Determination of Cyanide and Thiocyanate in Sugihiratake Mushroom Using HPLC Method with Fluorometric Detection

Akiyama, Hiroshi; Toida, Toshihiko; Sakai, Shinobu; Amakura, Yoshiaki; Kondo, Kazunari; Sugita-Konishi, Yoshiko; Maitani, Tamio

doi:10.1248/jhs.52.73

Cited by 39 publications

(23 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lethal dose of cyanide in humans has been reported to range from 50-300 mg/kg body weight [Bolhius 1954, Akiyama et al 2006. Surprisingly, the wheat samples obtained from the open market as shown in Table 3, contained signifi cantly higher quantities of cyanide than the HQCF samples despite having higher pH.…”

Section: Resultsmentioning

confidence: 99%

Use of indigenous technology for the production of High Quality Cassava Flour with similar food qualities as wheat flour

Eleazu

Kolawole

2014

Acta Sci Pol Technol Aliment

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Use of indigenous technology for the production of High Quality Cassava Flour with similar food qualities as wheat flour

Eleazu

Kolawole

2014

Acta Sci Pol Technol Aliment

View full text Add to dashboard Cite

“…Although the occurrence of b-hydroxy-A-valine in P. porrigens was reported 6) , little is known about other constituents. In this context, we have been studying the chemical constituents and contaminants of P. porrigens from various viewpoints, and reported the occurrence of cyanide and thiocyanate in wild P. porrigens collected from several districts in Japan 7) . In this study, we investigated the UV-detected constituents of this mushroom using HPLC.…”

Section: Introductionmentioning

confidence: 99%

Characteristic Long-Chain Fatty Acid of Pleurocybella porrigens

Amakura

Kondo

Akiyama

et al. 2006

J. Food Hyg. Soc. Jpn

Self Cite

View full text Add to dashboard Cite

As part of an investigation on the chemical constituents and contaminants of the basidiomycete Pleurocybella porrigens (Japanese name: Sugihiratake), we analyzed the UV-detected constituents of this mushroom using HPLC. One of the major UV peaks detected was isolated and identified as a-eleostearic acid, a long-chain fatty acid with a conjugated triene moiety, based on the results of spectroscopic methods. a-Eleostearic acid was concluded to be a characteristic fatty acid of P. porrigens, because it was not detected in eight other edible mushrooms examined. Free long-chain fatty acids in P. porrigens and other edible mushrooms were analyzed by HPLC after derivatization with acidic 2-nitrophenylhydrazine hydrochloride. Oleic acid was the main fatty acid in P. porrigens, and saturated long-chain fatty acids such as linoleic acid, palmitic acid, and stearic acid, together with a-eleostearic acid, were also detected.

show abstract

“…7) In the course of this work, we isolated and characterized three novel conjugated long-chain fatty acids (compounds 1-3) together with nine known compounds [(8E,10E)-7,12-dioxo-8,10-octadecadienoic acid (ostopanic acid) (4), 8,9) tryptophan, adenine, trans-cinnamic acid, ergosterol, 10) triolein, 11) a-and beleostearic acids, 12,13) and methyl a-eleostearate 14) ] from aqueous methanol extract of P. porrigens. This paper deals with the structural elucidation of these new compounds based on spectroscopic methods.…”

mentioning

confidence: 99%

Conjugated Ketonic Fatty Acids from Pleurocybella porrigens

Amakura¹,

Kondo²,

Akiyama³

et al. 2006

CHEMICAL & PHARMACEUTICAL BULLETIN

Self Cite

View full text Add to dashboard Cite

The fungus Pleurocybella porrigens (Japanese name: Sugihiratake), a basidiomycete of the Tricholomataceae family, is a small, flat, white mushroom. This edible mushroom grows during the late summer and autumn and is native to all districts of Japan including the Tohoku and Hokuriku areas. In the autumn of 2004, several perplexing acute encephalopathy cases were diagnosed in the Tohoku and Hokuriku areas, and the intake of this mushroom was identified as one of the common factors in these cases.2-6) As a first step to clarify the relationship between the acute encephalopathy cases and this mushroom, we conducted an investigation to determine the chemical constituents of P. porrigens. 7) In the course of this work, we isolated and characterized three novel conjugated long-chain fatty acids (compounds 1-3) together with nine known compounds [(8E,10E)-7,12-dioxo-8,10-octadecadienoic acid (ostopanic acid) (4), 8,9) tryptophan, adenine, trans-cinnamic acid, ergosterol, 10) triolein, 11) a-and beleostearic acids, 12,13) and methyl a-eleostearate 14) ] from aqueous methanol extract of P. porrigens. This paper deals with the structural elucidation of these new compounds based on spectroscopic methods. Results and DiscussionA 50% aqueous methanol extract of P. porrigens was fractionated by chromatography on Diaion HP-20 with aqueous MeOH, then each fraction showing similar patterns on HPLC or TLC was combined. Further purification was performed by a combination of chromatography over MCI-gel CHP 20P with a step-wise gradient elution using aqueous MeOH, preparative HPLC, and preparative TLC to afford three novel compounds (1-3) along with nine known constituents.Compound 1 These spectral features along with four unsaturation degrees in the molecular formula and UV data suggested 1 to be a C18 unsaturated oxo fatty acid with linear structure. The 1 H-13 C heteronuclear multiple-bond connectivity (HMBC) spectrum showed long-range correlations as follows: H-2 and C-1, 3, 4; H-8 and C-6, 7, 9; H-10 and C-9, 12; H-11 and C-9, 10, 13; H-12 and C-11, 14; H-13 and C-11, 14; H-14 and C-12; H-15 and C-13, 14, 16, 17; and H-18 and C-16, 17, indicating the locations of a carbonyl carbon at C-9 and a tertiary carbon at C-14. Although the proposed structure (1) has an asymmetric carbon at C-14, 1 was regarded as a racemate Three novel conjugated long-chain fatty acids (1-3) were obtained from aqueous methanol extracts of Pleurocybella porrigens together with nine known constituents including (8E,10E)-7,12-dioxo-8,10-octadecadienoic acid (ostopanic acid) (4). The structures of the new fatty acids were characterized as (14RS)-(10E,12E)-14-hydroxy-9-oxo-10,12-octadecadienoic acid (1), (12RS)-(8E,10E)-12-hydroxy-7-oxo-8,10-octadecadienoic acid (2), and (10E,12E)-9,14-dioxo-10,12-octadecadienoic acid (3) by spectroscopic methods.

show abstract

Determination of Cyanide and Thiocyanate in Sugihiratake Mushroom Using HPLC Method with Fluorometric Detection

Cited by 39 publications

References 18 publications

Use of indigenous technology for the production of High Quality Cassava Flour with similar food qualities as wheat flour

Use of indigenous technology for the production of High Quality Cassava Flour with similar food qualities as wheat flour

Characteristic Long-Chain Fatty Acid of Pleurocybella porrigens

Conjugated Ketonic Fatty Acids from Pleurocybella porrigens

Contact Info

Product

Resources

About