Determination of five di-(2-ethylhexyl)phthalate metabolites in urine by UPLC–MS/MS, markers of blood transfusion misuse in sports

Monfort, Núria; Ventura, Rosa; Balcells, Georgina; Segura, Jordi

doi:10.1016/j.jchromb.2012.09.030

Cited by 32 publications

(20 citation statements)

References 42 publications

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“…The metabolite concentrations in the control phase were generally low: only one volunteer exhibited high DEHP metabolite concentrations and then only at one time point (Day 0). The means and medians of the five metabolites in the control phase were similar to the concentrations in a Spanish population cohort that were reported by Monfort and colleagues . This suggests that contamination of DEHP from foods and cosmetics is rare.…”

Section: Discussionsupporting

confidence: 85%

“…The urinary DEHP metabolites MEHP, 5OH‐MEHP, 5oxo‐MEHP, 5cx‐MEPP, and 2cx‐MMHP were quantified by using the LC‐MS/MS method as described previously . The LC‐MS/MS system was a XEVO‐TQ‐S (Waters, Milford, MA).…”

Section: Methodsmentioning

confidence: 99%

“…This method centers on the plasticizer molecule di‐(2‐ethylhexyl) phthalate (DEHP) that is used to make PVC blood bags soft and pliable and that emanates from the bag during storage. DEHP and its metabolites in urine can be detected by liquid chromatography coupled to mass spectrometry (LC‐MS) with acceptable sensitivity …”

mentioning

confidence: 99%

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Urinary di‐(2‐ethylhexyl) phthalate metabolites for detecting transfusion of autologous blood stored in plasticizer‐free bags

et al. 2015

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Section: Discussionsupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

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Urinary di‐(2‐ethylhexyl) phthalate metabolites for detecting transfusion of autologous blood stored in plasticizer‐free bags

et al. 2015

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“…would be at higher risk due to their more frequent exposure to high levels of DEHP. It has even been suggested to use DEHP and its metabolites in the human body as markers for blood transfusion misuse in sports (Monfort et al 2012(Monfort et al , 2010. The article by Koch et al (2005) provides an overview of the breakdown pathway of DEHP in the human body.…”

Section: Routes Of Human Exposure To Dehpmentioning

confidence: 99%

Leaching of the plasticizer di(2-ethylhexyl)phthalate (DEHP) from plastic containers and the question of human exposure

Erythropel

Marić

Nicell

et al. 2014

Appl Microbiol Biotechnol

360

182

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Di(2-ethylhexyl)phthalate (DEHP) is a widely used plasticizer to render poly(vinyl chloride) (PVC) soft and malleable. Plasticized PVC is used in hospital equipment, food wrapping, and numerous other commercial and industrial products. Unfortunately, plasticizers can migrate within the material and leach out of it over time, ending up in the environment and, frequently, the human body. DEHP has come under increased scrutiny as its breakdown products are believed to be endocrine disruptors and more toxic than DEHP itself. DEHP and its breakdown products have been identified as ubiquitous environmental contaminants, and daily human exposure is estimated to be in the microgram per kilogram level. The objective of this review is to summarize and comment on published sources of DEHP exposure and to give an overview of its environmental fate. Exposure through bottled water was examined specifically, as this concern is raised frequently, yet only little exposure to DEHP occurs through bottled water, and DEHP exposure is unlikely to stem from the packaging material itself. Packaged food was also examined and showed higher levels of DEHP contamination compared to bottled water. Exposure to DEHP also occurs in hospital environments, where DEHP leaches directly into liquids that passed through PVC/DEHP tubing and equipment. The latter exposure is at considerably higher levels compared to food and bottled water, specifically putting patients with chronic illnesses at risk. Overall, levels of DEHP in food and bottled water were below current tolerable daily intake (TDI) values. However, our understanding of the risks of DEHP exposure is still evolving. Given the prevalence of DEHP in our atmosphere and environment, and the uncertainty revolving around it, the precautionary principle would suggest its phaseout and replacement. Increased efforts to develop viable replacement compounds, which necessarily includes rigorous leaching, toxicity, and impact assessment studies, are needed before alternative plasticizers can be adopted as viable replacements.

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“…[172] Using enzymatic hydrolysis followed by LLE, target analytes were separated on a C-18 analytical column (2.1 x 100 mm, particle size 1.7 μm) and determined in positive and negative ESI with MRM detection. In a follow-up study, Monfort et al reported on an LC-MS/MS methodology allowing the quantification of the main five metabolites of one of the most common plasticizers di-(2-ethylhexyl)phthalate (DEHP).…”

Section: Manipulation Of Blood and Blood Componentsmentioning

confidence: 99%

Annual banned‐substance review: analytical approaches in human sports drug testing

Thevis

Kuuranne

Geyer

et al. 2013

Drug Testing and Analysis

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Monitoring the misuse of drugs and the abuse of substances and methods potentially or evidently improving athletic performance by analytical chemistry strategies is one of the main pillars of modern anti-doping efforts. Owing to the continuously growing knowledge in medicine, pharmacology, and (bio)chemistry, new chemical entities are frequently established and developed, various of which present a temptation for sportsmen and women due to assumed/attributed beneficial effects of such substances and preparations on, for example, endurance, strength, and regeneration. By means of new technologies, expanded existing test protocols, new insights into metabolism, distribution, and elimination of compounds prohibited by the World Anti-Doping Agency (WADA), analytical assays have been further improved in agreement with the content of the 2013 Prohibited List. In this annual banned-substance review, literature concerning human sports drug testing that was published between October 2012 and September 2013 is summarized and reviewed with particular emphasis on analytical approaches and their contribution to enhanced doping controls.

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Determination of five di-(2-ethylhexyl)phthalate metabolites in urine by UPLC–MS/MS, markers of blood transfusion misuse in sports

Cited by 32 publications

References 42 publications

Urinary di‐(2‐ethylhexyl) phthalate metabolites for detecting transfusion of autologous blood stored in plasticizer‐free bags

Urinary di‐(2‐ethylhexyl) phthalate metabolites for detecting transfusion of autologous blood stored in plasticizer‐free bags

Leaching of the plasticizer di(2-ethylhexyl)phthalate (DEHP) from plastic containers and the question of human exposure

Annual banned‐substance review: analytical approaches in human sports drug testing

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