Determination of fluoxetine enantiomers in pharmaceutical formulations by electrokinetic chromatography–counter current technique

Asensi-Bernardi, Lucía; Martı́n-Biosca, Yolanda; Fornet-Herrero, Eder; Sagrado, S.; Medina-Hernández, M.J.

doi:10.1002/bmc.2802

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…In order to prevent the absorption of the basic compound fluoxetine on the negatively charged capillary wall in low pH buffer and to improve enantioresolution, guanidine as cationic additive can be added to a phosphate buffer containing sulfobuthylether-β-CD at pH 2.5 (Javid et al, 2013). An electrokinetic chromatography–counter current procedure for the separation of fluoxetine enantiomers using a phosphate buffer at pH 8.0 and highly sulfated β-CD was developed and applied to the determination of the enantiomers in pharmaceutical formulations (Asensi-Bernardi et al, 2013). Sulfated maltodextrin as a novel anionic chiral selector was used as an alternative to CDs for the separation of several basic drugs including fluoxetine (Tabani et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis

Cârcu-Dobrin

Budău

Hancu

et al. 2017

Saudi Pharmaceutical Journal

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Fluoxetine is an antidepressant, a selective serotonin reuptake inhibitor (SSRI) used primarily in the treatment of major depression, panic disorder and obsessive compulsive disorder. Chiral separation of racemic fluoxetine is necessary due to its enantioselective metabolism. In order to develop a suitable method for chiral separation of fluoxetine, cyclodextrin (CD) modified capillary electrophoresis (CE) was employed. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector. As a result of this process, heptakis(2,3,6-tri-O-methyl)-β-CD (TRIMEB) was selected for enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors are varied at the same time to optimize the separation method. The optimized method (50 mM phosphate buffer, pH = 5.0, 10 mM TRIMEB, 15 °C, + 20 kV, 50 mbar/1 s, detection at 230 nm) was successful for baseline separation of fluoxetine enantiomers within 5 min. Our method was validated according to ICH guidelines and proved to be sensitive, linear, accurate and precise for the chiral separation of fluoxetine.

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Section: Introductionmentioning

confidence: 99%

Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis

Cârcu-Dobrin

Budău

Hancu

et al. 2017

Saudi Pharmaceutical Journal

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“…Among these methods, each exhibits disadvantages that limit its application, such as complicated derivatization steps for fluorescence detection, poor sensitivity for UV detection, and expensive equipment for MS detection. CE has become a widely used technique in drug analysis because of its higher separation efficiency, unique separation selectivity, rapidity, smaller sample volume, and lower costs because of low solvent consumption. The major drawback of CE is its low sensitivity, particularly when it is coupled with a UV detector.…”

Section: Introductionmentioning

confidence: 99%

Dispersive liquid–liquid microextraction combined with acetonitrile stacking through capillary electrophoresis for the determination of three selective serotonin reuptake inhibitor drugs in body fluids

Lin

Chiu

Hsieh

2016

J of Separation Science

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Dispersive liquid-liquid microextraction was combined with acetonitrile stacking in capillary electrophoresis for the identification of three selective serotonin reuptake inhibitors (citalopram, fluoxetine, and fluvoxamine) in human fluids such as urine and plasma. Parameters that affect the extraction and stacking efficiency, such as the type and volume of the extraction and disperser solvent, extraction time, salt addition for dispersive liquid-liquid microextraction, and sample matrices, pH, and concentration of the separation buffer for stacking, were investigated and optimized. Under optimum conditions, the enrichment factors were in the range of 1195-1441. Limits of detection ranged from 1.4 to 1.7 nM for the target analytes. Calibration graphs displayed satisfied linearity with R greater than or equal to 0.9978, and relative standard deviations of the peak area analysis were in the range of 2.9-5.0% (n = 3). The recoveries of all tricyclic antidepressant drugs from urine and plasma were in the range of 77-117 and 79-106%, respectively. The findings of this study show that dispersive liquid-liquid microextraction acetonitrile-stacking capillary electrophoresis is a rapid and convenient method for identifying tricyclic antidepressant drugs in urine and plasma.

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“…Asensi‐Bernardi, Martin‐Biosca, Fornet‐Herrero, Sagrado, and Medina Hernández () developed an enantioselective method for the analysis of FLX enantiomers in pharmaceuticals by electrokinetic chromatography–counter‐current technique using highly sulfated‐ β ‐CD (HS‐ β ‐CD) as chiral selector. In this mode, the entire capillary was filled with the chiral selector, while the inlet and outlet vials were also free of chiral selector in partial‐filling technique.…”

Section: Methodsmentioning

confidence: 99%

Analytical methodologies for the stereoselective determination of fluoxetine: An overview

Hancu

Cârcu-Dobrin

Budău³

et al. 2017

Biomedical Chromatography

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Fluoxetine is a widely used antidepressant belonging to the selective serotonin reuptake inhibitor class; it is used in the treatment of major depression, obsessive compulsive, premenstrual dysphoric, panic and post-traumatic stress disorders. Fluoxetine is an optical active pharmaceutical substance, which is used as a racemate in therapy, but stereospecific interactions associated with the serotonin-reuptake carrier, for both the parent drug and its active metabolite, norfluoxetine, have been described in the literature. Therefore, the stereoselective analysis of fluoxetine and norfluoxetine is important in order to characterize the pharmacokinetic and pharmacodynamic profile of the analytes. Several chromatographic and electrophoretic methods have been published in the literature for the chiral discrimination of fluoxetine enantiomers from different matrices. The purpose of the current review is to provide a systematic survey of the analytical techniques used for the chiral determination of fluoxetine and norfluoxetine covering a period of~25 years.

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Determination of fluoxetine enantiomers in pharmaceutical formulations by electrokinetic chromatography–counter current technique

Cited by 12 publications

References 21 publications

Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis

Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis

Dispersive liquid–liquid microextraction combined with acetonitrile stacking through capillary electrophoresis for the determination of three selective serotonin reuptake inhibitor drugs in body fluids

Analytical methodologies for the stereoselective determination of fluoxetine: An overview

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