Determination of five sesquiterpenoids in Xingnaojing injection by quantitative analysis of multiple components with a single marker

Abstract

A quantitative analysis of multiple components with a single-marker method was established for the simultaneous determination of five sesqutiterpenoids in Xingnaojing injection. This method was established with Xingnaojing injection determined by high-performance liquid chromatography coupled with diode array detection. The durability and system suitability of the established method were evaluated, and the reliable relative correction factors were obtained with curdione selected as an internal reference. The contents of the five components in all Xingnaojing injections were determined by external standard method and the contents of curcumenone, curcumenol, curzerenone, and germacrone were also calculated with the obtained relative correction factors. Then, relative error was investigated to estimate the difference of the two methods. As a result, the established new method possesses good adaptability, and there is no significant difference between the two methods, except for the content of curzerenone in eight samples. To put the established method into practice, the limits of quantitation of the established method of the five components were proposed and defined. Thus, the developed methodology can also be utilized to the quality evaluation of Xingnaojing injection, in spite of the difference found in the content of curzerenone between the external standard method and the newly established method.

Investigating chemical features of Panax notoginseng based on integrating HPLC fingerprinting and determination of multiconstituents by single reference standard

“…In these studies, multiple reference standards are needed for quantitative determination of these constituents with routine methods, i.e., external standard method. However, shortage, cost and instability of chemical reference standards are issues that should be considered [22] , [23] . With this limitation in mind, determination of multiconstituents by single reference standard (DMS) is an outstanding solution.…”

Probing the degradation mechanism of forsythiaside A and simultaneous determination of three forsythiasides in Forsythia preparations by a single marker

“…Only one reference substance is used in this method for the determination of multi‐components and for simultaneously quantifying the contents of other analytes by using the relative correction factors. At present, QAMS has been widely recognized and applied in the determination of multi‐components in traditional Chinese medicine and preparations .…”

Single standard substance for the determination of nine volatile components in the distillate of Fructus Gardeniae and Radix Curcumae (an intermediate of Xingnaojing Injection)

“…The reference substances of some of the volatile components in FG and RC are very expensive; therefore, the commonly used external standard method (ESM) with multiple reference substances would be cost prohibitive. Quantitative analysis of multi‐component with a single‐marker (QAMS) , also called single standard to determine multi‐components , is a method proposed in recent years to overcome the scarcity and high cost of reference substances. In QAMS, only one reference substance is used for the determination of multiple analytes, taking advantage of the relative correction factors (RCFs) between the analytes.…”

“…In QAMS, only one reference substance is used for the determination of multiple analytes, taking advantage of the relative correction factors (RCFs) between the analytes. The applications of QAMS for the QC of traditional Chinese medicines have been reported in the literature [8][9][10][11][12][13][14] and have also been accepted by the current Chinese Pharmacopoeia [15].…”