Determination of ethinylestradiol and gestodene in oral contraceptives by micellar electrokinetic chromatography

Abstract

SummaryA micellar electrokinetic chromatographic method is presented which permits quantification of ethinylestradiol and gestodene in pharmaceutical products. Separation was carried out at 25~ and 25 KV, using a 20 mM borate buffer (pH 9.2), 15 mM sodium dodecylsulfate in 30% acetonitrile-water (v/v). Under these conditions analyses were carried out in 7 min. Four different oral contraceptives were analysed and the results compared favourably with those of a reference liquid chromatographic method.

Voltammetric determination of ethinylestradiol at a carbon paste electrode in the presence of cetyl pyridine bromine

“…Spectrophotometry [3][4][5][6], liquid chromatography [7,8], gas chromatography [9][10][11][12][13], high-performance liquid chromatography [14][15][16][17], micellar electrokinetic capillary chromatography [18,19], isotope dilution mass spectrometry [20], electrophoresis [21], immunoassay [22,23] and chromatographic separation with amperometry detection [24] have been employed for the determination of ethinylestradiol. Although sensitive and selective, they are time-consuming, expensive and require skilled personnel and therefore are unsuitable for on-line or field monitoring.…”

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

“…Based on these, it is predictable that due to the minimum requirements of sample pretreatment, low costs and high speed MEKC can be a real alternative to HPLC in steroid analysis as demonstrated in a comparative study using betamethasone dipropionate, clotrimazole and their related substances as the model compounds. 202 Some of the several new applications of MEKC to pharmaceutical steroid analysis are listed here: the determination of ethinylestradiol and levonorgestrel 203 or gestodene 204 in oral contraceptives, hydrocortisone and its acetate together with various antibiotics, 205 prednisolone, Zn- 208 betamethasone in dissolution tests. 209 In the above-listed studies (and the majority of those not mentioned here) sodium dodecyl sulfate (SDS) was used as micelle forming agent in the background electrolyte.…”

Square-wave voltammetric determination of drospirenone and ethinylestradiol in pharmaceutical dosage form using square wave technique

“…Another RP-HPLC method was developed for the concurrent analysis of 17-beta-estradiol and Drosp in combined dosage form [7]. Moreover, numerous analytical methods were developed for the determination of EE alone in dosage form or in human plasma including spectrophotometric methods [8][9][10][11][12][13][14][15], HPTLC methods [16][17][18], liquid chromatographic methods [14,[19][20][21][22][23][24][25][26][27][28][29][30][31][32][33], micellar electrokinetic capillary chromatography (MEKC) [34,35], spectrofluorometric methods [36,37] and voltammetric techniques [38,39]. Moreover, few analytical techniques were developed for the simultaneous estimation of the studied drugs in tablet formulations including an HPTLC method [40] and chromatographic methods including an official one in United States Pharmacopoeia (USP) [41][42][43][44][45][46].…”