Determination of drugs in untreated body fluids by micellar chromatography with fluorescence detection

A concise review of applications of micellar liquid chromatography to study biologically active compounds

“…The MLC, being a kind of classical liquid chromatography in the reversed‐phase system, is recognized as a very effective analytical method to study biologically active compounds, which has been confirmed by numerous papers and scientific dissertations. The first reports about possible application of micellar chromatography in analytical studies appeared in 1983–1984 (Mullins and Kirkbright, ; Kirkbright and Mullins, ; Barford and Śliwiński, ; Landy and Dorsey, ) and in 1985 its application in the analysis of biologically active compounds present in body fluids was highlighted (Arunyanart and Cline‐Love, ). Rapid growth of researchers' interest in various applications of MLC, particularly in the determination of drugs and pharmaceutical preparations in body fluids was observed in the 1990s.…”

“…However, one of the most significant and unique advantages of micellar chromatography is the possibility of direct injection of body fluids to the chromatographic system (Arunyanart and Cline‐Love, ; Perez‐Martinez et al ., ; Rambla‐Alegre et al ., ; Garcia‐Alvarez‐Coque and Carda‐Broch, ; Martin‐Biosca et al ., ; Capella‐Peiro et al ., 2002a, b; Reynolds and Albazi, ; Monferrer‐Pons et al ., ; Chen and Wang, ; Dorsey, ; Cline‐Love et al ., ). Owing to the capability of solubilization of various compounds by the micelles present in the mobile phase, matrices of biological samples are washed out with the front of the eluent, which allows a shorter time of analysis of biological‐origin samples.…”

Study of influence of additives of tyloxapol on the chromatographic characteristics of the model compounds: the comparative characterization of micellar mobile phases of tyloxapol and Triton X‐100

“…One of the main appeals of MLC is the possibility of determining drugs in physiological fluids without the need for previous separation of the components presented in these samples. As a result the duration of sample analysis decreases significantly (DeLuccia et al, 1985;Arunyanart and Cline Love, 1985).…”

Separation of related opiate compounds using capillary electrochromatography

“…Therefore, screening and confirmation of forensic drugs is important for the investigation of potential users of drugs and the control of drug addicts following withdrawal therapy. These drugs are usually screened by gas chromatography-mass spectrometry (GC-MS) [2,3], high performance liquid chromatography (HPLC) [4,5], thinlayer chromatography (TLC) [6,7], and, to a lesser extent, by supercritical fluid chromatography (SFC) [8]. On-line coupled LC-GC has been applied in the determination of heroin and its metabolites in urine [9].…”