Determination of free amino acids by high performance liquid chromatography

Schuster, R.

doi:10.1021/ac50054a005

Cited by 91 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All separations were performed with a 5–50 % gradient of solvent A using a flow rate of 1 ml/min. The amino acids were detected at a wavelength of 200 nm (Schuster 1980). All the peptides originated from different groups that consistently contained the following three amino acids: phenylalanine (Phe), cysteine (Cys), and lysine (Lys).…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Blackcurrant on Liver Cell Membrane of Rats Intoxicated with Ethanol

et al. 2012

View full text Add to dashboard Cite

Chronic ethanol intoxication oxidative stress participates in the development of many diseases. Nutrition and the interaction of food nutrients with ethanol metabolism may modulate alcohol toxicity. One such compound is blackcurrant, which also has antioxidant abilities. We investigated the effect of blackcurrant as an antioxidant on the composition and electrical charge of liver cell membranes in ethanol-intoxicated rats. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were determined by high-performance liquid chromatography. Electrophoresis was used to determine the surface charge density of the rat liver cell membranes. Ethanol intoxication is characterized by changes in cell metabolism that alter the structure and function of cell membrane components. Ethanol increased phospholipid levels and altered the level of integral proteins as determined by decreased phenylalanine, cysteine, and lysine. Ethanol significantly enhanced changes in the surface charge density of the liver cell membranes. Administration of blackcurrant to rats intoxicated with ethanol significantly protected lipids and proteins against oxidative modifications. It is possible that the beneficial effect of blackcurrant is connected with its abilities to scavenge free radicals and to chelate metal ions.

show abstract

Section: Methodsmentioning

confidence: 99%

Protective Effect of Blackcurrant on Liver Cell Membrane of Rats Intoxicated with Ethanol

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Samples of 2.5 to 3 mL of blood were drawn into dry vacuum tubes, frozen, and kept at ‐20°C until analyzed. Amino acids were determined by the phenyl‐isothiocyanate (PITC) method 10,11 . PITC derivatives were detected at 254 nm.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Subcutaneous and Intravenous Administration of a Solution of Aminoacids in Older Patients

Ferry¹,

Leverve²,

Constans³

1997

J American Geriatrics Society

View full text Add to dashboard Cite

show abstract

“…Para identificar la citrulinemia se utiliz6 cromatografia unidimensional de aminodcidos en papel, con tinci6n de Ehrlich 7 , confirm^ndola por medici6n cuanlitativa con analizador dc aminoicidos 9 . Las concentraciones de amonio en la sangre se determinaron con un mStodo reflectom6trico 8 1. Var6n, segundo hijo de padres j6venes, sanos, no consanguincos.…”

Section: Pacientes Y Metodosunclassified

Citrulinemia

B¹,

E²,

D³

et al. 1994

Rev. chil. pediatr.

View full text Add to dashboard Cite

CitrulinemiaClinical, laboratory and follow up feolures of three patients whith Citrulinemia lhat was identified at ages three months, seven days and seven months are described. AH Ihese palients had abnormal blood amonia (> 200 JJg/dl] and their serum citruline concentrations were 353, 1 759, 289 nn/ml respectively. Nutritional treatment induced a low protein, high calories diet with L-carnitine (100 mg x kg x day), L-arginine [70 to 1 20 mg x kg x day), together with mineral and vitamine supplements. Earlier clinical symptoms are related to more severe argininosuccinale synthetase deficiency and to worse biochemical abnormalities and clinical course.Only one of our palients had ihe acute neonatal form of ihe disease and he was also the one that had the highest blood and urine citruline levels.(

show abstract

Determination of free amino acids by high performance liquid chromatography

Cited by 91 publications

References 13 publications

Protective Effect of Blackcurrant on Liver Cell Membrane of Rats Intoxicated with Ethanol

Protective Effect of Blackcurrant on Liver Cell Membrane of Rats Intoxicated with Ethanol

Comparison of Subcutaneous and Intravenous Administration of a Solution of Aminoacids in Older Patients

Citrulinemia

Contact Info

Product

Resources

About