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This study was conducted to determine the recent level of contamination with Fumonisin B 1 (FB 1 ) and Fumonisin B 2 (FB 2 ) in major medicinal plants and to assess consumer exposure in northern Turkey. FB 1 and FB 2 were investigated by using high performance liquid chromatography (HPLC) with fl uorescence detection after derivatization with o-phthaldialdehyde (OPA). A total of 78 homemade medicinal plant samples from 14 species were analysed. The recovery in thyme was 67.2±5.2% for FB 1 and 80.8±14.3% for FB 2 spiked with 1 μg g -1 of each analyte. The minimum detectable amount for the OPA derivatives of FB 1 and FB 2 were 1 ng per injection and 2.5 ng per injection, respectively. The limit of quantitation (LOQ) S/N=10 was 0.078 and 0.313 μg g -1 , and the limit of detection (LOD) S/N=3 was 0.023 and 0.093 μg g -1 for FB 1 and FB 2 , respectively. FB 1 was detected in thyme (0.125) and mint (0.125 and 0.256 μg g -1 ) samples; however. FB 2 toxin was below the detection limit in all samples. These results indicate that toxins might be present in homemade medicinal plants; however, the risk of exposure to fumonisins by the consumption of those plants was lower than the estimated TDI limits (<2 μg kg -1 bw).