Determination of flavonoids and ascorbic acid in grapefruit peel and juice by capillary electrophoresis with electrochemical detection

Wu, Ting; Guan, Yueqing; Ye, Jiannong

doi:10.1016/j.foodchem.2005.12.042

Cited by 224 publications

(106 citation statements)

References 18 publications

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“…It is grown on an area of 23057 feddans and produced about146469 tones (FAO, 2014). Orange is considered as a main source of antioxidants like vitamin C, phenolic compounds and carotenoids, so it has an important functions in resistance against many human illnesses (Del Caro et al, 2004); (DhuiqueMayer et al, 2005) and (Wu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of some Pre- and Postharvest Treatments on the Ability of “Balady” Orange Fruits to Storage

2017

AJAS

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Abstract:The experiment was conducted during two successive seasons (2015 and 2016) on Balady orange trees (Citrus sinensis) which divided in two groups. The first group was sprayed with potassium sulphate (48%) in the orchard at three times sequential (March, June and August) and the second group was sprayed with water (control). Orange fruits of both treatments were collected in maturity stage and dipped in salicylic acid (SA) at 4mM/l in laboratory. The purpose of this investigation is studying the effect of SA and K on orange fruits quality during storage at room temperature (13+2 C°). Some physical and chemical properties of fruits were estimated every 10 days till 50 days (the end of storage) such as weight loss %, juice content%, acidity%, total soluble solids% (TSS), TSS/ acid ratio and ascorbic acid during both seasons. The results illustrated that, SA had a positive effect on fruit quality of the fruits treated with SA single or combined with potassium sulphate.

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Section: Introductionmentioning

confidence: 99%

Effect of some Pre- and Postharvest Treatments on the Ability of “Balady” Orange Fruits to Storage

2017

AJAS

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“…3,4 In this context, various analytical methods have been reported in literature for determination of some flavonoids. These include UV-Vis absorption, spectrophotometry, 5 chromatographic methods like thinlayer chromatography (TL), gas chromatography (GC), liquid chromatography (LC) and capillary electrophoresis (CE) with UV or electrochemical detection, [6][7][8][9][10][11] based on the oxidation of the phenolic hydroxyl groups in flavonoids. Direct electroanalytical determination of flavonoids by differential pulse voltammetry or stripping techniques has only rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

Square-wave cathodic adsorptive stripping voltammetric determination of 3-hydroxyflavone, Morin and Hesperidin in bulk form and biological fluids in absence and presence of Cu(II)

Temerk¹,

Ibrahim²,

Kotb³

2011

J. Braz. Chem. Soc.

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Devido à atividade farmacológica dos flavonóides, na presente investigação, foi desenvolvido um método eficiente e econômico para a determinação de traços dos mesmos. Neste contexto, o comportamento interfacial de três flavonóides, 3-hidroxiflavona (3HF), morin e hesperidina (Hesp) foi estudado em eletrodo de gota pendente de mercúrio (HMDE) por voltametria ac (fase sensível) e voltametria cíclica (CV). Os flavonóides investigados mostraram-se fortemente absorvidos, o que é um pré-requisito para a aplicação de voltametria adsortiva de redissolução catódica, na determinação de alguns flavonóides. Baseado no caráter de adsorção dos flavonóides investigados, na superfície de HMDE, foi descrito um procedimento de voltametria adsortiva de redissolução catódica com onda quadrada (SWCASV), validado, simples, rápido e sensível, para a quantificação dos flavonóides sob investigação na forma granel e em fluidos biológicos. Além disso, a acumulação adsortiva controlada do complexo de Cu(II) de flavonóides foi também obtida e usada para a determinação indireta de 3HF, morin e Hesp via redução do complexo formado. As condições operacionais e de solução para a determinação quantitativa ultra-traço dos compostos flavonóides investigados, foram otimizadas na ausência e presença de Cu(II). Os limites de detecção de 4,4 × 10 -9 , 7,19 × 10 -9 e 7,54 × 10 -9 mol L -1 para 3HF, morin e Hesp, respectivamente, na forma granel, foram atingidos usando SWCASV.As flavonoids show pharmacological activity, in the present investigation, an economical and efficient method for their trace determination was developed. In this context, the interfacial behavior of three flavonoids, 3-hydroxyflavone (3HF), morin and hesperidin (Hesp) was studied on the hanging mercury dropping electrode (HMDE) by phase sensitive ac voltammetry and cyclic voltammetry (CV). The investigated flavonoids were strongly adsorbed which is the prerequisite step for applying the cathodic adsorption stripping voltammetric determination of some flavonoids. Based on the adsorption character of investigated flavonoids onto the surface of the HMDE, validated, simple, fast and sensitive square-wave cathodic adsorptive stripping voltammetric (SWCASV) procedure for the quantification of the flavonoids under investigation in bulk form and in biological fluids was described. Moreover, controlled adsorptive accumulation of the Cu(II) complex of flavonoids was also achieved to assay indirect determination of 3HF, morin and Hesp via the reduction of the formed complex. Operational and solution conditions for the quantitative ultra-trace determination of the investigated flavonoids were optimized in the absence and presence of Cu(II). Limits of detection of 4.4 × 10 -9 , 7.19 × 10 -9 and 7.54 × 10 -9 mol L -1 3HF, morin and Hesp in bulk form were achieved using SWCASV, respectively.

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“…[26][27][28] Hyphenated instruments consisting of flow injection analysis, [29][30][31][32] high performance liquid chromatography [33][34][35] or capillary electrophoresis [36][37][38][39] instruments and a detector are mostly utilized for the determination of Vitamic C. Liquid chromatography is a successful method for vitamin C determination when selectivity and specificity are concerned. [40][41][42] The objective of the present study was to determine the relative bioavailability of synthetic Vitamin C and Nutra-C ® in Korean healthy volunteers.…”

Section: Introductionmentioning

confidence: 99%

Comparative bioavailability of synthetic vitamin C and Nutra-C (calcium ascorbate) in Korean healthy volunteers

Choi¹,

Hoon²,

Won³

et al. 2016

Analytical Science and Technology

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The purpose of this study was to compare the relative bioavailability of synthetic Vitamin C and Nutra-C ® (calcium ascorbate) using a randomized parallel pharmacokinetics study design. Under fasting conditions, 20 healthy volunteers were randomly allocated to receive a single oral dose (500 mg of ascorbic acid) of either synthetic Vitamin C or Nutra-C ® . Fasting blood was collected pre-dose and 1, 2, 3, 4, 7 and 10 hr post-dose. The ascorbic acid content of human serum was determined using HPLC with ultraviolet detection. The fasting serum ascorbic acid concentrations of synthetic Vitamin C and Nutra-C ® were 6.734 ± 2.09 ng/mL (n = 10) and 7.542 ± 2.96 ng/mL (n = 10), respectively. The bioavailability of Nutra-C ® was significantly greater (128 %, p < 0.05) than that of the synthetic Vitamin C.

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Determination of flavonoids and ascorbic acid in grapefruit peel and juice by capillary electrophoresis with electrochemical detection

Cited by 224 publications

References 18 publications

Effect of some Pre- and Postharvest Treatments on the Ability of “Balady” Orange Fruits to Storage

Effect of some Pre- and Postharvest Treatments on the Ability of “Balady” Orange Fruits to Storage

Square-wave cathodic adsorptive stripping voltammetric determination of 3-hydroxyflavone, Morin and Hesperidin in bulk form and biological fluids in absence and presence of Cu(II)

Comparative bioavailability of synthetic vitamin C and Nutra-C (calcium ascorbate) in Korean healthy volunteers

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