Determination of dexamethasone and dexamethasone sodium phosphate in human plasma and cochlear perilymph by liquid chromatography/tandem mass spectrometry

Mei, Zhang; Moore, Grant A.; Jensen, Berit Packert; Begg, Evan J.; Bird, Philip

doi:10.1016/j.jchromb.2010.11.003

Cited by 37 publications

(28 citation statements)

References 10 publications

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“…Several LC-MS/MS methods for the determination of dexamethasone in plasma have been reported in the literature, which showed obvious disadvantages needing improvement, such as a high LLOQ of 20-500 ng/mL or complicated extraction methods for sample preparation (Coimbra et al, 2012;Difrancesco et al, 2007;Li et al, 2013;Yang et al, 2008;Zhang et al, 2011). Solid-phase extraction and liquid-liquid extraction were applied to further reduce the LLOQ; however, these methods were rather time-consuming and money-expending, which still limited their extensive application (Difrancesco et al, 2007;Yang et al, 2008).…”

Section: Development and Optimization Of The Lc-ms/ms Methodsmentioning

confidence: 99%

“…However, to the best of our knowledge, no analytical method for the determination of dexamethasone in nude mice plasma has been established. In addition, some LC-MS/MS methods previously reported for the determination of dexamethasone in plasma showed a high lower limit of quantitation (LLOQ) of 20-500 ng/mL (Coimbra et al, 2012;Li et al, 2013;Zhang et al, 2011). Recent studies showed that the application of solid-phase extraction and liquid-liquid extraction might further reduce the LLOQ to some extent, but the complicated procedures for sample preparation still limit the widespread use of these methods (Difrancesco et al, 2007;Yang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a highly sensitive LC‐MS/MS method for the determination of dexamethasone in nude mice plasma and its application to a pharmacokinetic study

Yin

Zhou

et al. 2014

Biomedical Chromatography

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In the current study, a simple, sensitive and rapid analytical method for the determination of dexamethasone was developed and applied to a pharmacokinetic study in nude mice. Using testosterone as an internal standard, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach after one-step precipitation with acetonitrile was validated and used to determine the concentrations of dexamethasone in nude mice plasma. The method utilized a simple isocratic reverse phase separation over a Dionex C18 column with a mobile phase composed of acetonitrile-water (40:60, v/v). The analyte was detected by a triple quadrupole tandem mass spectrometer via electrospray and multiple reaction monitoring was employed to select both dexamethasone at m/z 393.0/147.1 and testosterone at m/z 289.5/97.3 in the positive ion mode. The calibration curves were linear (r >0.99) ranging from 2.5 to 500 ng/mL with a lower limit of quantitation of 2.5 ng/mL. The relative standard deviation ranged from 1.69 to 9.22% while the relative error ranged from -1.92 to -8.46%. This method was successfully applied to a preclinical pharmacokinetic study of dexamethasone and its pharmacokinetics was characterized by a two-compartment model with first-order absorption in female nude mice.

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Section: Development and Optimization Of The Lc-ms/ms Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and validation of a highly sensitive LC‐MS/MS method for the determination of dexamethasone in nude mice plasma and its application to a pharmacokinetic study

Yin

Zhou

et al. 2014

Biomedical Chromatography

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“…Verapamil and clozapine were analyzed using an Agilent 1100 LC coupled to an Agilent SL ion trap mass spectrometer for selective ion monitoring of m/z 303 and multiple reaction monitoring of m/z (327 [ 270), respectively (Kollroser & Schober 2002, Sun et al 2004). Fenofibrate, dexamethasone, amiodarone, and carbamazepine were all analyzed using a Waters 2695 LC coupled to a Micromass Quattro Ultima tandem mass spectrometer (Kuhn et al 2010;Vazquez-Roig et al 2010;Zhang et al 2011). The following m/z transitions were analyzed using multiple reaction monitoring: fenofibrate (360 [ 233), dexamethasone (484 [ 95), and carbamazepine (237 [ 194).…”

Section: Methodsmentioning

confidence: 99%

Early Life-Stage Toxicity of Eight Pharmaceuticals to the Fathead Minnow, Pimephales promelas

