Determination of Cytochrome C with Cellulose – DNA Modified Carbon Paste Electrodes

Abstract

A sensor for cytochrome c (cyt c) was developed using a carbon paste electrode (CPE) modified with cellulose-DNA. Cyt c was adsorbed on the cellulose-DNA modified CPE through the electrostatic interaction between them. Owing to this process, a pair of well-defined peaks appeared at 48 mV/85 mV (E pc /E pa vs. Ag/AgCl). This property of the cellulose-DNA modified CPE was utilized for the analysis of cyt c in a biological sample. The optimum experimental conditions for analysis were investigated and a calibration plot was obtained between 1.0 Â 10 À4 M and 1.0 Â 10 À6 M (AE 5% at n 5) at the optimized condition. The detection limit for cyt c at the optimized experimental condition was 5.0 Â 10 À7 M (S/N 3) for 30 min of deposition time with differential pulse voltammetry (DPV). The real sample analysis was carried out with the standard addition method to evaluate the developed method. The content of cyt c in total proteins of 80.0 mg/mL in rat mitocondria fractions was determined to be 0.12 (AE 0.02) mg/mL.

Simultaneous analysis of nitrate and nitrite in a microfluidic device with a Cu-complex-modified electrode

“…This result might have been due to the high resistance of the CPE when the content of the Cu-MPS was over 30%. A similar performance of the CPE was comparable to that seen previously in reported voltammetric methods for the detection of iodine [15] and cyctrochrome c [16] with modified CPEs. Higher ratios resulted in slight increases in the baseline currents and corresponding noise levels.…”

“…Of them, modified carbon paste electrodes (CPEs) can be prepared readily and their surfaces can be regenerated [15][16][17][18]. Recently, CPEs have been used as detectors for the MCE-EC [19,20].…”

Optimum Experimental Condition for Peroxidase-Based Amperometric Biosensor

“…This might be due to the resistance of the electrodes being higher than that of 5% composition of the HRP. The profile of content ratio dependence on steady-state current is consistent with that of the electrode activity [1][2][3].…”

Selective and sensitive furazolidone biosensor based on DNA-modified TiO 2 -reduced graphene oxide

“…In particular, using grapheme-TiO 2 nanocomposites as a bed exploiting has several benefits such as high conductivity to electron transferring plus more active sites for the immobilization of DNA [36,37]. In addition, carbon paste electrode (CPE) was selected for the preparation of DNA biosensor due to a low background current, a wide potential window, distinct advantage of renewability with simple polishing and a simple fabrication procedure [38]. Also presence of nanocomposites such as graphene oxide and TiO 2 can promote the active sites and the electron transfer reactions [39].…”