Determination of ethambutol by a sensitive fluorescent probe

Wang, Wenying; Yang, Jiyuan; Du, Liming; Wu, Hao; Li, Changfeng

doi:10.1016/j.saa.2011.02.045

Cited by 25 publications

(12 citation statements)

References 36 publications

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“…The fluorescence of berberine can serve as fluorescent marker. In addition, both absorption and emission of berberine are sensitive to environment and can be used for sensing [34,[37][38][39][40][41][42]. Fluorescence spectra of berberine, ND, ND-berberine complex are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

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Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Lin¹,

Lin²,

Tsai³

et al. 2017

JBPE

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Abstract. The natural isoquinoline alkaloid berberine has been demonstrated for its significant activity against a variety of deceases caused by bacterial and parasite infections. Nanodiamond (ND) has also been proven to have great potential for drug delivery. In this work, the complex of ND with berberine is constructed through surface functionalized ND, aiming for drug delivery applications. Spectroscopic analysis was performed on the complex interaction with human lung alveolar carcinoma epithelial cell (A549) and red blood cell (RBC) in comparison with berberine in solution. The studied cells were treated with carboxylated ND, ND-berberine complex and berberine, respectively. Their distributions in cells structures are visualized using ND and berberine's fluorescence signals for detection. The distribution of ND and ND-berberine complex differs with the distribution of berberine introduced in solution form. The results show that the ND with attached drug molecules can act as a drug carrier allowing controllable localization of the drug into the cell. This result can have implication in drug delivery, release and delivery tracing applications. © 2017 Journal of Biomedical Photonics & Engineering.Keywords: Nanodiamond; Berberine; Bio-Applications; Medical Applications; Fluorescence; Surface Functionality; Imaging; Drug delivery; Nanobiotechnology. targeted nanoparticles as specific Helicobacter pylori eradication therapy: in vitro and in vivo study," Nanomedicine (Lond) 10(1), 57-71 (2015). 48. P. R. Vuddanda, A. Mishra, S. K. Singh, and S. Singh, "Development of polymeric nanoparticles with highly entrapped herbal hydrophilic drug using nanoprecipitation technique: an approach of quality by design," Pharm. Dev. Technol.

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Section: Resultsmentioning

confidence: 99%

“…41]. The mechanisms are discussed, particularly, it was supposed that the interaction of berberine with these systems reduces the interaction with water (polar solvent) and hinders relaxation mechanism of the excited berberine [37].…”

Section: Resultsmentioning

confidence: 99%

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Lin¹,

Lin²,

Tsai³

et al. 2017

JBPE

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“…Moreover, the main methods currently used to detect berberine in plasma concentration and tissues disposition are high performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrum (LC-MS) 36 37 38 39 . To better understand berberine’s antiproliferation activity in hepatoma cells, we investigated the cellular uptake, distribution and localization of berberine utilizing its fluorescent molecular properties 40 41 , by both of flow cytometry and CLSM methods. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Antiproliferation of berberine is mediated by epigenetic modification of constitutive androstane receptor (CAR) metabolic pathway in hepatoma cells

Zhang

Miao

Wang

et al. 2016

Sci Rep

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Constitutive androstane receptor (CAR) regulates hepatic xenobiotic and energy metabolism, as well as promotes cell growth and hepatocarcinogenesis. Berberine is an ancient multipotent alkaloid drug which derived from Coptis chinensis plants. Here we report that berberine is able to be cellular uptake and accessible to chromatin in human hepatoma HepG2 cells. Berberine induces more apoptosis, cell cycle arrest, but less ROS production in CAR overexpressed mCAR-HepG2 cells. Moreover, berberine inhibits expressions of CAR and its target genes CYP2B6 and CYP3A4. Furthermore, berberine enhances DNA methylation level in whole genome but reduces that in promoter regions CpG sites of CYP2B6 and CYP3A4 genes under the presence of CAR condition. These results indicated that the antiproliferation of berberine might be mediated by the unique epigenetic modifying mechanism of CAR metabolic pathway, suggesting that berberine is a promising candidate in anticancer adjuvant chemotherapy, due to its distinct pharmacological properties in clinic.

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“…Consequently, the need for a selective, precise, accurate, analytical method is urgent. The literature reveals quantitative spectrofluorometric and voltammetric methods 4,5 for the assay of RIF and INH, separately and combined in bulk, pharmaceutical formulations and human serum. Different analytical procedures for the simultaneous determination of the 4-FDC containing RIF, INH, PYP and EMB involving spectrophotometry [6][7][8][9][10] , and liquid chromatography [11][12][13][14][15][16][17] were reported.…”

Section: Introductionmentioning

confidence: 99%

A Novel Quantitative Method for the Simultaneous Assay of Rifampicin (RIF), Isoniazid (INH), Ethambutol (EMB), and Pyrazinamide (PYP) in 4-FDC Tablets

Hagga¹,

Sultana²

2016

Orient. J. Chem

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This paper presents the development and validation of a new ultra high performance chromatography (UHPLC) procedure for the simultaneous determination of isoniazid (INH), rifampicin (RIF), pyrazinamide (PYP), and ethambutol hydrochloride (EMB.2HCl) in four fixed-dose combination (4-FDC) anti-tubercular tablets. It is a dual wavelength single method in which, INH, RIF, and PYP were detected at 230 nm, whereas EMB was detected at 210 nm. The chromatographic separation was performed on Water's Acquity BEHC18 (50 mm x 2.1 mm, 1.7 µm particle size) column at a flow rate of 0.4 ml/min, 40 o C temperature, and a mobile phase consisting of triethylamine in phosphate buffer of pH 6.8, 95% and acetonitrile 5%. The proposed method was validated in terms of linearity, interday and intraday precision, recovery, specificity, and robustness. The results of the assay of the active constituents in two different batches of tablets were 102.3±1.5, 104.9±1.2, 101.0±1.4, 100.2±1.6, and 104.8±1.3, 103.8±1.1, 101.4±1.7, and 99.9±2.1, for INH, RIF, PYP, and EMB, respectively. The proposed method proves that the application of UHPLC technique is rewarding and additional economic benefits are gained as a result of reduction of analysis time and the consumption of the expensive HPLC grade materials.

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Determination of ethambutol by a sensitive fluorescent probe

Cited by 25 publications

References 36 publications

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Antiproliferation of berberine is mediated by epigenetic modification of constitutive androstane receptor (CAR) metabolic pathway in hepatoma cells

A Novel Quantitative Method for the Simultaneous Assay of Rifampicin (RIF), Isoniazid (INH), Ethambutol (EMB), and Pyrazinamide (PYP) in 4-FDC Tablets

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