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Papaya bunchy top (PBT) disease has been associated with Rickettsia-related proteobacterium. To study the occurrence of this proteobacterium in Cuba, during 2012, we collected 264 samples from asymptomatic and symptomatic papaya trees in three regions of Cuba. Symptomatic tissue evaluated by transmission electron microscopy revealed Rickettsia-like morphology. PCR using rickettsial sdhA-specific primers amplified a single band with expected size from 95 % of symptomatic plants. Phylogeny confirmed the presence of Rickettsia-related proteobacterium in plant showing PBT disease. This work confirms Rickettsia-related proteobacterium is a generalized pathogen in papaya plant affected by bunchy top disease in Cuba.

An analytical method for a rapid reverse‐phase liquid chromatography of amino acids is presented. The total analysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% and the fluorescent response was linear with concentration for OPA derivatives (r>0.99) in the range 100–1000 nM. The proposed method was applied to the determination of free amino acids in two microalgae species (Chlorella pyrenoidosa and Chlamydomonas rinhardtii). The principal aminoacids found were alanine, arginine leucine, serine and glutamic acid. © 1997 John Wiley & Sons, Ltd.

An analytical method for a rapid reverse-phase liquid chromatography of amino acids is presented. The total analysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% and the fluorescent response was linear with concentration for OPA derivatives (r > 0.99) in the range 100-1000 nM. The proposed method was applied to the determination of free amino acids in two microalgae species (Chlorella pyrenoidosa and Chlamydomonas rinhardtii). The principal aminoacids found were alanine, arginine leucine, serine and glutamic acid.