Determination of fibrinogen in plasma by high-performance immunoaffinity chromatography

McConnell, Joseph P.; Anderson, David J.

doi:10.1016/0378-4347(93)80291-b

Cited by 16 publications

(7 citation statements)

References 32 publications

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“…Examples of these analytes include organochlorinated compounds [127], warfarin [128], b 2 -microglobulin [129], argininosuccinase [130], fibrinogen [131], interleukins [132,133], HSA [121,134], IgG [135], immunoglobulin E (IgE) [136], lymphocyte receptors [137], anti-idiotypic antibodies [138,139], interferon [133,140], and tumor necrosis factor a [133], along with polychlorinated biphenyls (PCBs) and related compounds [127]. Most of these examples have used absorbance measurements to detect eluting solutes.…”

Section: Use Of Silica and Affinity Ligands For Direct Detectionmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

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The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.

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Section: Use Of Silica and Affinity Ligands For Direct Detectionmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

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show abstract

“…Immunoaffinity supports that are used for HPIAC must be rigid and have higher efficiency than typical low-performance supports [30]. Examples of materials that have been used in HPIAC include derivatized silica [31], glass [32], azalactone beads [33], methacrylate polymeric supports [34] and polystyrene-based perfusion media [35]. …”

Section: Basic Components Of Iacmentioning

confidence: 99%

Immunoaffinity Chromatography: An Introduction to Applications and Recent Developments

2010

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Immunoaffinity chromatography (IAC) combines the use of LC with the specific binding of antibodies or related agents. The resulting method can be used in assays for a particular target or for purification and concentration of analytes prior to further examination by another technique. This review discusses the history and principles of IAC and the various formats that can be used with this method. An overview is given of the general properties of antibodies and of antibody-production methods. The supports and immobilization methods used with antibodies in IAC and the selection of application and elution conditions for IAC are also discussed. Several applications of IAC are considered, including its use in purification, immunodepletion, direct sample analysis, chromatographic immunoassays and combined analysis methods. Recent developments include the use of IAC with CE or MS, ultrafast immunoextraction methods and the use of immunoaffinity columns in microanalytical systems.

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“…The dotted line indicates the times during which the application buffer (pH 7.0) and elution buffer (pH 2.1) were passed through the column. Adapted with permission from McConnell and Anderson (49 ).…”

Section: Direct Analyte Detection By Affinity Chromatographymentioning

confidence: 99%

“…One such example (i.e., the determination of fibrinogen in human plasma) is illustrated in Fig. 1 (49 ). In this particular case, the amount of fibrinogen in the retained peak was determined by the measurement of its absorbance at 280 nm.…”

Section: Immunoaffinity Chromatographymentioning

confidence: 99%

Affinity Chromatography: A Review of Clinical Applications

Hage

1999

Clinical Chemistry

229

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Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the use of boronate, lectin, protein A or protein G, and immunoaffinity supports for the direct quantification of solutes. Newer techniques that use antibody-based columns for on- or off-line sample extraction are examined in detail, as are methods that use affinity chromatography in combination with other analytical methods, such as reversed-phase liquid chromatography, gas chromatography, and capillary electrophoresis. Indirect analyte detection methods are also described in which immunoaffinity chromatography is used to perform flow-based immunoassays. Other applications that are reviewed include affinity-based chiral separations and the use of affinity chromatography for the study of drug or hormone interactions with binding proteins. Some areas of possible future developments are then considered, such as tandem affinity methods and the use of synthetic dyes, immobilized metal ions, molecular imprints, or aptamers as affinity ligands for clinical analytes.

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Determination of fibrinogen in plasma by high-performance immunoaffinity chromatography

Cited by 16 publications

References 32 publications

Applications of silica supports in affinity chromatography

Applications of silica supports in affinity chromatography

Immunoaffinity Chromatography: An Introduction to Applications and Recent Developments

Affinity Chromatography: A Review of Clinical Applications

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