A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine dasatinib (DAS) in human plasma. Protein precipitation procedure was used in sample preparation. The analyte and internal standard (dasatinib-d8 (DASD8) were separated on Waters Atlantis dC18 Column (75 × 4.6 mm, 3.5 µm). Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode. The MRM transitions of m/z 488.1 > 401.1 for DAS and 496.15 > 406.1 for DASD8 were used to quantify. The standard curve was found to be linear in the range of 1-400 ng/mL for dasatinib. The lower limit of quantification (LLOQ), using 200 µL human plasma, was 1 ng/mL. The developed and validated method could be applied to the bioequivalence study in human plasma samples.
A simple, rapid and sensitive method was developed and validated using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for determination of Bezafibrate (BEZ) in human plasma. The plasma samples were extracted by protein precipitation using bezafibrate-d4 as Internal Standard (IS). The chromatographic separation was performed on Sunfire C18, 3.5µ, 2.1x50mm with a mobile phase consisting of formic acid, water and acetonitrile at flow rate of 0.30 mL/min. BEZ was detected and identified by mass spectrometry with Electrospray Ionization (ESI) in negative ion and Single Ion Recording (SIR) mode. The method was linear in the range of 100-20000 ng/mL for BEZ. The lower limit of quantification was 100 ng/mL. This method could be applied to bioequivalence studies in human plasma samples.
The outbreak of Covid-19 infection has become a pandemic affecting millions of people throughout the world. No specific antiviral drugs have been approved for the treatment of Covid-19. The antiretroviral drug favipiravir (FAV) has been experimentally used for COVID-19 treatment since March 2020 in Japan. China's Science and Technology Ministry official announced that Japan-based anti-flu drug FAV has been observed to help Covid-19 patients recover. Because of that, a simple LC-MS/MS method for the quantification of FAV in human plasma was developed and validated. Sample preparation was carried out using protein precipitation. The FAV and internal standard ([ 13 C 15 N] Favipiravir, (IS)) were chromatographed under a liquid chromatography tandem mass spectrometry (LC-MS/MS) system on a Shiseido Capcell PAK C18, (250 × 4.6 mm, 5 µm) with mobile phase consisting of water and acetonitrile (15/85, v/v). FAV was analyzed by LC-MS/MS with electrospray ionization in the negative ion mode and the multiple reaction monitoring (MRM) mode. MRM transitions were m/z 156.1 ˃ 113.1 for FAV and m/z 158.1 ˃ 113 for IS. The lower limit of quantitation (LLOQ) for this method is 80 ng/mL. The linear calibration range is 80-30000 ng/mL for FAV. The results of the intra-and inter-day precision and accuracy studies were well within the acceptable limits. The method is precise and sensitive enough for its intended purpose. This validated method was applied to pharmacokinetic studies of favipiravir.
A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine dasatinib (DAS) in human plasma. Protein precipitation procedure was used in sample preparation. The analyte and internal standard (dasatinib-d8 (DASD8) were separated on Waters Atlantis dC18 Column (75 × 4.6 mm, 3.5 µm). Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode. The MRM transitions of m/z 488.1 > 401.1 for DAS and 496.15 > 406.1 for DASD8 were used to quantify. The standard curve was found to be linear in the range of 1-400 ng/mL for dasatinib. The lower limit of quantification (LLOQ), using 200 µL human plasma, was 1 ng/mL. The developed and validated method could be applied to the bioequivalence study in human plasma samples.
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