Determination of Furazolidone and Nitrofurazone in Chicken Tissues

Abstract

F A method for the determination of furazolidone and nitrofurazone in chicken tissues is based on the formation and colorimetric estimation of 5nitro-2-furaldehyde phenylhydrazone. A chromatographic separation of the phenylhydrazone from nonspecific liver chromogens permits visual demonstration of the nitrofuran in concentrations as low as 0.5 p.p.m. and permits quantitative determination of these nitrofurans in chicken liver at 1 to 5 p.p.m. with an accuracy of 92 to 104%. A nonchromatographic method is used for fat and muscle residue analysis. The recoveries for these tissues ranged from 97 to 1 0 8~o at 1 and 5 p.p.m. HE INCREASING use of furazolidone, T 3 -(5nitrofurfurylideneamino) -2oxazolidinone (Furoxone), and nitrofurazone, 5-nitro-Zfuraldehyde semicarbazone (Furacin), (Structures 1 and 2) in the treatment and control of various poultry diseases (5, 7) has em-Structure 1. Nitrofurazone, 5-nitro-2-furaldehyde semicarbazone

Furazolidone concentrations in plasma, milk and some tissues of Nubian goats

“…T h e colorimetric methods of Buzard et al (1956) and Herrett 8c Buzard (1960) for estimating FZ are not specific as they d o not differentiate between the parent drug and its metabolites. However, the HPLC method of Ernst & Van der Kaaden (1980) used in this investigation is both specific and sensitive.…”

“…Using the colorimetric method of Herrett & Buzard (1960), with a limit of detection of 0.5-5 pg g-', no FZ concentrations could be detected in any of the tissues examined. However, using the HPLC method of Ernst & Van der Kaaden (1980) with a limit of detection of 0.05 pg g-', FZ was detected in the gluteal muscle and liver at concentrations of 0.26 f 0.01 and 0.10 k 0.02 pg g-I, respectively, 24 h after the end of the dosage period (10 mg/kg, 5 days).…”

“…Goats were killed 1 , 3 , 5 , 7 and 10 days after the end of the dosage period, and samples (25 g) of the gluteal muscle, liver, kidney and heart were quickly removed. T h e concentrations of FZ in the samples were determined by the colorimetric method of Herrett & Buzard (1960). and by the high-performance liquid chromatography (HPLC) method of Ernst & Van der Kaaden (1980).…”

Metabolites of Furazolidone in Urine of Chickens

“…portion of the toluene phase was mixed with 1 g. of aluminum oxide in a centrifuge tube. The 5-nitro-2-furfuraldehydephenylhydrazone adsorbed t o the alumina, showing a red color which was a qualitative test for that specific hydrazone (9). T o elute the hydrazone.…”

Absorption and Distribution of Oxafuradene in the Dog

“…Apparently, urine pigments produced colored materials which interferred with the procedure. Bile samples were analyzed by a modificationof themethod of Herrett and Buzard (9).…”