2009
DOI: 10.1002/yea.1707
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Determination of fruit origin by using 26S rDNA fingerprinting of yeast communities by PCR–DGGE: preliminary application to Physalis fruits from Egypt

Abstract: The determination of geographical origin is a demand of the traceability system of import-export food products. One hypothesis for tracing the source of a product is by global analysis of the microbial communities of the food and statistical linkage of this analysis to the geographical origin of the food. For this purpose, a molecular technique employing 26S rDNA profiles generated by PCR-DGGE was used to detect the variation in yeast community structures of three species of Physalis fruit (Physalis ixocarpa B… Show more

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Cited by 64 publications
(55 citation statements)
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“…The extractions of DNA were made according to the protocols of El Sheikha et al [14,15]. All the experiments were done in duplicate.…”
Section: Extraction Of Microbial Dna From Bissap Samplesmentioning
confidence: 99%
See 3 more Smart Citations
“…The extractions of DNA were made according to the protocols of El Sheikha et al [14,15]. All the experiments were done in duplicate.…”
Section: Extraction Of Microbial Dna From Bissap Samplesmentioning
confidence: 99%
“…The final volume was 50 µL and it consisted of 5 µL of 10X Buffer (Promega), 3 µL of MgCl 2 , 1 µL deoxyribonucleotide triphosphates (dNTPs) and 10 µL of primer solution (0.2 µM). The primers were GC-338F (5´-GCG CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG GCA TCC TAC GGG AGG CAG CAG-3´, Sigma) and 518R (5´-ATT ACC GCG GCT GCT GG-3´, Sigma) for bacteria [16]; GC-U1 (5´-CGC CCG CCG CGC GCG GCG GGC GGG GGG GTG AAA TTG TTG AAA GGG AA-3´, Sigma) and U2 (5´-GAC TCC TTG GTC CGT GTT-3´, Sigma) for molds [15] and GC-NL1 (5´-CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCC ATA TCA ATA AGC GGA GGA AAA G-3´, Sigma) and LS2 (5´-ATT CCC AAA CAA CTC GAC TC-3´, Sigma) for yeasts [14]. Taq polymerase (Promega) 0.25 µL, 1−5 µL DNA and 15.75−19.75 µL pure water (5 PRIME Gmbh, Hamburg, Germany) were added.…”
Section: Analysis Of the Microbial Flora By Pcr-dgge (Denaturing Gradmentioning
confidence: 99%
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“…In case of doubt or fraud, it is necessary to find a precise and rapid analytical technique to determine their geographical origin. 31 The PCR-DGGE method of analysis is a unique way to generically identify all microbial flora (bacteria, yeast, moulds) present on fruit, in order to create the linkage of microbial communities to the geographical origin and avoid the individual analysis of each strain. The acquired band patterns for the microbial communities of different species of fruits and different harvesting locations were compared and analysed statistically to determine the fruit geographical origin.…”
Section: Pcr-dgge Technique In the Traceability Of Fruitsmentioning
confidence: 99%