Determination of Dissolved CO2 Concentration in Culture Media: Evaluation of pH Value and Mathematical Data

Adnan, Amir Izzuddin; Ong, Mei Yin; Nomanbhay, Saifuddin; Show, Pau Loke

doi:10.3390/pr8111373

Cited by 12 publications

(6 citation statements)

References 25 publications

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“…We speculate that the high temperatures reached in the autoclave process increase the conversion rate of dissolved NH 4 + to ammonia gas (NH 3(g) ). When temperature rises, CO 2 solubility in wastewater decreases causing CO 2(g) to be expelled leaving a more alkaline pH ( Adnan et al, 2020 ). Under these physicochemical conditions, the disassociation of NH 4 + ions is increased, and the equilibrium between NH 3 liquid and gas phase shifts towards generating NH 3(g) that leaves the wastewater sample ( Mohammed-Nour et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Native microalgal-bacterial consortia from the Ecuadorian Amazon region: an alternative to domestic wastewater treatment

López-Patiño,

Cárdenas-Orrego,

Torres

et al. 2024

Front. Bioeng. Biotechnol.

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In low-middle income countries (LMIC), wastewater treatment using native microalgal-bacterial consortia has emerged as a cost-effective and technologically-accessible remediation strategy. This study evaluated the effectiveness of six microalgal-bacterial consortia (MBC) from the Ecuadorian Amazon in removing organic matter and nutrients from non-sterilized domestic wastewater (NSWW) and sterilized domestic wastewater (SWW) samples. Microalgal-bacterial consortia growth, in NSWW was, on average, six times higher than in SWW. Removal rates (RR) for NH4+- N and PO43−-P were also higher in NSWW, averaging 8.04 ± 1.07 and 6.27 ± 0.66 mg L−1 d−1, respectively. However, the RR for NO3− -N did not significantly differ between SWW and NSWW, and the RR for soluble COD slightly decreased under non-sterilized conditions (NSWW). Our results also show that NSWW and SWW samples were statistically different with respect to their nutrient concentration (NH4+-N and PO43−-P), organic matter content (total and soluble COD and BOD5), and physical-chemical parameters (pH, T, and EC). The enhanced growth performance of MBC in NSWW can be plausibly attributed to differences in nutrient and organic matter composition between NSWW and SWW. Additionally, a potential synergy between the autochthonous consortia present in NSWW and the native microalgal-bacterial consortia may contribute to this efficiency, contrasting with SWW where no active autochthonous consortia were observed. Finally, we also show that MBC from different localities exhibit clear differences in their ability to remove organic matter and nutrients from NSWW and SWW. Future research should focus on elucidating the taxonomic and functional profiles of microbial communities within the consortia, paving the way for a more comprehensive understanding of their potential applications in sustainable wastewater management.

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Section: Resultsmentioning

confidence: 99%

Native microalgal-bacterial consortia from the Ecuadorian Amazon region: an alternative to domestic wastewater treatment

López-Patiño,

Cárdenas-Orrego,

Torres

et al. 2024

Front. Bioeng. Biotechnol.

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“…Then, the k L a was determined in the 80 L plastic bag, operated under the same conditions mentioned for the 16 L culture, but with different airflows (50, 70, and 80 L min −1 ). The main reason is that the solubility of CO 2 in aquatic environments varies significantly and depends on pH, salinity, pressure, and temperature, i.e., CO 2 solubility in water decreases with decreasing pH [ 28 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Implementation of kLa-Based Strategy for Scaling Up Porphyridium purpureum (Red Marine Microalga) to Produce High-Value Phycoerythrin, Fatty Acids, and Proteins

et al. 2021

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Porphyridium purpureum is a well-known Rhodophyta that recently has attracted enormous attention because of its capacity to produce many high-value metabolites such as the pigment phycoerythrin and several high-value fatty acids. Phycoerythrin is a fluorescent red protein-pigment commercially relevant with antioxidant, antimicrobial activity, and fluorescent properties. The volumetric mass transfer coefficient (kLa) was kept constant within the different scaling-up stages in the present study. This scaling-up strategy was sought to maintain phycoerythrin production and other high-value metabolites by Porphyridium purpureum, using hanging-bag photobioreactors. The kLa was monitored to ensure the appropriate mixing and CO2 diffusion in the entire culture during the scaling process (16, 80, and 400 L). Then, biomass concentration, proteins, fatty acids, carbohydrates, and phycoerythrin were determined in each step of the scaling-up process. The kLa at 16 L reached a level of 0.0052 s−1, while at 80 L, a value of 0.0024 s−1 was achieved. This work result indicated that at 400 L, 1.22 g L−1 of biomass was obtained, and total carbohydrates (117.24 mg L−1), proteins (240.63 mg L−1), and lipids (17.75% DW) were accumulated. Regarding fatty acids production, 46.03% palmitic, 8.03% linoleic, 22.67% arachidonic, and 2.55% eicosapentaenoic acid were identified, principally. The phycoerythrin production was 20.88 mg L−1 with a purity of 2.75, making it viable for food-related applications. The results of these experiments provide insight into the high-scale production of phycoerythrin via the cultivation of P. purpureum in an inexpensive and straightforward culture system.

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“…pH value in the river is influenced by the decomposition process, industrial pollutants, and acidic runoff water [17] . The addition of CO 2 into the water could increase H 2 CO 3 , then decreases the pH value of the water [18] . Station 3 has the lowest pH value, this is due to the high amount of industrial waste disposal in station 3.…”

Section: Physical and Chemical Parameters Of Upstream Citarum River Watermentioning

confidence: 99%

Water pollution level of the Upstream Citarum river based on macrozoobenthic family biotic index

Anwar¹,

Dhahiyat²,

Zahidah³

et al. 2021

Int. J. Fish. Aquat. Stud.

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Citarum River was known as the dirtiest river in the world in 2009, it was caused by the increase in population growth around the Citarum River, which damaging the river and the surrounding environment. This research was conducted at 4 stations, namely 1.Situ Cisanti; 2.Wangisagara; 3.Majalaya; and 4.Sapan, by using purposive sampling method and descriptive quantitative analysis method to determine the level of water pollution in the Upstream Citarum River based on the family biotic index of macrozoobenthos. The results showed that the level of water pollution in the Upstream Citarum River was light to severe. The FBI value of the Upstream Citarum River ranges from 3,95-5,85. Station 1 and station 2 have slightly bad water criteria with each FBI value is 5,85 and 5,77, station 3 has an FBI value of 3,95 with very good water criteria, and station 4 has an FBI value of 5,63 with sufficient water criteria.

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Determination of Dissolved CO2 Concentration in Culture Media: Evaluation of pH Value and Mathematical Data

Cited by 12 publications

References 25 publications

Native microalgal-bacterial consortia from the Ecuadorian Amazon region: an alternative to domestic wastewater treatment

Native microalgal-bacterial consortia from the Ecuadorian Amazon region: an alternative to domestic wastewater treatment

Implementation of kLa-Based Strategy for Scaling Up Porphyridium purpureum (Red Marine Microalga) to Produce High-Value Phycoerythrin, Fatty Acids, and Proteins

Water pollution level of the Upstream Citarum river based on macrozoobenthic family biotic index

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