Determination of Dry Mass, Thickness, Solid and Water Concentration in Living Cells

Barer, R.

doi:10.1038/1721097a0

Cited by 172 publications

(124 citation statements)

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“…( b ) Representative specific refractive index increments of selected biomolecule groups, with the averages of these selected values indicated as horizontal bars. Data are shown for whole-cell average values 73,74 , proteins (multiprotein average 75–78 , bovine serum albumin (BSA) 79 and Evans bacteriological peptone 79 ), nucleic acids (DNA 80–82 and RNA 83,84 ) and other components (CaCl 2 85 , NaCl 85 , KCl 85 , sucrose 85 and dipalmitoyl phosphatidylcholine (DPPC), a common phospolipid 86 ).…”

Section: Figurementioning

confidence: 99%

Live-cell mass profiling: an emerging approach in quantitative biophysics

2014

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Cell mass, volume and growth rate are tightly controlled biophysical parameters in cellular development and homeostasis, and pathological cell growth defines cancer in metazoans. The first measurements of cell mass were made in the 1950s, but only recently have advances in computer science and microfabrication spurred the rapid development of precision mass-quantifying approaches. Here we discuss available techniques for quantifying the mass of single live cells with an emphasis on relative features, capabilities and drawbacks for different applications.

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Section: Figurementioning

confidence: 99%

Live-cell mass profiling: an emerging approach in quantitative biophysics

2014

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“…Simply, Eq (1) means that the OPD signal is proportional to both the cell thickness and the intracellular refractive index, a property linked to the protein and water content of the cells [33][34][35]. DHM systems generally use a low intensity laser as a light source for specimen illumination and a digital camera to record the hologram.…”

Section: Label-free Digital Holographic Imagingmentioning

confidence: 99%

Automated multi-parameter measurement of cardiomyocytes dynamics with digital holographic microscopy

Rappaz¹,

Moon²,

Yi³

et al. 2015

Opt. Express

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Abstract:Compounds tested during drug development may have adverse effects on the heart; therefore all new chemical entities have to undergo extensive preclinical assessment for cardiac liability. Conventional intensity-based imaging techniques are not robust enough to provide detailed information for cell structure and the captured images result in lowcontrast, especially to cell with semi-transparent or transparent feature, which would affect the cell analysis. In this paper we show, for the first time, that digital holographic microscopy (DHM) integrated with information processing algorithms automatically provide dynamic quantitative phase profiles of beating cardiomyocytes. We experimentally demonstrate that relevant parameters of cardiomyocytes can be obtained by our automated algorithm based on DHM phase signal analysis and used to characterize the physiological state of resting cardiomyocytes. Our study opens the possibility of automated quantitative analysis of cardiomyocyte dynamics suitable for further drug safety testing and compounds selection as a new paradigm in drug toxicity screens.

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“…Currently, only few methods have been demonstrated for measuring the dry mass of a single cell based on transmission electron microscopy 3 ͑TEM͒ or optical interferometry. [4][5][6] The TEM method, is too complicated and is not suitable for a living cell. Cell interferometry is the most general method for measuring the cell dry mass, which is calculated from the integral of the optical path difference of a living cell.…”

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confidence: 99%

“…As there exists a linear relationship between the dry masses density and refractive index of the cell, the cell dry and water masses can be calculated from the value of volume and refractive index of the cell. 6 The optical system of the measurement setup is illustrated in Fig. 1.…”

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confidence: 99%

Determination of single living cell’s dry/water mass using optofluidic chip

Song

Liu

Swaminathan

et al. 2007

Applied Physics Letters

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This paper presents an interferometric method for measuring the dry/wet mass of a single living cell using an optofluidic chip. It consists of the fiber Mach-Zehnder interferometer and the fiber-optical trap, both of which are integrated onto a single chip. In experiment, a single living cell is captured by the fiber-optical trap, and then the cell’s refractive index and diameter are simultaneously determined by the spectral shift in response to the buffer modulation. Hence, both cell dry and water masses can be calculated with a precision higher than 5%.

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Determination of Dry Mass, Thickness, Solid and Water Concentration in Living Cells

Cited by 172 publications

References 1 publication

Live-cell mass profiling: an emerging approach in quantitative biophysics

Live-cell mass profiling: an emerging approach in quantitative biophysics

Automated multi-parameter measurement of cardiomyocytes dynamics with digital holographic microscopy

Determination of single living cell’s dry/water mass using optofluidic chip

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