Determination of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolated from durum wheat in different Italian regions

Salomoni, D.; Bertacchini, E.; Alkadri, Dima; Pisi, Annamaria; Tonti, Stefano; Alberti, I.; Prà, M. Dal; Pancaldi, D.; Covarelli, Lorenzo; Nipoti, Paola

doi:10.2478/v10129-011-0030-1

Cited by 20 publications

(31 citation statements)

References 6 publications

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“…Such a deletion is present in all isolates of F. culmorum tested to date (Chandler et al 2003;Kammoun et al 2010), but it is rather rare in F. graminearum (Lee et al 2001;Chandler et al 2003). Our results are consistent with the chemotype screenings from Eastern Europe, where 15-ADON chemotype dominates (Prodi et al 2009;Talas et al 2011), whereas 3-ADON chemotype is more frequent in Northen Europe (Jestoi et al 2008). The present samples included no NIV producing isolates.…”

Section: Resultssupporting

confidence: 81%

“…The present samples included no NIV producing isolates. Talas et al (2011) also detected only 1.2% NIV producing isolates in neighbouring Germany, although low to medium frequencies of this chemotype have been recorded in Europe (Stepien et al 2008;Audenaert et al 2009;Prodi et al 2009). The analysis of a larger collection of Czech F. graminearum isolates would probably reveal a frequency of NIV producers that comparable to detected in Germany.…”

Section: Resultsmentioning

confidence: 99%

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Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat

Sumíková¹,

Gabrielová²,

Kučera³

et al. 2013

Czech J. Food Sci.

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Sumíková T., Gabrielová L., Kučera L., Žabka M., Chrpová J. (2013): Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat. Czech J. Food Sci., 31: 407-412.Fusarium head blight (FHB) is a serious cereal disease in the Czech Republic. The most important pathogen associated with FBH is Fusarium graminearum, which can produce trichothecenes, mainly deoxynivalenol (DON) and its derivates. A set of 103 F. graminearum isolates were isolated from naturally infected wheat ears collected from 20 localities (25 ears from one locality) within the Czech Republic, in the year 2004. The ears were evaluated for DON content by ELISA. Maximum detected value was 30.7 mg/kg. A group of PCR assays targeting the segments of the Tri7, Tri13 and Tri3 genes were used to determine the chemotypes of F. graminearum isolates. All the isolates belonged to DON producing chemotype. Further discrimination revealed that almost all (99.03%) isolates belonged to 15-ADON chemotype, and only one (0.97%) isolate belonged to 3-ADON chemotype. The genetic variability of the isolates was assessed from their AFLP fingerprints. The populations were highly heterogeneous both within and between locations, and no clear evidence for the association between AFLP profile and geographic origin was found out.

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Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat

Sumíková¹,

Gabrielová²,

Kučera³

et al. 2013

Czech J. Food Sci.

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“…The 3- isolates belonging to population 2 were included in the analysis of aggressiveness, ten of these were included in the growth rate analysis c 17 3-ADON isolates were included in the analysis of aggressiveness, 16 of these were included in the growth rate analysis ADON chemotype also predominated among a small set of FGSC isolates from an earlier collection of Norwegian cereals (Langseth et al 1999(Langseth et al , 2001, and was found in oat and wheat samples collected between 2009 and 2011 in Sweden, whereas the 15-ADON chemotype was not observed (Fredlund et al 2013;Lindblad et al 2013 (Nielsen et al 2012). Our data add to a growing set of studies indicating a geographical partitioning of trichothecene types (chemotypes or genotypes) throughout Europe, with 3-ADON dominating in Northern Europe, whereas 15-ADON appears predominant in the Western and Southern Europe including the UK (Jennings et al 2004), Belgium (Audenaert et al 2009), Italy (Prodi et al 2009;Somma et al 2014), Luxembourg (Pasquali et al 2010), Poland (Stępień et al 2008), Hungary (Toth et al 2005), Germany (Talas et al 2011), and France (Boutigny et al 2014). …”

Section: Discussionmentioning

confidence: 60%

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

et al. 2015

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As has been observed in several European countries, the frequency of Fusarium head blight (FHB) caused by members of the Fusarium graminearum species complex (FGSC) has increased in Norwegian cereals in recent years, resulting in elevated levels of deoxynivalenol in cereal grains. The objective of this study was to determine if this increase was associated with changes in FGSC composition within Norway. FGSC isolates collected from wheat, oats and barley in Norway during two periods, mainly 1993-1998 and 2004-2007, were characterized to determine species and trichothecene genotype composition and to assess levels of genetic variation and population structure. In vitro growth rates at different temperatures and aggressiveness in spring wheat were further characterized for a sub-selection of isolates. All Norwegian isolates were identified as F. graminearum. The 3-acetyl-deoxynivalenol (3-ADON) trichothecene type was dominant. However, isolates with the 15-ADON chemotype were detected in Norway for the first time and may represent a recent introduction of this trichothecene type. Bayesian-model based clustering and analyses of genetic differentiation indicated the persistence over the last 20 years of two sympatric and partially admixed populations of F. graminearum in Norway. Significant differences in average in vitro growth rates and aggressiveness were observed between these two populations. Our results demonstrate that the recent increase in prevalence of the FGSC in Norwegian cereals do not correspond to any dramatic changes in FGSC species or trichothecene chemotype composition. However, significant changes in population frequencies were observed among Norwegian F. graminearum.

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“…different countries and/or continents (Prodi et al, 2009b), but some host preference may affect the composition of chemotypes of F. graminearum population. Within the same country, the different geographical areas (i.e.…”

mentioning

confidence: 99%

“…The potential capacity of F. graminearum isolates to produce trichothecenes was evaluated using a multiplex PCR version (Starkey et al, 2007) as described by Prodi et al (2009b). Primers, designed in the region of the Tri12 gene located in the terminal gene cluster for trichothecene biosynthesis, can distinguish three subgroups depending on the type of β-trichothecene produced.…”

mentioning

confidence: 99%

Difference in Chemotype Composition of Fusarium graminearum Populations Isolated from Durum Wheat in Adjacent Areas Separated by the Apennines in Northern-Central Italy

Purahong¹,

Tonti²,

Salomoni³

et al. 2011

The Plant Pathology Journal

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Chemotype composition of Fusarium graminearum strains, isolated from durum wheat kernels from naturally FHB infected fields in Northern and Central Italy, was investigated by multiplex PCR. The different climatic and environmental conditions of the two examined areas separated by the Apennines affected the composition of chemotypes. 15Ac-DON chemotype was predominant in both the sub areas. Nivalneol chemotype was more frequent in the warmer sub area.

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Determination of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolated from durum wheat in different Italian regions

Cited by 20 publications

References 6 publications

Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat

Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

Difference in Chemotype Composition of Fusarium graminearum Populations Isolated from Durum Wheat in Adjacent Areas Separated by the Apennines in Northern-Central Italy

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