Determination of Dasatinib in Human Plasma by Using Liquid Chromatography-Tandem Mass Spectrometry

Abstract

A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine dasatinib (DAS) in human plasma. Protein precipitation procedure was used in sample preparation. The analyte and internal standard (dasatinib-d8 (DASD8) were separated on Waters Atlantis dC18 Column (75 × 4.6 mm, 3.5 µm). Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode. The MRM transitions of m/z 488.1 > 401.1 for DAS and 496.15 > 406.1 for DASD8 were used to quantify. The standard curve was found to be linear in the range of 1-400 ng/mL for dasatinib. The lower limit of quantification (LLOQ), using 200 µL human plasma, was 1 ng/mL. The developed and validated method could be applied to the bioequivalence study in human plasma samples.