Determination of fifteen nucleotides in cultured human mononuclear blood and umbilical vein endothelial cells by solvent generated ion-pair chromatography

Simultaneous analysis of eight nucleoside triphosphates in cell lines by liquid chromatography coupled with tandem mass spectrometry

“…After SPE, 50 L of stable isotope labelled solution C1, C3 or C5 (Table 1), 10 L of Br-ATP solution and 40 L of eluent A (15) were added to corresponding SPE eluates before their evaporation.…”

“…However, the most common elution mechanisms in ion-exchange liquid chromatography require the use of non-volatile high salt concentrations in the mobile phase incompatible with mass spectrometry (MS) detection although this detection mode should be relevant in term of sensitivity and specificity. The UV detection mode, compatible with this type of chromatography, leads to a lack of sensitivity and specificity for intracellular measurements of nucleotides [8,11,[13][14][15][16][17].…”

Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas

“…Excellent resolution can be achieved because the ionic nature of the phosphate esters facilitates strong interactions with cationic ion-pair reagents at the appropriate pH (Brown, Robb, & Gelhart, 2002;Werner, 1993). Thus, IP-RPC has been employed to determine the nucleotide content in various mammalian milks and dairy products (Ferreira, Mendes, Gomes, Faria, & Ferreira, 2001;Mateo, Peters, & Stein, 2004;Oliveira, Ferreira, Mendes, & Ferreira, 1999;Perrin, Meyer, Mujahid, & Blake, 2001;Sugawara, Sato, Nakano, Idota, & Nakajima, 1995), in food ingredients (Fish, 1991) and in blood (Cichna et al, 2003).…”

Liquid chromatographic methods for the determination of endogenous nucleotides and nucleotide analogs used in cancer therapy: A review

“…The analysis of nucleotides have been performed in various matrix such as whole blood [38,39], erythrocytes [40], peripheral blood mononucleous cells (PBMC) [41][42][43], cultured cells [4,42,[44][45][46][47][48][49], cerebrospinal fluid (CSF) [50] or tissue [51][52][53]. These matrixes contain enzymes involved in the purines and pyrimidines metabolism.…”

“…For protein precipitation, perchloric acid (PCA) was mainly employed when analysis were performed either with LC-UV or LC-MS devices [38,39,42,45,47,52,53,55,56] (Tables 1 and 2). This step was followed by adding a basic solution such as KOH [38,44,45,47], K 2 HPO 4 [42], K 2 CO 3 [38] or borate buffer [39] in order to precipitate perchlorates and to neutralize the supernatant.…”