Overturf

Baxter

et al. 2011

Arch Environ Contam Toxicol

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Human pharmaceuticals are routinely being detected in the environment, and there is growing concern about whether these drugs could elicit effects on aquatic organisms. Regulatory paradigms have shifted accordingly, with a greater emphasis on chronic toxicity data compared with acute data. The Organisation for Economic Co-operation and Development 210 Early Life Stage Test has been proposed as a good measure of the potential for pharmaceuticals to elicit chronic toxicity. To begin building a data set regarding the early life-stage toxicity of pharmaceuticals to fish, fathead minnows (FHM) were exposed to amiodarone, carbamazepine, clozapine, dexamethasone, fenofibrate, ibuprofen, norethindrone, or verapamil. Survival and growth were used to assess chronic toxicity in FHM at 28 days posthatch. Exposure of FHM to carbamazepine, fenofibrate, and ibuprofen resulted in no significant adverse effects at the concentrations tested. FHM survival was not impacted by verapamil exposure; however, growth was significantly decreased at 600 μg/L. Dexamethasone-exposed FHM showed a significant decrease in survival at a concentration of 577 μg/L; however, growth was not impacted at the concentration tested. Norethindrone exposure resulted in a significant decrease in survival and dry weight at 14.8 and 0.74 μg/L, respectively. Exposure to amiodarone and clozapine resulted in a significant decrease in survival and a significant increase in growth at concentrations of 1020 and 30.8 μg/L, respectively. Although the effect levels derived in this study are greater then concentrations observed in the environment, these data suggest that synthetic progestins may require additional research.

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“…The actual concentration inside the perilymph after intratympanic injection, however, was much lower than that of the original solution injected into the middle ear cavity. Experimental data are available for perilymphatic concentrations after the intratympanic injection of dexamethasone (molecular weight: 392.46 g mol −1 ), in which 160-1250-fold dilution from the originally injected solution was reported (Bird, Murray, Zhang, & Begg, 2011;Parnes, Sun, & Freeman, 1999;Zhang, Moore, Jensen, Begg, & Bird, 2011). Only one study estimated the gadolinium concentration inside the perilymph after intratympanic injection (Pyykko, Zou, Poe, Nakashima, & Naganawa, 2010).…”

Section: Figurementioning

confidence: 99%

In vivo assay of the potential gadolinium‐induced toxicity for sensory hair cells using a zebrafish animal model

Rah

Han

Park

et al. 2018

J of Applied Toxicology

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Recently, intratympanic injection of gadolinium-based contrast agent (GdC) is growing in use to visualize the endolymphatic hydrops. Although GdC has been quite safely used over 20 years through intravenous injection, the biological influence of GdC on sensory hair cells needs to be thoroughly assessed for wider clinical application of it through intratympanic injection. In this in vivo experimental study, the summated number of sensory hair cells (SO1, SO2, O1 and OC1 neuromasts) showed a steep decrease in the group exposed to 10% and 20% GdC (35.7 ± 7.3, 15.09 ± 10.82, respectively, P < .01) compared with the control group (47.18 ± 2.30). An increase in apoptosis was also observed in the group exposed to 20% gadolinium (7.20 ± 5.56), as compared with the control group (0.08 ± 0.72) or the group exposed to 10% gadolinium (3.48 ± 3.32). A significant reduction in the viable cytoplasmic mitochondria was observed in embryos exposed to 20% GdC (369 ± 124 μm , P = .01) as compared with control embryos (447 ± 118 μm ) or embryos exposed to 10% GdC (420 ± 108 μm ). GdC administration did not impact peripheral neural structures. GdC caused a significant reduction in sensory hair cell counts in response to high concentrations along with increased apoptosis and mitochondrial damage. However, it may not be likely that GdC will lead to hair cell toxicity, as the estimated concentration in the inner ear after clinically tried intratympanic injection is far more diluted than the non-toxic concentration (0.625%) that was tested in this study.

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Determination of dexamethasone and dexamethasone sodium phosphate in human plasma and cochlear perilymph by liquid chromatography/tandem mass spectrometry

Cited by 37 publications

References 10 publications

Development and validation of a highly sensitive LC‐MS/MS method for the determination of dexamethasone in nude mice plasma and its application to a pharmacokinetic study

Development and validation of a highly sensitive LC‐MS/MS method for the determination of dexamethasone in nude mice plasma and its application to a pharmacokinetic study

Early Life-Stage Toxicity of Eight Pharmaceuticals to the Fathead Minnow, Pimephales promelas

In vivo assay of the potential gadolinium‐induced toxicity for sensory hair cells using a zebrafish animal model

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