Determination of furosemide and its glucuronide metabolite in plasma, plasma ultrafiltrate and urine by HPLC-MS/MS with application to secretion and metabolite formation clearances in non-pregnant and pregnant women

Benzi, Jhohann Richard de Lima; Rocha, Adriana; Colombari, Julia Cristina; Pego, Alef Machado Gomes; dos Santos Melli, Patrícia Pereira; Duarte, Geraldo; Lanchote, Vera Lucia

doi:10.1016/j.jpba.2023.115635

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…It works by inhibiting the Na + , K + , and 2Cl cotransporter in the thick ascending limb of the loop of Henle, resulting in increased water excretion together with sodium, chloride, and potassium (5)(6)(7). A variety of techniques have been described for the determination of (FUR) in Pharmaceutical Dosage by samples (HPLC) (8,9), atomic absorption and UV-visible spectrophotometry (10), voltammetry (11,12), and LC-ESI-MS (13) . Particles having a structural size of 100 nm or less are referred to as nanoparticles (14), Silver Nanoparticles used in its unique and antibacterial properties, is widely used in several applications Nanosilver is utilized in medical devices, clothes, cosmetics, electronics, biosensing, and other products (15).…”

Section: Introductionmentioning

confidence: 99%

Green nano-coated carbon electrodes for detecting furosemide drug

Mohammed,

Khaleel,

Al-Samarrai

2024

Sensors and Machine Learning Applications

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In this study, new coated carbon electrodes were created and utilized to estimate the furosemide (FUR) drug. Ion-pair preparation for the electrode construction involved a reaction of furosemide drug with silver nanoparticles (Ag-NPs) made from pomegranate peel extract, plasticizers that use dibutyl phthalate (DBP) without the use of any organic precipitant. Several techniques (AFM, XRD, FTIR, SEM) were used to characterize the characteristics. This electrode shows excellent sensitivity to furosemide (FUR) with a linear range of 10-6 -10-1 M and a correlation coefficient of 0.9798. The electrode has a lifetime of 45 days, ideal temperatures of 20-45 ºC, an optimum pH range of 3-6, a slope of 61.286 mv/decade, and a detection limit of 3.62×10-8 M. The AFM was measured, the total height was 2.53 nm, and the shape was spherical or semi-spherical. The average crystal size measured by XRD was 43.52, 47, and 55.03 nm with an average size of 14.25067 nm; the particles were spherical or semi-spherical in shape, as seen using the SEM. The identification of functional aggregates was conducted by measuring them using FTIR. In conclusion, this method could be an efficient tool for determining the drug in pharmaceutical formulations.

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Section: Introductionmentioning

confidence: 99%

Green nano-coated carbon electrodes for detecting furosemide drug

Mohammed,

Khaleel,

Al-Samarrai

2024

Sensors and Machine Learning Applications

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The Impact of Inflammation on the In Vivo Activity of the Renal Transporters OAT1/3 in Pregnant Women Diagnosed with Acute Pyelonephritis

Benzi,

Melli,

Duarte

et al. 2023

Pharmaceutics

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Inflammation can regulate hepatic drug metabolism enzymes and transporters. The impact of inflammation on renal drug transporters remains to be elucidated. We aimed to quantify the effect of inflammation (caused by acute pyelonephritis) on the in vivo activity of renal OAT1/3, using the probe drug furosemide. Pregnant women (second or third trimester) received a single oral dose of furosemide 40 mg during acute pyelonephritis (Phase 1; n = 7) and after its resolution (Phase 2; n = 7; by treatment with intravenous cefuroxime 750 mg TID for 3–7 days), separated by 10 to 14 days. The IL-6, IFN-γ, TNF-α, MCP-1, and C-reactive protein plasma concentrations were higher in Phase I vs. Phase II. The pregnant women had a lower geometric mean [CV%] furosemide CLsecretion (3.9 [43.4] vs. 6.7 [43.8] L/h) and formation clearance to the glucuronide (1.1 [85.9] vs. 2.3 [64.1] L/h) in Phase 1 vs. Phase 2. Inflammation reduced the in vivo activity of renal OAT1/3 (mediating furosemide CLsecretion) and UGT1A9/1A1 (mediating the formation of furosemide glucuronide) by approximately 40% and 54%, respectively, presumably by elevating the plasma cytokine concentrations. The dosing regimens of narrow therapeutic window OAT drug substrates may need to be adjusted during inflammatory conditions.

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Determination of furosemide and its glucuronide metabolite in plasma, plasma ultrafiltrate and urine by HPLC-MS/MS with application to secretion and metabolite formation clearances in non-pregnant and pregnant women

Cited by 2 publications

References 27 publications

Green nano-coated carbon electrodes for detecting furosemide drug

Green nano-coated carbon electrodes for detecting furosemide drug

The Impact of Inflammation on the In Vivo Activity of the Renal Transporters OAT1/3 in Pregnant Women Diagnosed with Acute Pyelonephritis

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