Determination of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria using column liquid chromatography—mass spectrometry

Sugahara, Kazunori; Ohta, Jun; Takemura, Megumi; Kodama, Hiroyuki

doi:10.1016/0378-4347(92)80397-9

Cited by 5 publications

(1 citation statement)

References 6 publications

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“…The nebulizer and vaporizer temperatures were 260~ and 390~ respectively. Synthetic samples and urinary samples were analysed with a mobile phase of 100mmol/L ammonium acetate-CH3CN (80:20 v/v) at a flow rate of 0.9ml/min (Kodama et al 1990;Sugahara et al 1992).…”

Section: Instrumentationmentioning

confidence: 99%

Identification ofN‐acetyl‐S‐(3‐oxo‐3‐carboxy‐n‐propyl)cysteine in the urine of a patient with cystathioninuria using LC/APCI‐MS

Zhang¹,

Masuoka

Ubuka

et al. 1995

J of Inher Metab Disea

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A new cystathionine metabolite has been identified in the urine of a patient with cystathioninuria using liquid chromatography-mass spectrometry with an atmospheric pressure chemical ionization interface system (LC/APCI-MS). By this method a very intense quasi-molecular ion was observed as a base peak of synthetic N-acetyl-S-(3-oxo-3-carboxy-n-propyl)cysteine (NAc-OCPC). The quasimolecular ion [M + H]+ of NAc-OCPC observed in the urine of a patient with cystathioninuria was the same as that of the authentic compound (m/z 264). The retention time and Rf value on paper chromatography of the synthetic compound were the same as those of the urinary compound from the patient with cystathioninuria. From these results, this new cystathionine metabolite was identified as N-acetyl-S-(3-oxo-3-carboxy-n-propyl)cysteine.

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Section: Instrumentationmentioning

confidence: 99%