Determination of Ethanol In Alcoholic Beverages by Liquid Chromatography Using the UV Detector

Betz, Joseph M.; Nikelly, J. G.

doi:10.1093/chromsci/25.9.391

Cited by 19 publications

(9 citation statements)

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“…Alkohol merupakan kelompok senyawa organik dengan ciri khas adanya gugus hidroksi pada atom karbon. Alkohol merupakan senyawa yang suka deteksi dan untuk proses pendeteksian alkohol memerlukan instrumen yang cukup mahal seperti penggunaan Gas Chromatography-Mass Spectroscopy (Sharma, 2017), High Performance liquid chromatography -UV Vis (HPLC -UV Vis) (Nikelly & Betz, 1987) Selain menggunakan instrumentasi yang mahal penggunaan instrument memerlukan waktu yang cukup Panjang, biaya perawatan instrumen yang tinggi sehingga dinilai tidak ekonomis dalam deteksi alkohol (Reis et al, 2009).…”

Section: Pendahuluanunclassified

Silver nanoparticle based alcohol sensor manufacturing training for detection of halal drinks

Yoedistira

Afthoni

Alfanaar

2021

ABDIMAS J. Pengabdi. Masy. Univ. Merdeka Malang

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Nowadays, halal drinks are one of the sectors that get the attention of various parties. Alcohol in beverages is important in determining the halalness of a beverage. Halal detection can be done using chemical sensors. In the Pharmacy Department, knowledge of analysis using sensors is rare. Therefore, this community service program was carried out to introduce qualitative methods of quick and simple alcohol analysis. The participants are students of the Pharmacy Department of STIKES Anwar Medika in Sidoarjo City. Participants are communities engaged in the health sector who have an interest in halal and how to make sensors for halal testing. This program is implemented by making a video on how to make a chemical sensor for alcohol detection. The chemical sensor is a paper-immobilized colloidal silver-based chemical sensor that is easy to apply in various conditions. Based on the results of the color change produced on the filter paper, from green to bluish which indicates that the food or beverage sample contains alcohol. Based on the questionnaire, participants expressed satisfaction with the increase in knowledge about additional tools that are easy and fast to detect alcohol.

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Section: Pendahuluanunclassified

Silver nanoparticle based alcohol sensor manufacturing training for detection of halal drinks

Yoedistira

Afthoni

Alfanaar

2021

ABDIMAS J. Pengabdi. Masy. Univ. Merdeka Malang

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“…A range of analytical methods has been reported in the literature for the determination of ethanol in foodstuffs, some of which have employed gas chromatography (GC) with FID or MS detectors (Dorubet et al, 2009 Due to its properties, ethanol cannot be directly determined by high-performance liquid chromatography using UV or uorescence detection, therefore, only a few HPLC methods have been reported that use a refractive index (RI) detector (Avila et al, 2018;Martin et al, 1986), a combination of UV-ame ionization detectors (FID) (Yarita et al, 2002). Direct analysis of ethanol was reported by adding a low concentration of a UV-absorbing compound, such as acetone, that coelutes with the ethanol peak which is then detected as a negative peak (Betz and Nikelly, 1987). However, these methods have only been applied to matrices of relatively low complexity such as alcoholic beverages and gasoline.…”

Section: Introductionmentioning

confidence: 99%

A novel, rapid and sensitive HPLC method for the determination of ethanol in non- alcoholic beverages with pre-column derivatization and simultaneous UV and fluorescence detection

Albaseer

Dören

2022

Preprint

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Determination of the ethanol content in non-alcoholic beverages is driven by health concerns and is often imposed by regulating agencies. In this paper, we present the development of a simple and sensitive HPLC method for the determination of ethanol in Juices and beverages. Ethanol undergoes precolumn derivatization using 9-Fluorenylmethyl chloroformate (Fmoc-Cl). The resulting derivative has the property that it can be detected using both UV and fluorescence detectors, which makes ethanol quantification in complex matrices more accessible. LOD and LOQ were 0.004 g/L and 0.01 g/L %, for fluorescence detector, and 0.15 g/L and 0.5 g/L, for UV detector, respectively. The mean recovery ranged from 98–109% with a relative standard deviation (RSD%) of 3.7%. This approach to ethanol determination is novel and the method is so sensitive that only a 100 µL sample volume is required and the reaction product (derivative) can be directly injected without extraction or pre-concentration. The resulting derivative has the property that it can be detected using both UV and fluorescence. Ethanol is usually determined by GC, thus, the present method serves as an alternative to current GC methods.

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“…Numerous chemical and enzymatic methods have been described to determine ethanol in biological materials [2][3][4][5]. Methods for sample preparation are static and dynamic headspace [6][7][8], and solidphase microextraction [3,9], while chemical analytical methods are gas chromatography (GC) [6,7,10,11], infrared spectroscopy (IR) [12] and high-performance liquid chromatography (HPLC) [13,14].…”

Section: Introductionmentioning

confidence: 99%

Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application

Kostić

Vujović

Milosavljevic

2021

Hem Ind

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A simple, cost-effective and fast headspace gas chromatography method coupled with flame ionization detection (HS-GC/FID) for determination of ethanol was developed and validated for clinical and forensic toxicology purposes. HS-GC/FID is often used for alcohol determination in different biological and non-biological samples. The calibration dependence of the method was linear in the range from 0.15 to 4.00 g dm-3 (r2=0.999) with adequate accuracy (99-106 %) and precision. The limit of detection (LOD) was 0.006 g dm-3. The method was quantitative (LOQ) above 0.020 g dm-3. The new method was successfully used for determination of ethanol in biological samples of intoxicated patients, car accidents participants, participants in criminal acts, and postmortem samples, non-biological samples such as alcoholic beverages, alcohol-based herbal preparations, cosmetic preparations, etc. This method is easy to perform, making it suitable not only for the routine applications in clinical biochemistry and forensic laboratories, but also in different fields of industry (e.g. for pharmaceutical preparations, cosmetics, dietary supplements, etc.). Some of the applications for ethanol determination in different samples related to various clinical-forensic cases are presented.

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Determination of Ethanol In Alcoholic Beverages by Liquid Chromatography Using the UV Detector

Cited by 19 publications

References 0 publications

Silver nanoparticle based alcohol sensor manufacturing training for detection of halal drinks

Silver nanoparticle based alcohol sensor manufacturing training for detection of halal drinks

A novel, rapid and sensitive HPLC method for the determination of ethanol in non- alcoholic beverages with pre-column derivatization and simultaneous UV and fluorescence detection

Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application

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