und dritten Tag verursacht dieser Schwellenwert einen zunehmenden Verlust an technischer Empfindlichkeit. Die Beziehung der Effektivität (effectiveness) der Untersuchung zur Häufigkeit Von Herzinfarkt in der untersuchten Population wurde mit Hilfe eines Nomogramms bewertet, welches diese zwei Kenngrößen miteinander korreliert. Mit dem gewählten Schwellenwert ist die Effektivität der Untersuchung am ersten Tag unabhängig von der Krankheitshäufigkeit, aber der Verlust an technischer Empfindlichkeit verursacht bei hoher Krankheitshäufig-keit in den folgenden Tagen eine Verminderung der Effektivität.

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“…Patients with coagulation disorders, collagen vascular disease, thyroid disorder, valvular heart disease and congenital diseases of heart were excluded from the study. After obtaining the consent from subjects and institutional ethical committee clearance, overnight fasting blood samples collected in vacutainers under sterile measures were analyzed for different biochemical parameters like blood sugar levels (FBS and PPBS by GOD-POD method), [14] lipid profile (cholesterol, [15,16] triglycerides, [17,18,19] HDL-C [20,21,22] by precipitation method, LDL-C by calculation method), cardiac enzymes (CK-MB [23] immune inhibition test, LDH [24] and SGOT [25] in the clinical biochemistry laboratory.…”

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Evaluation of Coronary Risk Factors in Patients With Acute Myocardial Infarction

Ramakrishnan¹,

Rangaswamy²

2015

jemds

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INTRODUCTION:Cardiovascular disease is the commonest cause of death globally. Acute myocardial infarction generally occurs when coronary blood flow decreases abruptly after thrombotic occlusion of a coronary artery causing focal or massive necrosis of cardiac muscle. The risk factor concept implies that a person with one risk factor is more likely to develop clinical atherosclerotic event and is more likely to do so earlier than a person with no risk factors. The presence of multiple risk factors further accelerates the atherosclerosis. Hence it is important to identify the major risk factors of coronary atherosclerosis in an individual with acute myocardial infarction so that further preventive measures can be taken in the form of lifestyle modification and pharmacotherapy. MATERIALS AND METHODOLOGY: This is a hospital based study. This study comprises of 100 cases of acute myocardial infarction admitted in ICCU under the department of medicine and 100 normal healthy controls in the age group of 29-85 years. Patients with the evidence of acute MI were diagnosed according to WHO criteria. Blood samples collected in vacutainers were analyzed for different biochemical parameters in the clinical biochemistry laboratory. RESULTS: Common risk factors have been evaluated in our study and we found that maximum MI patients were recorded in the age group of 51-60 years, with respect to other risk factors history like sex, majority of patients were males (82%), Sedentary life style (44%), Mixed dietary habits (84%), Family history of IHD (6%), Dyslipidemia and Smoking (46%), Hypertension (31%), Diabetes (37%), Obesity (18%). In our study we found that 81% of the patients of acute MI had multiple risk factors. CONCLUSION: Thus from the study we can conclude that risk factors play a major role in the genesis of coronary heart disease. Modification of these factors by pharmacotherapy, diet, physical exercises and behavioral therapy can improve the prognosis in these patients and also helps in reduction of incidence of CHD. KEYWORDS: Acute MI (acute myocardial infarction), IHD (ischemic heart disease), CHD (coronary heart disease), Diabetes mellitus, Hypertension. INTRODUCTION:Cardiovascular disease is the commonest cause of death globally. Acute myocardial infarction generally occurs when coronary blood flow decreases abruptly after thrombotic occlusion of a coronary artery causing focal or massive necrosis of cardiac muscle. The risk factor concept implies that a person with one risk factor is more likely to develop clinical atherosclerotic event and is more likely to do so earlier than a person with no risk factors. The presence of multiple risk factors further accelerates the atherosclerosis. Hence it is important to identify the major risk factors of coronary atherosclerosis in an individual with acute myocardial infarction so that further preventive measures can be taken in the form of lifestyle modification and pharmacotherapy. Atherosclerosis is a focal intimal disease of arteries ranging in size from aorta ...

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Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity activity kinetics and diagnostic significance in myocardial infarction

Cited by 94 publications

References 13 publications

Verwendung von Primaten-Gewebshomogenaten zur Qualitätskontrolle der Bestimmung von Kreatinkinase-MB mit inhibierenden Antikörpern

Verwendung von Primaten-Gewebshomogenaten zur Qualitätskontrolle der Bestimmung von Kreatinkinase-MB mit inhibierenden Antikörpern

Aids to the Evaluation of Diagnostic Performance, as Applied to Immunological Creatine-Kinase MB Assay in Myocardial Infarction

Evaluation of Coronary Risk Factors in Patients With Acute Myocardial Infarction

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