This study investigated the antioxidant activity of radish seed oil (RSO) and its
effects on the quality and storage characteristics of pork patties. To assess
the antioxidant capacity of RSO, this study analyzed fatty acid composition,
peroxide value (PV), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging
activity. Pork patties were manufactured with the addition of RSO—0.4%,
0.8%, 1.6%, and 2.4%—and measured in terms of proximate composition, pH,
water holding capacity (WHC), cooking loss (CL), color, texture profile
analysis, and a sensory evaluation. Total microbial count (TMC), volatile basic
nitrogen (VBN), thiobarbituric acid reactive substances (TBARS), and PV were
measured at 1, 3, and 7 days of refrigerated storage. The DPPH radical
scavenging activity of RSO was found to be 75.46%. In the cases of WHC and CL,
there was no significant differences observed between RSO0.4%, RSO0.8%, and
positive control (PC; p>0.05). Meanwhile, RSO2.4% showed significantly
lower hardness, springiness, gumminess, and chewiness than PC (p<0.05),
and these values tended to decrease with the addition of increasing RSO. In
terms of storage characteristics, with an increase in the amount of RSO added,
TMC, VBN, TBARS, and PV all decreased; among the treatment groups, RSO2.4%
showed the lowest values. In conclusion, RSO exhibits antioxidant activity, but
when added in large amounts, it negatively affects the quality characteristics
of patties while positively impacting their storage properties, thus
necessitating a balanced consideration of both outcomes. Therefore, adding 1.6%
RSO is considered to be the most appropriate level for formulations to be used
in practice.