Determination of Fumonisins B1 and B2 in Herbal Tea and Medicinal Plants in Turkey by High-Performance Liquid Chromatography

Abstract

The purpose of this study was to measure the potential levels of fumonisin B1 (FB1) and fumonisin B2 (FB2) contamination in several herbal teas and medicinal plants that are consumed regularly in Turkey. FB1 and FB2 were detected using high-performance liquid chromatography with fluorescence detection after derivatization with o-phthaldialdehyde. A total of 115 commercially available herbal tea and medicinal plant samples were analyzed. The recoveries in black tea were 86.9+/-8.42% for FB1 and 102+/-6.80% for FB2 spiked with 1 microg/g of each analyte. Similarly, the mean recovery results in lime (linden) for FB1 and FB2 were 85.2+/-9.76% and 78.6+/-5.67%, respectively. The minimum detectable amounts for the o-phthaldialdehyde derivatives of FB1 and FB2 were 0.025 microg/g (1 ng injected) and 0.125 microg/g (5 ng), respectively. FB1 was detected in two samples (0.160 and 1.487 microg/g), and FB2 was detected in none of the samples.

Mycotoxins in botanicals and dried fruits: A review

“…Fumonisins in medicinal plants were determined by methanol-water extraction, strong ion exchange solid-phase extraction column clean-up, o-phthaldialdehyde derivatization, reversed-phase LC separation, and FL detection (Martins et al 2001;Omurtag and Yazicioglu 2004). The LODs for the derivatization methods were 20-25 mg kg À1 .…”

“…In Turkey, a total of 115 herbal tea and medicinal plant samples were analysed using an LC method. FB 1 was detected in two samples at 160 and 1487 mg kg À1 and no FB 2 was found (Omurtag and Yazicioglu 2004). In South Africa, 30 medicinal plants were analysed for FB 1 and aflatoxin B 1 (AFB 1 ) using immunoaffinity column (IAC) clean-up and LC with fluorescence (FL) detection (Sewram et al 2006).…”

LC-MS/MS multi-analyte method for mycotoxin determination in food supplements

“…Indeed, several surveys of toxigenic moulds in botanicals have found high levels of Aspergillus, Penicillium and Fusarium species (Abeywickrama and Bean 1991;Halt 1998;Rizzo et al 2004). While the presence of mould might not be correlated with the presence of mycotoxins, there are reports of aflatoxins (Rizzo et al 1999;Tassaneeyakul et al 2004;Yang et al 2005;Ali et al 2005;D'Ovidio et al 2006;Trucksess et al 2007), OTA (Thirumala-Devi et al 2001;Trucksess et al 2007), ZEN (Gray et al 2004) and fumonisins (Martins et al 2001;Omurtag and Yazicioğlu 2004;Sewram et al 2006) in medicinal plants, tea and other botanicals. Contamination of these raw materials Currently analytical methods used for mycotoxin analysis include thin layer chromatography (TLC) (Betina 1993;, enzyme-linked immunosorbent assay (ELISA) (Ware et al 1999;Thirumala-Devi et al 2001;Heber et al 2001), gas chromatography (GC) with electron capture (Langseth and Rundberget 1998) or mass spectrometric (Schwadorf and Müller 1992;Langseth and Rundberget 1998;Valenta 1998;Shephard 1998;Tanaka et al 2000;Nielsen and Thrane 2001;Soleas et al 2001) detection, liquid chromatography with fluorescence detection (LC-FLD) (Valenta 1998;Shephard 1998;, and liquid chromatography with tandem mass spectrometry (LC-MS/MS) (Young and Lafontaine 1993;Thakur and Smith 1994;Biselli et al 2005).…”

Occurrence of Fumonisins B1 and B2 in homemade medicinal plants: Exposure assessment in northern Turkey

“…), raspberry (Rubus sanctus), and coriander (Coriandrum sativum) were dried under shade and studied to evaluate the presence of FB 1 and FB 2 . Analyses were carried out with regard to the reported HPLC method after OMURTAG and YAZICIOĞLU (2004) as given below.…”

“…), Nirenberg (ex F. moniliforme Sheldon) and Fusarium proliferatum. They are associated with maize and are frequently present in naturally contaminated corn, feeds, medicinal and other plants throughout the world; particularly where a high humidity exists (CASTELO et al, 1998;MARTINS et al, 2001a;SOLFRIZZO et al, 2001;OMURTAG, 2001;OMURTAG & YAZICIOĞLU, 2004).…”