Determination of free amino acids in microalgae by high‐performance liquid chromatography using pre‐column fluorescence derivatization

Abstract

An analytical method for a rapid reverse-phase liquid chromatography of amino acids is presented. The total analysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% and the fluorescent response was linear with concentration for OPA derivatives (r > 0.99) in the range 100-1000 nM. The proposed method was applied to the determination of free amino acids in two microalgae species (Chlorella pyrenoidosa and Chlamydomonas rinhardtii). The principal aminoacids found were alanine, arginine leucine, serine and glutamic acid.

A novel amino acid analysis method using derivatization of multiple functional groups followed by liquid chromatography/tandem mass spectrometry

“…6 For example, the presence of N,N-dimethylglycine, N-formyl-L-methionine, L-pyroglutamic acid, and sarcosine, which are amino group-modified amino acids, has been reported to be related to various diseases. [7][8][9][10][11][12] Many methods for amino acid analysis using liquid chromatography (LC) have been reported, including precolumn derivatization-reversed phase chromatography, 13,14 anion exchange chromatography, 15 ion-pair chromatography, 16 and hydrophilic interaction liquid chromatography. 4,5,17,18 The chromatographic separation of many kinds of amino acids in the human body can take a long time, which severely limits high-throughput analysis.…”

MEKC: A powerful tool for the determination of amino acids in a variety of biomatrices

“…For example, the measurement of trace levels of these components in biological media is often used to study control and regulation processes in living organisms or to predict the likely course of a disease and to monitor and assist in the management of the disease 10–17. Among the variety of methods that have been developed for their detection 18–34, CE has easily emerged as an excellent technique. The publication over the last two decades of a large number of interesting articles/reviews focused on the application of CE to the separation/detection of amino acid mixtures, has provided considerable evidence that both CZE and MEKC have a great power of attraction in this area 35–40.…”