Determination of estradiol valerate in pharmaceutical preparations and human serum by flow injection chemiluminescence

Abstract

A novel method for the detection of trace estradiol valerate (EV) in pharmaceutical preparations and human serum was developed by inhibition of luminol chemiluminescence (CL) by estradiol valerate on the zinc deuteroporphyrin (ZnDP)-enhanced luminol-K3 Fe(CN)6 chemiluminescence system. Under optimized experimental conditions, CL intensity and concentration of estradiol valerate had a good linear relationship in the ranges of 8.0 × 10(-8) to 1.0 × 10(-5) g/mL. Detection limit (3σ) was estimated to be 3.5 × 10(-8) g/mL. The proposed method was applied successfully for the determination of estradiol valerate in pharmaceutical preparations and human serum and recoveries were 97.0-105.0% and 95.5-106.0%, respectively. The possible mechanism of the CL system is discussed.

Flow injection chemiluminescence immunosensor for the determination of clenbuterol by immobilizing coating-antigen on carboxylic resin beads

“…It possesses many advantages, such as being simple, less time consuming and easy to automate for high sample throughput. Therefore, it has been widely applied in various elds, including environmental monitoring, 14 food safety, 15 pharmaceutical analysis, 16,17 identication of bacteria 18 and clinical diagnosis. 19,20 The combination of FIA detection with chemiluminescence [21][22][23] will be one of the most useful detection techniques for improving cumbersome, time-consuming, and labor-intensive traditional immunoassays.…”

Temperature-Driven Isosymmetric Reversible Phase Transition of the Hormone Estradiol 17β Valerate

“…There are many pharmaceutical products containing E2 in association with progestagen, such as E2V plus dienogest (DNG), used to enhance the effectiveness in preventing pregnancy, once natural E2 causes poor cycle control. In this way, concerning E2V, the literature reports several methods to determine this active pharmaceutical ingredient (API), most of them describing it in association with other hormones, for quality control of this drug. − …”

Flow‐injection chemiluminescence method to detect a β2 adrenergic agonist

“…Recently, several reports have described the application of FI–CL analysis to different formulations such as powder‐injection , tablet , injection and capsule . Combined with pretreatment technology, several FI–CL methods had been developed to detect drugs in biological samples . In addition, Fe 2+ , azoxystrobin and nitrite have been rapidly detected using the FI–CL method.…”