Determination of gallic acid in Phyllanthus emblica Linn. dried fruit powder by HPTLC

Abstract

Objective:Emblica (Phyllanthus emblica L.), an euphorbiaceous plant, is widely distributed in subtropical and tropical areas of India, China and Indonesia. The fruits possess antimicrobial, antioxidant, anti-inflammatory, analgesic and antipyretic properties. In the current article a new, simple, sensitive, selective, precise, and robust high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the determination of gallic acid in dried fruit powder of Phyllanthus emblica.Materials and Methods:The quantitative determination of gallic acid was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. The linear ascending development was carried out in a twin trough glass chamber saturated with a mobile phase consisting of toluene: ethyl acetate: formic acid: methanol (3:3:0.8:0.2) at room temperature (25 ± 2°C). Camag TLC scanner III was used for spectrodensitometric scanning and analysis, in the absorbance mode, at 278 nm.Results:The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.99977 in the concentration range of 40 – 240 ng spot—1, with respect to the peak area. According to the guidelines of the International Conference on Harmonization (ICH), the method was validated for precision, accuracy, and recovery.Conclusion:Statistical analysis of the data showed that the method was reproducible and selective for the estimation of gallic acid.

Phyllanthus emblica L. Branch Extract Ameliorates Testicular Damage in Valproic Acid-Induced Rats

“…Unfortunately, this study did not show those results; however, such biochemical analyses must be further investigated to elucidate the real mechanism underlying of PE protective effects. It can be clearly explained that PE extract used in this study is rich in phenolic contents and antioxidant capacities (Iamsaard et al, 2014b), which is similar to those of the fruit parts (She et al, 2013;Chalise et al;Sawant et al, 2010). Indeed, PE extract did not affect any male reproductive organs including sperm parameters as compared to the control.…”

泰国余甘子水提物中总酚含量及抗氧化活性

“…Focusing on the fruit, it has been demonstrated that the fruits of PE are rich in phenolics (She et al, 2013) and possess antioxidant activities (Chalise et al, 2010;Sawant et al, 2010). In addition, PE fruits have been proven to have many therapeutic properties (Krishnaveni and Mirunalini, 2010;Chularojmontri et al, 2013).…”

Amelioration of Hepato-Renal Toxicity by a Flavonoid-Rich Fraction of Hibiscus rosa-sinenses (Leaves and Flowers) in Male Rabbits Intoxicated by Cadmium

“…The flavonoid-rich fraction was analyzed by thin layer chromatography (TLC) for the presence of gallic acid using a mobile phase comprised of toluene:ethyl acetate:formic acid:methanol (3:3:0.8:0.2 v/v), quercetin using toluene:ethyl acetate:formic acid (5:4:1 v/v), and rutin using ethyl acetate:n-butanol:formic acid:water (5:3:1:1 v/v) at room temperature (25±2ºC) as described as [14,18] …”